Expression of recombinant human BMP6 in CHO cells by fused to the signal peptide and propeptide of another homologue protein

Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology

Volumn 23, Issue 3, 2007, Pages 413-417

  Yan, Ji Dong ^a   Yang, Shuang ^a   Lü, Shu Jun ^a   Lei, Rong Yue ^a   Zhu, Tian Hui ^a  

^a NANKAI UNIVERSITY   (China)

Author keywords

BMP6; Bone induction; Chinese hamster ovary cell; Recombination expression

Indexed keywords

CRICETULUS GRISEUS; MAMMALIA;

ALKALINE PHOSPHATASE; BMP2 PROTEIN, HUMAN; BONE MORPHOGENETIC PROTEIN 2; BONE MORPHOGENETIC PROTEIN 6; HYBRID PROTEIN; PROTEIN PRECURSOR; SIGNAL PEPTIDE;

ANIMAL; ARTICLE; CELL LINE; CELL STRAIN COS1; CERCOPITHECUS; CHO CELL; CRICETULUS; CYTOLOGY; DRUG EFFECT; ENZYMOLOGY; GENE EXPRESSION; GENETIC TRANSFECTION; GENETICS; HAMSTER; HUMAN; METABOLISM; MYOBLAST; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

ALKALINE PHOSPHATASE; ANIMALS; BLOTTING, WESTERN; BONE MORPHOGENETIC PROTEIN 2; BONE MORPHOGENETIC PROTEIN 6; CELL LINE; CERCOPITHECUS AETHIOPS; CHO CELLS; COS CELLS; CRICETINAE; CRICETULUS; GENE EXPRESSION; HUMANS; MYOBLASTS; PROTEIN PRECURSORS; PROTEIN SORTING SIGNALS; RECOMBINANT FUSION PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TRANSFECTION;

EID: 67651159955     PISSN: 10003061     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (12)

1. Urist MR. Bone: formation by autoinduction. Science, 1965, 150(698): 893-899.
2. Gitelman SE, Kobrin MS, Ye JQ, et al. Recombinant Vgr-1/BMP-6-expressing tumors induce fibrosis and enchondral bone formation in vivo. The Journal of Cell Biology, 1994, 126(6): 1595-1609.
3. Gitelman SE, Kirk M, Ye JQ, et al. Vgr-1/BMP-6 induces osteoblast differentiation of pluripotential mesenchymal cells. Cell Growth & Differentiation, 1995, 6(7): 827-836.
4. Le AX, Miclau T, Hu D, et al. Molecular aspects of healing in stabilized and non-stabilized fractures. Journal of Orthopaedic Research, 2001, 19(1): 78-84.
5. Li JZ, Li H, Sasaki T, et al. Osteogenic potential of five different recombinant human bone morphogenetic protein adenoviral vectors in the rat. Gene Therapy, 2003, 10(20): 1735-1743.
6. Kang Q, Sun MH, Cheng H, et al. Characterization of the distinct orthotopic bone-forming activity of 14 BMPs using recombinant adenovirus-mediated gene delivery. Gene Therapy, 2004, 11(17): 1312-1320.
7. Pietro De Biase, Rodolfo Capanna. Clinical applications of BMPs. Injury, 2005, 36(S3): S43-S46.
8. Termaat MF, Den Boer FC, Bakker FC, et al. Bone morphogenetic proteins development and clinical efficacy in the treatment of fractures and bone defects. The Journal of Bone and Joint Surgery (American), 2005, 87: 1367-1378.
9. Sambrook J, Russell D. Molecular Cloning: A Laboratory Manual. 3nd eds, New York: Cold Spring Harbor Laboratory Press, 2001.
10. Yang JH, Zhao L, Yang S, et al. Expression of recombinant human BMP6 in E. coli and its purification and bioassay in vitro. Chinese Journal of Biotechnology, 2003, 19(5): 556-560.
11. Wolfman. Mutants of bone morphogenetic proteins. United States Patent, 5804416, 1998.
12. Zou ZC, Sun PD. Overexpression of human transforming growth factor-β1 using a recombinant CHO cell expression system. Protein Expression and Purification, 2004, 37(2): 265-272.