Physiology of Aspergillus niger in oxygen-limited continuous cultures: Influence of aeration, carbon source concentration and dilution rate

Biotechnology and Bioengineering

Volumn 103, Issue 5, 2009, Pages 956-965

  Diano, A ^a   Peeters, J ^a   Dynesen, J ^b   Nielsen, J ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b NOVOZYMES A S   (Denmark)

Author keywords

Chemostat; Fungi; Organic acid production; Polyol production; Redox metabolism

Indexed keywords

ASPERGILLUS NIGER; CARBON CATABOLITE REPRESSION; CARBON SOURCE; CELLULAR RESPONSE; CONTINUOUS CULTURE; DILUTION RATE; FILAMENTOUS FUNGI; INDUSTRIAL PRODUCTION; INTRACELLULAR PH; LOW OXYGEN; MANNITOL PRODUCTION; MITOCHONDRIAL RESPIRATORY CHAIN; NIGER; ORGANIC ACID PRODUCTION; OSMOTIC PRESSURE; POLYOL PRODUCTION; POLYOLS; RE-OXIDATION; REDOX METABOLISM; SPECIFIC GROWTH RATE; TRICARBOXYLIC ACIDS;

BIOCHEMISTRY; CELL MEMBRANES; CHEMOSTATS; FUNGI; GLYCEROL; METABOLISM; ORGANIC ACIDS; OSMOSIS; PHYSIOLOGY;

OXYGEN SUPPLY;

ADENOSINE TRIPHOSPHATE; CARBOXYLIC ACID; ERYTHRITOL; GLUCOSE; GLYCEROL; MANNITOL; NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE;

AERATION; ARTICLE; ASPERGILLUS NIGER; CARBON SOURCE; CELL METABOLISM; CELL PH; CITRIC ACID CYCLE; CONCENTRATION RESPONSE; CONTINUOUS CULTURE; CONTROLLED STUDY; DILUTION; FUNGAL METABOLISM; FUNGAL PHENOMENA AND FUNCTIONS; GROWTH RATE; MITOCHONDRIAL RESPIRATION; NONHUMAN; OSMOSIS; OSMOTIC PRESSURE; OXIDATION; OXYGENATION; PHYSIOLOGY; RESPIRATORY CHAIN;

ADENOSINE TRIPHOSPHATE; ASPERGILLUS NIGER; CARBON; CULTURE MEDIA; ERYTHRITOL; GLYCEROL; INDUSTRIAL MICROBIOLOGY; MANNITOL; NAD; OXYGEN;

ASPERGILLUS NIGER; FUNGI;

EID: 67650799913     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22329     Document Type: Article

References (46)

