Hsp70 is required for optimal cell proliferation in mouse A6 mesoangioblast stem cells

Biochemical Journal

Volumn 421, Issue 2, 2009, Pages 193-200

  Turturici, Giuseppina ^a   Geraci, Fabiana ^a   Candela, Maria E ^a   Cossu, Giulio ^b   Giudice, Giovanni ^a   Sconzo, Gabriella ^a  

^a UNIVERSITY OF PALERMO   (Italy)

^b SAN RAFFAELE HOSPITAL   (Italy)

Author keywords

70 kDa heat shock protein (Hsp70); Cell doubling time; Ku factor; Mouse mesoangioblast; Short hairpin RNA (shRNA)

Indexed keywords

70 KDA HEAT SHOCK PROTEIN (HSP70); A6 CELLS; BASAL TRANSCRIPTION; BINDING PROTEINS; CELL CLONES; CONSENSUS SEQUENCE; HEAT SHOCK PROTEIN; HEAT STRESS; HEAT-SHOCK; HSP70 EXPRESSION; IN-VITRO; IN-VIVO; KU FACTOR; MOUSE MESOANGIOBLAST; SHORT HAIRPIN RNA (SHRNA); SPECIFICITY PROTEIN 1; STEM CELL; STRESS STIMULUS;

CELL PROLIFERATION; GENE TRANSFER; NUCLEIC ACIDS; PROTEINS; RNA; TRANSCRIPTION;

CELL MEMBRANES;

CIS ACTING ELEMENT; CONSTITUTIVE HSE BINDING FACTOR; HEAT SHOCK PROTEIN 70; KU ANTIGEN; MESSENGER RNA; NUCLEIC ACID BINDING PROTEIN; SHORT HAIRPIN RNA; TRANSCRIPTION FACTOR SP1; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; CELL CLONE; CELL CYCLE G0 PHASE; CELL CYCLE G1 PHASE; CELL PROLIFERATION; CELL STRESS; CONSENSUS SEQUENCE; CONTROLLED STUDY; GENE EXPRESSION REGULATION; HEAT SHOCK RESPONSE; MESOANGIOBLAST STEM CELL; MOUSE; NONHUMAN; OXYGEN TENSION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DNA BINDING; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN SYNTHESIS; RNA INTERFERENCE; STEM CELL; TRANSCRIPTION REGULATION;

ANIMALS; BINDING SITES; BLOOD VESSELS; CELL PROLIFERATION; FLOW CYTOMETRY; HSP70 HEAT-SHOCK PROTEINS; MICE; PROMOTER REGIONS, GENETIC; RNA INTERFERENCE; STEM CELLS; TRANSCRIPTION FACTORS;

EID: 67650904757     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20082309     Document Type: Article

References (38)

