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Volumn 22, Issue 6, 2009, Pages 375-382
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Site-directed mutagenesis to probe catalysis by a Thermobifida fusca β-1,3-glucanase (Lam81A)
a
b
b
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Author keywords
Azide rescue; Catalytic base; Substrate inhibition; Substrate specificity
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Indexed keywords
AROMATIC RINGS;
AZIDE RESCUE;
CARBOXYMETHYL;
CATALYTIC ACIDS;
CATALYTIC BASE;
CONSERVED RESIDUES;
ENDOGLUCANASE;
GLUCANASE;
GLYCOSYL;
MUTANT ENZYMES;
SEQUENCE ALIGNMENTS;
SINGLE DOMAINS;
SITE DIRECTED MUTAGENESIS;
SUBSTRATE BINDING;
SUBSTRATE INHIBITION;
SUBSTRATE SPECIFICITY;
THERMOBIFIDA FUSCA;
WILD-TYPE ENZYMES;
BINDING SITES;
CATALYSIS;
ENZYME ACTIVITY;
ENZYMES;
MUTAGENESIS;
SUGAR (SUCROSE);
SUGARS;
SUBSTRATES;
1,3 BETA GLUCANASE;
AZIDE;
BETA GLUCAN;
CARBOXYMETHYLCELLULOSE;
HISTIDINE;
BACTERIAL PROTEIN;
GLUCAN ENDO 1,3 BETA GLUCOSIDASE;
SODIUM AZIDE;
ARTICLE;
BACTERIAL STRAIN;
CATALYSIS;
CONTROLLED STUDY;
ENZYME ACTIVITY;
ENZYME SPECIFICITY;
HYDROLYSIS;
MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY;
MUTATIONAL ANALYSIS;
NONHUMAN;
PRIORITY JOURNAL;
PROTEIN BINDING;
PROTEIN CLEAVAGE;
SEQUENCE ANALYSIS;
SITE DIRECTED MUTAGENESIS;
THERMOBIFIDA FUSCA;
THERMOPHILIC BACTERIUM;
THIN LAYER CHROMATOGRAPHY;
ACTINOMYCETALES;
AMINO ACID SEQUENCE;
BIOCATALYSIS;
CHEMISTRY;
ENZYME ACTIVE SITE;
ENZYMOLOGY;
GENETICS;
KINETICS;
MASS SPECTROMETRY;
METABOLISM;
MOLECULAR GENETICS;
SEQUENCE ALIGNMENT;
THERMOBIFIDA;
THERMOBIFIDA FUSCA;
ACTINOMYCETALES;
AMINO ACID SEQUENCE;
BACTERIAL PROTEINS;
BETA-GLUCANS;
BIOCATALYSIS;
CARBOXYMETHYLCELLULOSE;
CATALYTIC DOMAIN;
GLUCAN ENDO-1,3-BETA-D-GLUCOSIDASE;
KINETICS;
MOLECULAR SEQUENCE DATA;
MUTAGENESIS, SITE-DIRECTED;
PROTEIN BINDING;
SEQUENCE ALIGNMENT;
SODIUM AZIDE;
SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;
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EID: 67651102916
PISSN: 17410126
EISSN: 17410134
Source Type: Journal
DOI: 10.1093/protein/gzp015 Document Type: Article |
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Times cited : (9)
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References (21)
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