A rapid and hazardous reagent free protocol for genomic DNA extraction suitable for genetic studies in plants

Molecular Biology Reports

Volumn 36, Issue 6, 2009, Pages 1633-1636

  Kotchoni, Simeon O ^a   Gachomo, Emma W ^b  

^a PURDUE UNIVERSITY   (United States)

^b RUTGERS UNIVERSITY   (United States)

Author keywords

Arabidopsis thaliana; Genomic DNA; PCR; Protocol; Restriction enzymes

Indexed keywords

2 PROPANOL; SODIUM CHLORIDE;

ARABIDOPSIS; ARTICLE; DNA EXTRACTION; GENETIC ANALYSIS; METHODOLOGY; NONHUMAN; PLANT; PLANT GENOME; ROOM TEMPERATURE;

DNA, PLANT; GENETIC TECHNIQUES; GENOME, PLANT; PLANTS; RESEARCH DESIGN;

ARABIDOPSIS THALIANA;

EID: 67651111923     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-008-9362-9     Document Type: Article

References (18)

1. JA Couch PJ Fritz 1990 Isolation of DNA from plants high in polyphenolics Plant Mol Biol Rep 8 8 12 10.1007/BF02668875
2. B Ziegenhagen P Guillemaut F Scholz 1993 A procedure for mini-preparation of genomic DNA from needles of silver fir (Abies alba) Plant Mol Biol Rep 11 117 121 10.1007/BF02670469
3. AL Chaves CE Vergara JE Mayer 1995 Dichloromethane as an economic alternative to chloroform in the extraction of DNA from plant tissues Plant Mol Biol Rep 13 18 25 10.1007/BF02668389
4. F Luro F Laigret 1995 Preparation of high molecular weight genomic DNA from nuclei of woody plants Biotechniques 19 388 392
5. P Barnwell AN Blanchard JA Bryant N Smirnoff AF Weir 1998 Isolation of DNA from the highly mucilaginous succulent plant Sedum telephium Plant Mol Biol Rep 16 133 138 10.1023/A:1007473302551 (Pubitemid 129189847)
6. G Fang S Hammar R Grumet 1992 A quick and inexpensive method for removing polysaccharides from plant genomic DNA Biotechniques 13 52 56
7. RN Pandey RP Adams LE Flournoy 1996 Inhibition of random amplified polymorphic DNAs (RAPDs) by plant polysaccharides Plant Mol Biol Rep 14 17 22 10.1007/BF02671898
8. R Sharma SJ John DM Damgaard TA McAllister 2003 Extraction of PCR-quality plant and microbial DNA from total rumen contents Biotechniques 34 92 97
9. S Chang J Puryear J Cairney 1993 A simple and efficient method for isolating RNA from pine trees Plant Mol Biol Rep 11 113 116 10.1007/BF02670468
10. C Sperisen F Gugerli U Büchler G Mátyás 2000 Comparison of two rapid DNA extraction protocols for gymnosperms for application in population genetic and phylogenetic studies Genetics 7 133 136
11. MG Murray WF Thompson 1980 Rapid isolation of high molecular weight plant DNA Nucleic Acids Res 19 4321 4325 10.1093/nar/8.19.4321
12. MA Saghai-Maroof KM Soliman RA Jorgensen RW Allard 1984 Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics Proc Natl Acad Sci USA 81 8014 8018 10.1073/pnas.81.24.8014
13. MC Schmeerman J Mwangi B Hobart J Arbukle DA Vaske JC Register III 2002 The dried corncob as a source of DNA for PCR analysis Plant Mol Biol Rep 20 59 65 10.1007/BF02801933
14. K Edwards C Johnstone C Thompson 1991 A simple and rapid method for the preparation of plant genomic DNA for PCR analysis Nucleic Acids Res 19 1349 10.1093/nar/19.6.1349
15. SL Dellaporta J Wood JB Hicks 1983 Maize DNA minipreps Maize Gent Coop News 57 26 29
16. SL Dellaporta J Wood JB Hicks 1983 A plant DNA minipreparation: version II Plant Mol Biol Rep 1 19 21 10.1007/BF02712670
17. CA Benito M Figueiras C Zaragoza FJ Gallego A del Pena 1993 Rapid identification of Triticeae genotypes from single seeds using the polymerase chain reaction Plant Mol Biol 21 181 183 10.1007/BF00039629
18. F Guidet 1994 A powerful new technique to quickly prepare hundreds of plant extracts for PCR and RAPD analyses Nucleic Acids Res 22 1772 1773 10.1093/nar/22.9.1772