1. Mayer, M. P. and Bukau, B. (2005) Hsp70 chaperones: cellular functions and molecular mechanism. Cell. Mol. Life Sci. 62, 670-684
2. Sarge, K. D. and Cullen, K. E. (1997) Regulation of hsp expression during rodent spermatogenesis. Cell. Mol. Life Sci. 53, 191-197
3. 2/M cell cycle arrest. Cell Stress Chaperones 6, 326-336
4. Pelham, H. R. (1982) A regulatory upstream promoter element in the Drosophila hsp 70 heat-shock gene. Cell 30, 517-528
5. Wu, C., Zimarino, V., Tsai, C., Walker, B. and Wilson, S. (1990) Stress Proteins in Biology and Medicine (R. I., Morimoto, A. Tissieres and C. Georgopoulos, eds), pp. 429-442, Cold Spring Harbor Laboratory, Plainview, NY
6. Mathur, S. K., Sistonen, L., Brown, I. R., Murphy, S. P., Sarge, K. D. and Morimoto, R. I. (1994) Deficient induction of human hsp70 heat shock gene transcription in Y79 retinoblastoma cells despite activation of heat shock factor 1. Proc. Natl. Acad. Sci. U.S.A. 91, 8695-8699
7. Kingston, R. E., Schuetz, T. J. and Larin, Z. (1987) Heat-inducible human factor that binds to a human hsp70 promoter. Mol. Cell. Biol. 7, 1530-1534
8. Morimoto, R. I. (1993) Cells in stress: transcriptional activation of heat shock genes. Science 259, 1409-1410
9. Mosser, D. D., Theodorakis, N. G. and Morimoto, R. I. (1988) Coordinate changes in heat shock element-binding activity and HSP70 gene transcription rates in human cells. Mol. Cell. Biol. 8, 4736-4744
10. Hensold, J. O., Hunt, C. R., Calderwood, S. K., Housman, D. E. and Kingston, R. E. (1990) DNA binding of heat shock factor to the heat shock element is insufficient for transcriptional activation in murine erythroleukemia cells. Mol. Cell. Biol. 10, 1600-1608
11. Mosser, D. D., Kotzbauer, P. T., Sarge, K. D. and Morimoto, R. I. (1990) In vitro activation of heat shock transcription factor DNA-binding by calcium and biochemical conditions that affect protein conformation. Proc. Natl. Acad. Sci. U.S.A. 87, 3748-3752
12. Kim, D., Ouyang, H., Yang, S. H., Nussenzweig, A., Burgman, P. and Li, G. C. (1995) A constitutive heat shock element-binding factor is immunologically identical to the Ku autoantigen. J. Biol. Chem. 270, 15277-15284
13. Yang, S. H., Nussenzweig, A., Li, L., Kim, D., Ouyang, H. P. and Burgman, C. G. (1996) Modulation of thermal induction of hsp70 expression by Ku autoantigen or its individual subunits. Mol. Cell. Biol. 16, 3799-3806
14. Mimori, T., Akizu, M., Yamagata, H., Irada, S., Yoshida, S. and Homma, M. (1981) Characterization of a high molecular weight acidic nuclear protein recognized by autoantibodies from patients with polymyositis-schleroderma overlap. J. Clin. Invest. 68, 611-620
15. Tuteja, R. and Tuteja, N. (2000) Ku autoantigen: a multifunctional DNA-binding protein. Crit. Rev. Biochem. Mol. Biol. 35, 1-33
16. Liu, R. Y., Corry, P. M. and Lee, Y. J. (1994) Regulation of chemical stress-induced hsp70 gene expression in murine L929 cells. J. Cell Sci. 107, 2209-2214
17. Liu, R. Y., Kim, D., Yang, S. H. and Li, G. C. (1993) Dual control of heat shock response: involvement of a constitutive heat shock element-binding factor. Proc. Natl. Acad. Sci. U.S.A. 90, 3078-3082
18. Milarski, K. L. and Morimoto, R. I. (1986) Expression of human HSP70 during the synthetic phase of the cell cycle. Proc. Natl. Acad. Sci. U.S.A. 83, 9517-9521
19. Taira, T., Marita, T., Iguchi-Ariga, S. M. and Ariga, H. (1997) Replication of origin containing adenovirus DNA fragments that do not carry the terminal protein. Nucleic Acids Res. 25, 1975-1983
20. Suzuki, K. and Watanabe, M. (1994) Modulation of cell growth and mutation induction by introduction of the expression vector of human hsp70 gene. Exp. Cell Res. 215, 75-81
