A chromatographic method for determination of supercoiled plasmid DNA concentration in complex solutions

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 877, Issue 24, 2009, Pages 2530-2536

  Bennemo, Mia ^a   Blom, Hans ^a   Emilsson, Anna ^a   Lemmens, Raf ^a  

^a GE Healthcare Bio Sciences AB   (Sweden)

Author keywords

Analytical group separation; Concentration determination; Supercoiled plasmid DNA; Thiophilic aromatic chromatography

Indexed keywords

A-COEFFICIENT; AGAROSE GEL ELECTROPHORESIS; ANALYTICAL GROUP SEPARATION; CAPILLARY GEL ELECTROPHORESIS; CHROMATOGRAPHIC METHODS; COMBINED METHOD; COMPLEX SOLUTION; CONCENTRATION DETERMINATION; GROUP SEPARATION; ISOFORMS; LIMIT OF DETECTION; MANUFACTURING PROCESS; PLASMID DNA; PRECISE ANALYSIS; SALT GRADIENT; STOCK SOLUTIONS; SUPERCOILED; SUPERCOILED PLASMID DNA; THIOPHILIC; THIOPHILIC AROMATIC CHROMATOGRAPHY;

AROMATIC COMPOUNDS; BIOCHEMICAL ENGINEERING; CAPILLARY ELECTROPHORESIS; CHROMATOGRAPHIC ANALYSIS; CONCENTRATION (PROCESS); DNA; ELECTROCHEMISTRY; GELATION; GELS; GENES; INDUSTRIAL ENGINEERING; NUCLEIC ACIDS; PRODUCTION ENGINEERING; RNA;

SIZE EXCLUSION CHROMATOGRAPHY;

PLASMID DNA; RNA; SODIUM CHLORIDE;

AGAR GEL ELECTROPHORESIS; ARTICLE; BACTERIAL STRAIN; CHROMATOGRAPHY; DNA SUPERCOILING; FERMENTATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PRIORITY JOURNAL; THIOPHILIC AROMATIC CHROMATOGRAPHY;

CHROMATOGRAPHY, LIQUID; DNA, SUPERHELICAL; ESCHERICHIA COLI; FERMENTATION; PLASMIDS;

EID: 67651021245     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jchromb.2009.06.037     Document Type: Article

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