1. Andersen MR, Nielsen ML, Nielsen J. 2008. Metabolic model integration of the bibliome, genome, metabolome and reactome of Aspergillus niger. Mol Syst Biol 4:178.
2. Barberel SI, Walker JRL. 2000. The effect of aeration upon the secondary metabolism of microorganisms. Biotechnol Genet Eng Rev 17:281-323.
3. Bocking SP, Wiebe MG, Robson GD, Christiansen LH, Trinci AP. 1999. Effect of branch frequency in Aspergillus oryzae on protein secretion and culture viscosity. Biotechnol Bioeng 65(6):638-648.
4. Carter BLA, Bull AT. 1971. The effect of oxygen tension in the medium on the morphology and growth kinetics of Aspergillus nidulans. J Gen Microbiol 65:265-273.
5. David H, Krogh AM, Roca C, Akesson M, Nielsen J. 2005. CreA influences the metabolic fluxes of Aspergillus nidulans during growth on glucose and xylose. Microbiology 151:2209-2221. (Pubitemid 40984294)
6. Diano A, Bekker-Jensen S, Dynesen J, Nielsen J. 2006. Polyol synthesis in Aspergillus niger: Influence of oxygen availability, carbon and nitrogen sources on the metabolism. Biotechnol Bioeng 94:899-908. (Pubitemid 44186897)
7. Dowzer CE, Kelly JM. 1991. Analysis of the creA gene, a regulator of carbon catabolite repression in Aspergillus nidulans. Mol Cell Biol 11:5701-5709.
8. Felenbok B, Kelly JM. 1996. Regulation of carbon metabolism in mycelia fungi. In: Brambl R, Marzluf GA, editors. The Mycota III. Biochemistry and Molecular Biology. Berlin: Springer-Verlag. p 369-380.
9. Gallmetzer M, Burgstaller W. 2002. Efflux of organic acids in Penicillium simplicissimum is an energy-spilling process, adjusting the catabolic carbon flow to the nutrient supply and the activity of catabolic pathways. Microbiology 148:1143-1149. (Pubitemid 34436788)
10. Haack MB, Olsson L, Hansen K, Eliasson LA. 2006. Change in hyphal morphology of Aspergillus oryzae during fed-batch cultivation. Appl Microbiol Biotechnol 70:482-487.
11. Hesse SJ, Ruijter GJ, Dijkema C, Visser J. 2002. Intracellular pH homeostasis in the filamentous fungus Aspergillus niger. Eur J Biochem 269:3485-3494. (Pubitemid 34988992)
12. Hossain M, Brooks JD, Maddox IS. 1984. The effect of the sugar source on citric acid production by Aspergillus niger. Appl Microbiol Biotechnol 19:393-397. (Pubitemid 14052995)
13. Ilyes H, Fekete E, Karaffa L, Fekete E, Sandor E, Szentirmai A, Kubicek CP. 2004. CreA-mediated carbon catabolite repression of beta-galactosidase formation in Aspergillus nidulans is growth rate dependent. FEMS Microbiol Lett 235:147-151. (Pubitemid 38668649)
14. Jernejc K, Legisa M. 2004. A drop of intracellular pH stimulates citric acid accumulation by some strains of Aspergillus niger. J Biotechnol 112: 289-297. (Pubitemid 39093961)
15. Karaffa L, Kubicek CP. 2003. Aspergillus niger citric acid accumulation: Do we understand this well working black box? Appl Microbiol Biotechnol 61:189-196.
16. Khan AH, Ghose TK. 1973. Kinetics of citric acid fermentation by Aspergillus niger. J Ferment Technol 51:734-741.
17. Kubicek CP, Zehentgruber O, El-Kalak H, Rohr M. 1980. Regulation of citric acid production by oxygen: Effect of dissolved oxygen tension on adenylate levels and respiration in Aspergillus niger. Eur J Appl Microbiol Biotechnol 9:101-115. (Pubitemid 10121638)
18. Kubicek-Pranz EM, Mozelt M, Rohr M, Kubicek CP. 1990. Changes in the concentration of fructose 2,6-bisphosphate in Aspergillus niger during stimulation of acidogenesis by elevated sucrose concentration. Biochim Biophys Acta 1033:250-255. (Pubitemid 20083723)
19. Legisa M, Grdadolnik SG. 2002. Influence of dissolved oxygen concentration on intracellular pH and consequently on growth rate of Aspergillus niger. Food Technol Biotechnol 40:27-32.
20. Legisa M, Kidric J. 1989. Initiation of citric acid accumulation in the early stages of Aspergillus niger growth. Appl Microbiol Biotechnol 31:453-457. (Pubitemid 19238020)
21. Magnuson JK, Lasure LL. 2004. Organic acid production by filamentous fungi. In: Tkacz JS, Lange L, editors. Advances in fungal biotechnology for industry, agriculture and medicine. New York: Kluwer Academic/ Plenum. p 307-340.
22. Mattey M, Bowes I. 1978. Citrate regulation of NADP+-specific isocitrate dehydrogenase of Aspergillus niger. Biochem Soc Trans 6:1224-1226.
23. Meijer S, Panagiotou G, Olsson L, Nielsen J. 2007. Physiological characterization of xylose metabolism in Aspergillus niger under oxygen-limited conditions. Biotechnol Bioeng 98(2):462-475. (Pubitemid 47518066)