21. Wei, Y. Q., Zhao, X., Kariya, Y., Teshigawara, K. and Uchida, A. (1995) Inhibition of proliferation and induction of apoptosis by abrogation of heat-shock protein (HSP) 70 expression in tumor cells. Cancer Immunol. Immunother. 40, 73-78
22. Minasi, M. G., Riminucci, M., De Angelis, L., Borello, U., Berarducci, B., Innocenzi, A., Caprioli, A., Sirabella, D., Baiocchi, M., De Maria, R. et al. (2002) The mesoangioblast: a multipotent, self-renewing cell that originates from the aorta and differentiates into most mesodermal tissues. Development 129, 2773-2783
23. Cossu, G. and Bianco, P. (2003) Mesoangioblasts - vascular progenitors for extravascular mesodermal tissues. Curr. Opin. Genet. Dev. 13, 537-542
24. Geraci, F., Turturici, G., Galli, D., Cossu, G., Giudice, G. and Sconzo, G. (2006) Stress response in mesoangioblast stem cells. Cell Death Differ. 13, 1057-1063
25. Hunt, C. R., Gasser, D. L., Chaplin, D. D., Pierce, J. C. and Kozak, C. A. (1993) Chromosomal localization of five murine HSP70 gene family members: Hsp70-1, Hsp70-2, Hsp70-3, Hsc70t, and Grp78. Genomics 16, 193-198
26. Snoek, M., Jansen, M., Olavesen, M. G., Campbel, R. D., Teuscher, C. and van Vugt, H. (1993) Three Hsp70 genes are located in the C4-H-2D region: possible candidates for the Orch-1 locus. Genomics 17, 350-356
27. Dix, D. J., Garges, J. B. and Hong, R. L. (1998) Inhibition of hsp70-1 and hsp70-3 expression disrupts preimplantation embryogenesis and heightens embryo sensitivity to arsenic. Mol. Reprod. Dev. 51, 373-380
28. Wright, W. E. and Shay, J. W. (2006) Inexpensive low-oxygen incubators. Nat. Protocol. 1, 2088-2090
29. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254
30. Bevilacqua, A., Fiorenza, M. T. and Mangia, F. (1997) Developmental action of an episomic hsp70 gene promoter in two-cell mouse embryos by transcription factor Sp1. Nucleic Acids Res. 25, 1333-1338
31. Turturici, G., Geraci, F., Candela, M. E., Giudice, G., Gonzalez, F. and Sconzo, G. (2008) Hsp70 localizes differently from chaperone Hsc70 in mouse mesoangioblasts under physiological growth conditions. J. Mol. Histology 39, 571-578
32. Barnes, J. A., Dix, D. J., Collins, B. W., Luft, C. and Allen, J. W. (2001) Expression of inducible Hsp70 enhances the proliferation of MCF-7 breast cancer cells and protects against the cytotoxic effects of hyperthermia. Cell Stress Chaperones 6, 316-325
33. Merrill, G. F. (1998) Cell synchronization. Methods Cell Biol. 57, 229-249
34. Hunt, C. R., Dix, D. J., Sharma, G. G., Pandita, R. K., Gupta, A., Funk, M. and Pandita, T. K. (2004) Genomic instability and enhanced radiosensitivity in Hsp701- and Hsp70.3-deficient mice. Mol. Cell. Biol. 24, 899-911
35. Dix, D. J, Allen, J. W., Collins, B. W., Poorman-Allen, P., Mori, C., Blizard, D. R., Brown, P. R., Goulding, E. H., Strong, B. D. and Eddy, E. M. (1997) HSP70-2 is required for desynapsis of synaptonemal complexes during meiotic prophase in juvenile and adult mouse spermatocytes. Development 124, 4595-4603
36. Eddy, E. M. (1999) Role of heat shock protein HSP70-2 in spermatogenesis. Rev. Reprod. 4, 23-30
37. Sconzo, G., Palla, F., Agueli, C., Spinelli, G., Giudice, G., Cascino, D. and Geraci, F. (1999) Constitutive hsp70 is essential to mitosis during early cleavage of Paracentrotus lividus embryos: the blockage of constitutive hsp70 impairs mitosis. Biochem. Biophys. Res. Commun. 260, 143-149
38. Agueli, C., Geraci, F., Giudice, G., Chimenti, L., Cascino, D. and Sconzo, G. (2001) A constitutive 70 kDa heat-shock protein is localized on the fibres of spindles and asters at metaphase in an ATP-dependent manner: a new chaperone role is proposed. Biochem. J. 360, 413-419