Colorimetric microbial viability assay based on reduction of water-soluble tetrazolium salts for antimicrobial susceptibility testing and screening of antimicrobial substances

Analytical Biochemistry

Volumn 393, Issue 1, 2009, Pages 117-125

  Tsukatani, Tadayuki ^a   Higuchi, Tomoko ^a   Suenaga, Hikaru ^a   Akao, Tetsuyuki ^a   Ishiyama, Munetaka ^b   Ezoe, Takatoshi ^b   Matsumoto, Kiyoshi ^c  

^a FUKUOKA INDUSTRIAL TECHNOLOGY CENTER   (Japan)

^b Dojindo Laboratories   (Japan)

^c KYUSHU UNIVERSITY   (Japan)

Author keywords

Electron mediator; Microorganism; Naphthoquinone; Screening; Susceptibility testing; Tetrazolium salt

Indexed keywords

ANTIBIOTICS; COLOR; COLORIMETRY; KETONES; LACTIC ACID; MICROORGANISMS; SCREENING;

2-METHYL-1 ,4-NAPHTHOQUINONE; ANTIMICROBIAL SUBSTANCE; ANTIMICROBIAL SUSCEPTIBILITY TESTING; ELECTRON MEDIATOR; GRAM-POSITIVE BACTERIUM; MEASUREMENT CONDITIONS; MINIMUM INHIBITORY CONCENTRATION; NAPHTHOQUINONE;

BACTERIA;

2 (2 METHOXY 4 NITROPHENYL) 3 (4 NITROPHENYL) 5 (2,4 DISULFOPHENYL) 2H TETRAZOLIUM; AMPICILLIN; ANTIBIOTIC AGENT; BACTERIOCIN; CEFOTAXIME; CHLORAMPHENICOL; CIPROFLOXACIN; GENTAMICIN; MENADIONE; TETRAZOLIUM; UNCLASSIFIED DRUG;

ANTIBIOTIC SENSITIVITY; ARTICLE; COLORIMETRY; CONCENTRATION PROCESS; CONTROLLED STUDY; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; INTERMETHOD COMPARISON; MICROBIAL VIABILITY; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; REDUCTION KINETICS; SOLUBILITY; YEAST;

NEGIBACTERIA; POSIBACTERIA;

EID: 67651094182     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.06.026     Document Type: Article

References (13)

1. Clinical and Laboratory Standards Institute, Methods for Dilution Microbial Susceptibility Tests for Bacteria that Grow Aerobically: Approved Standard, seventh ed., CLSI document M7-A7, CLSI, Wayne, PA, 2006.
2. Meletiadis J., Mouton J.W., Meis J.F., Bouman B.A., Donnelly J.P., and Verweij P.E. Colorimetric assay for antifungal susceptibility testing of Aspergillus species. J. Clin. Microbiol. 39 (2001) 3402-3408
3. Tunney M.M., Ramage G., Field T.R., Moriarty T.F., and Storey D.G. Rapid colorimetric assay for antimicrobial susceptibility testing of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 48 (2004) 1879-1881
4. Brady A.J., Kearney P., and Tunney M.M. Comparative evaluation of 2,3-bis [2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) and 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfophenyl)-2H-tetrazolium, monosodium salt (WST-8) rapid colorimetric assays for antimicrobial susceptibility testing of staphylococci and ESBL-producing clinical isolates. J. Microbiol. Methods 71 (2007) 305-311
5. Moriartya F., Elbornb S., and Tunney M. Development of a rapid colorimetric time-kill assay for determining the in vitro activity of ceftazidime and tobramycin in combination against Pseudomonas aeruginosa. J. Microbiol. Methods 61 (2005) 171-179
6. Berridge M.V., Herst P.M., and Tan A.S. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol. Annu. Rev. 11 (2005) 127-152
7. Paull K.D., Shoemaker R.H., Boyd M.R., Parsons J.L., Risbood P.A., Barbera W.A., Sharma M.N., Baker D.C., Hand E., Scudiero D.A., Monks A., Alley M.C., and Grote M. The synthesis of XTT: a new tetrazolium reagent that is bioreducible to a water-soluble formazan. J. Heterocyclic Chem. 25 (1988) 911-913
8. Scudiere D.A., Shoemaker R.H., Paull K.D., Monks A., Tierney S., Nofziger T.H., Currens M.J., Seniff D., and Boyd M.R. Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Res. 48 (1988) 4827-4833
9. Tsukatani T., Suenaga H., Higuchi T., Akao T., Ishiyama M., Ezoe K., and Matsumoto K. Colorimetric cell proliferation assay for microorganisms in microtiter plate using water-soluble tetrazolium salts. J. Microbiol. Methods 75 (2008) 109-116
10. Deegan L.H., Cotter P.D., Hill C., and Ross P. Bacteriocins: biological tools for bio-preservation and shelf-life extension. Intl. Dairy J. 16 (2006) 1058-1071
11. Wang F., Cao L.T., and Hu S.H. A rapid and accurate 3-(4, 5-dimethyl thiazol-2-yl)-2-5-diphenyl tetrazolium bromide colorimetric assay for the quantification of bacteriocins with nisin as an example. J. Zhejiang Univ. Sci. B 8 (2007) 549-554
12. Mayr-Harting A., Hedges A.J., and Berkeley R.C.W. Methods for studying bacteriocins. Methods Microbiol. A 7 (1972) 315-422
13. Fujita K., Ichimasa S., Zendo T., Koga S., Yoneyama F., Nakayama J., and Sonomoto K. Structural analysis and characterization of lacticin Q, a novel bacteriocin belonging to a new family of unmodified bacteriocins of gram-positive bacteria. Appl. Environ. Microbiol. 73 (2007) 2871-2877