Detection of sclerotinia sclerotiorum in planta by a real-time PCR assay

Journal of Phytopathology

Volumn 157, Issue 7-8, 2009, Pages 465-469

  Yin, Yanni ^a   Ding, Laisong ^a   Liu, Xin ^a   Yang, Jinghui ^b   Ma, Zhonghua ^a  

^a ZHEJIANG UNIVERSITY   (China)

^b Zhenjiang Institute of Agricultural Science   (China)

Author keywords

Oilseed rape; Real time polymerase chain reaction; Sclerotinia sclerotiorum

Indexed keywords

BIOASSAY; DETECTION METHOD; FUNGAL DISEASE; HERB; POLYMERASE CHAIN REACTION; REAL TIME; TESTING METHOD;

BRASSICA NAPUS; FUNGI; SCLEROTINIA SCLEROTIORUM;

EID: 67651176191     PISSN: 09311785     EISSN: 14390434     Source Type: Journal    
DOI: 10.1111/j.1439-0434.2009.01543.x     Document Type: Article

References (16)

1. Boland GJ, Hall R 1994) Index of plant hosts of Sclerotinia sclerotiorum. Can J Plant Pathol 16 : 93 108.
2. Bolton MD, Thomma BHJ, Nelson BD 2006) Sclerotinia sclerotiorum (Lib.) de Bary: biology and molecular traits of a cosmopolitan pathogen. Mol Plant Pathol 7 : 1 16.
3. Bom M, Boland GJ 2000) Evaluation of disease forecasting variables for sclerotinia stem rot (Sclerotinia sclerotiorum) of canola. Can J Plant Sci 80 : 889 898.
4. Carpenter MA, Frampton C, Stewart A 1999) Genetic variation in New Zealand populations of the plant pathogen Sclerotinia sclerotiorum. NZ J Crop Hortic Sci 27 : 13 21.
5. Freeman J, Ward E, Calderon C, McCartney A 2002) A polymerase chain reaction (PCR) assay for the detection of inoculum of Sclerotinia sclerotiorum. Eur J Plant Pathol 108 : 877 886.
6. Gente S, Sohier D, Coton E, Duhamel C, Gueguen M 2006) Identification of Geotrichum canditum at the species and strain level: proposal for a standardized protocol. J Ind Microbiol Biotechnol 33 : 1019 1031.
7. Hegedus DD, Rimmer SR 2005) Sclerotinia sclerotiorum: when 'to be or not to be' a pathogen? FEMS Microbiol Lett 251 : 177 184.
8. Lumsden RD 1976) Pectolytic enzymes of Sclerotinia sclerotiorum and their localization on infected bean. Can J Bot 54 : 2630 2641.
9. Ma Z, Michailides TJ 2007) Approaches for eliminating PCR inhibitors and designing PCR primers for the detection of phytopathogenic fungi. Crop Prot 26 : 145 161.
10. Ma Z, Luo Y, Michailides TJ 2003) Nested PCR assays for detection of Monilinia fructicola in stone fruit orchards and Botryosphaeria dothidea from pistachios in California. J Phytopathol 151 : 312 322.
11. McCartney HA, Lacey ME, Li Q, Heran A. Airborne ascospore concentration and the infection of oilseed rape and sunflowers by Sclerotinia sclerotiorum. In : Wratten EN, Salisbury PA (eds Proc 10th Intern Rapeseed Congress, Canberra, Australia, September 26-29th,1999, Paris, France, Groupe Consultatif International de Recherche sur le Colza, 1999.
12. Saitoh K, Togashi K, Arie T, Teraoka T 2006) A simple method for a mini-preparation of fungal DNA. J Gen Plant Pathol 72 : 348 350.
13. Sirjusingh C, Kohn LM 2001) Characterization of microsatellites in the fungal plant pathogen, Sclerotinia sclerotiorum. Mol Ecol Notes 1 : 267 269.
14. Turkington TK, Morrall RAA, Gugel RK 1991) Use of petal infestation to forecast Sclerotinia stem rot of canola: evaluation of early bloom sampling, 1985-1990. Can J Plant Pathol 13 : 50 59.
15. Wilson IG 1997) Inhibition and facilitation of nucleic acid amplification. Appl Environ Microbiol 63 : 3741 3751.
16. Yan L, Zhang C, Ding L, Ma Z 2008) Development of a real-time PCR assay for the detection of Cladosporium fulvum in tomato leaves. J Appl Microbiol 104 : 1417 1424.