A homogeneous cell-based bicistronic fluorescence assay for high-throughput identification of drugs that perturb viral gene recoding and read-through of nonsense stop codons

RNA

Volumn 15, Issue 8, 2009, Pages 1614-1621

  Cardno, Tony S ^a   Poole, Elizabeth S ^a   Mathew, Suneeth F ^a   Graves, Ryan ^a   Tate, Warren P ^a  

^a UNIVERSITY OF OTAGO   (New Zealand)

Author keywords

Bicistronic fluorescence; High throughput screening; Nonsense codon mutations; Recoding assay

Indexed keywords

LUCIFERASE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; FLUORESCENCE ANALYSIS; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; NONHUMAN; NONSENSE MUTATION; PRIORITY JOURNAL; STOP CODON; VIRUS GENE;

ANIMALS; BASE SEQUENCE; CELL LINE; CERCOPITHECUS AETHIOPS; CODON, NONSENSE; COS CELLS; FLUORESCENT DYES; FRAMESHIFTING, RIBOSOMAL; GENES; GENES, REPORTER; GENES, VIRAL; GREEN FLUORESCENT PROTEINS; HIV-1; HUMANS; LUMINESCENT PROTEINS; MICROBIAL SENSITIVITY TESTS; RNA, VIRAL; SPECTROMETRY, FLUORESCENCE;

HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 67650999738     PISSN: 13558382     EISSN: 14699001     Source Type: Journal    
DOI: 10.1261/rna.1586709     Document Type: Article

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