24. Mogensen J, Nielsen HB, Hofmann G, Nielsen J. 2006. Transcription analysis using high-density micro-arrays of Aspergillus nidulans wild-type and creA mutant during growth on glucose or ethanol. Fungal Genet Biol 43:593-603.
25. Olsvik E, Tucker KG, Thomas CR, Kristiansen B. 1993. Correlation of Aspergillus niger broth rheological properties with biomass concentration and the shape of mycelial aggregates. Biotechnol Bioeng 42:1046-1052. (Pubitemid 23311899)
26. Papagianni M, Mattey M, Kristiansen B. 1999. The influence of glucose concentration on citric acid production and morphology of Aspergillus niger in batch and culture. Enzyme Microb Technol 25:710-717. (Pubitemid 29512408)
27. Pedersen H, Beyer M, Nielsen J. 2000a. Glucoamylase production in batch, chemostat and fedbatch cultivations by an industrial strain of Aspergillus niger. Appl Microbiol Biotechnol 53:272-277. (Pubitemid 30180117)
28. Pedersen H, Christensen B, Carsten H, Nielsen J. 2000b. Construction and characterisation of an oxalic acid nonproducing strain of Aspergillus niger. Metab Eng 2:34-41. (Pubitemid 30491365)
29. Peksel A, Torres NV, Liu J, Juneau G, Kubicek CP. 2002. 13C-NMR analysis of glucose metabolism during citric acid production by Aspergillus niger. Appl Microbiol Biotechnol 58:157-163. (Pubitemid 34118988)
30. Rahardjo YS, Sie S, Weber FJ, Tramper J, Rinzema A. 2005. Effect of low oxygen concentrations on growth and alpha-amylase production of Aspergillus oryzae in model solid-state fermentation systems. Biomol Eng 21:163-172. (Pubitemid 40313415)
31. Roels JA, Van den Berg J, Voncken RM. 1974. The rheology of mycelial broths. Biotechnol Bioeng 16:181-208.
32. Ruijter GJ, Bax M, Patel H, Flitter SJ, van de Vondervoort PJI, de Vries RP, van Kuyk PA, Visser J. 2003. Mannitol is required for stress tolerance in Aspergillus niger conidiospores. Eukaryot Cell 2:690-698. (Pubitemid 36996715)
33. Ruijter GJ, Panneman H, Visser J. 1997a. Overexpression of phosphofructokinase and pyruvate kinase in citric acid-producing Aspergillus niger. Biochim Biophys Acta 1334:317-326. (Pubitemid 27117536)
34. Ruijter GJ, Vanhanen SA, Gielkens MM, van de Vondervoort PJI, Visser J. 1997b. Isolation of Aspergillus niger creA mutants and effects of the mutations on expression of arabinases and L-arabinose catabolic enzymes. Microbiology 143(Pt 9):2991-2998. (Pubitemid 27437916)
35. Ruijter GJ, Visser J. 1997. Carbon repression in Aspergilli. FEMS Microbiol Lett 151:103-114.
36. Ruijter GJ, van de Vondervoort PJI, Visser J. 1999. Oxalic acid production by Aspergillus niger: An oxalate-non-producing mutant produces citric acid at pH 5 and in the presence of manganese. Microbiology 145(Pt 9):2569-2576. (Pubitemid 29454375)
37. Schrickx JM, Raedts MJH, Stouthamer AH, Vanverseveld HW. 1995. Organic acid production by Aspergillus niger in recycling culture analyzed by capillary electrophoresis. Anal Biochem 231(1):175-181.
38. Shroff RA, Lockington RA, Kelly JM. 1996. Analysis of mutations in the creA gene involved in carbon catabolite repression in Aspergillus nidulans. Can J Microbiol 42:950-959.
39. Shroff RA, O'Connor SM, Hynes MJ, Lockington RA, Kelly JM. 1997. Null alleles of creA, the regulator of carbon catabolite repression in Aspergillus nidulans. Fungal Genet Biol 22:28-38. (Pubitemid 27491343)
40. Singh M, Scrutton NS, Scrutton MC. 1988. NADPH generation in Aspergillus nidulans: Is the mannitol cycle involved? J Gen Microbiol 134:643-654.
41. Torres NV, Riol-Cimas JM, Wolshek M, Kubicek CP. 1996. Glucose transport by Aspergillus niger: The low-affinity carrier is only formed during growth on high glucose concentrations. Appl Microbiol Biotechnol 44:790-794.
42. van der Veen P, Ruijter GJG, Visser J. 1995. An extreme creA mutation in Aspergillus nidulans has severe effects on D-glucose utilization. Microbiology 141:2301-2306.
43. van Suijdam JC, Metz B. 1981. Influence of engineering variables upon the morphology of filamentous molds. Biotechnol Bioeng 23:111-148.
44. Witteveen CFB, Weber F, Busink R, Visser J. 1994. Isolation and characterization of two xylitol dehydrogenases from Aspergillus niger. Microbiology 140:1679-1685. (Pubitemid 24231156)
45. Wongwicharn A, McNeil B, Harvey LM. 1999. Effect of oxygen enrichment on morphology, growth, and heterologous protein production in chemostat cultures of Aspergillus niger B1-D. Biotechnol Bioeng 65(4):416-424. (Pubitemid 29482997)
46. Xu D, Madrid CP, Röhr M, Kubicek CP. 1989. The influence of type and concentration of carbon source on the production of citric acid by Aspergillus niger. Appl Microbiol Biotechnol 30:553-558.