Rapid diagnosis of candidaemia by real-time PCR detection of Candida DNA in blood samples

Journal of Medical Microbiology

Volumn 58, Issue 8, 2009, Pages 1106-1111

  Wellinghausen, Nele ^a,b   Siegel, Dunja ^a   Winter, Juliane ^a   Gebert, Susanne ^a  

^a UNIVERSITY HOSPITAL ULM   (Germany)

^b Dr Gaertner and Partner Laboratories   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; RIBOSOME RNA;

ADULT; AGED; ARTICLE; BLOOD CULTURE; BLOOD SAMPLING; CANDIDA; CANDIDEMIA; CHILD; CONTROLLED STUDY; DIAGNOSTIC PROCEDURE; DIAGNOSTIC VALUE; FUNGAL DETECTION; HEMATOLOGIC MALIGNANCY; HUMAN; INTENSIVE CARE UNIT; MAJOR CLINICAL STUDY; NUCLEOTIDE SEQUENCE; PRESCHOOL CHILD; PRIORITY JOURNAL; PROSPECTIVE STUDY; REAL TIME POLYMERASE CHAIN REACTION; SCHOOL CHILD;

ADOLESCENT; ADULT; AGED; CANDIDA; CHILD; CHILD, PRESCHOOL; DNA, FUNGAL; FEMALE; FUNGEMIA; HUMANS; INFANT; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION; RNA, FUNGAL; RNA, RIBOSOMAL, 18S; YOUNG ADULT;

EID: 67650747674     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/jmm.0.007906-0     Document Type: Article

References (17)

1. Ahmad, S., Khan, Z., Mustafa, A. S. & Khan, Z. U. (2002). Seminested PCR for diagnosis of candidemia: comparison with culture, antigen detection, and biochemical methods for species identification. J Clin Microbiol 40, 2483-2489. (Pubitemid 34747352)
2. Alam, F. F., Mustafa, A. S. & Khan, Z. U. (2007). Comparative evaluation of (1, 3)-b-D-glucan, mannan and anti-mannan antibodies, and Candida species-specific snPCR in patients with candidemia. BMC Infect Dis 7, 103-111.
3. Bone, R. C., Balk, R. A., Cerra, F. B., Dellinger, R. P., Fein, A. M., Knaus, W. A., Schein, R. M. & Sibbald, W. J. (1992). Definitions for sepsis and organ failure and guidelines for the use of innovative therapies in sepsis. The ACCP/SCCM Consensus Conference Committee. American College of Chest Physicians/Society of Critical Care Medicine. Chest 101, 1644-1655.
4. Bretagne, S. & Costa, J. M. (2006). Towards a nucleic acid-based diagnosis in clinical parasitology and mycology. Clin Chim Acta 363, 221-228. (Pubitemid 41745238)
5. Dunyach, C., Bertout, S., Phelipeau, C., Drakulovski, P., Reynes, J. & Mallie, M. (2008). Detection and identification of Candida spp. in human serum by LightCycler real-time polymerase chain reaction. Diagn Microbiol Infect Dis 60, 263-271. (Pubitemid 351253601)
6. Einsele, H., Hebart, H., Roller, G., Loffler, J., Rothenhofer, I., Muller, C. A., Bowden, R. A., van Burik, J., Engelhard, D. & other authors (1997). Detection and identification of fungal pathogens in blood by using molecular probes. J Clin Microbiol 35, 1353-1360.
7. Engel, C., Brunkhorst, F. M., Bone, H. G., Brunkhorst, R., Gerlach, H., Grond, S., Gruendling, M., Huhle, G., Jaschinski, U. & other authors (2007). Epidemiology of sepsis in Germany: results from a national prospective multicenter study. Intensive Care Med 33, 606-618. (Pubitemid 46474888)
8. Gebert, S., Siegel, D. & Wellinghausen, N. (2008). Rapid detection of pathogens in blood culture bottles by real-time PCR in conjunction with the pre-analytic tool MolYsis. J Infect 57, 307-316.
9. Horz, H. P., Scheer, S., Huenger, F., Vianna, M. E. & Conrads, G. (2008). Selective isolation of bacterial DNA from human clinical specimens. J Microbiol Methods 72, 98-102. (Pubitemid 350309683)
10. Innings, A., Ullberg, M., Johansson, A., Rubin, C. J., Noreus, N., Isaksson, M. & Herrmann, B. (2007). Multiplex real-time PCR targeting the RNase P RNA gene for detection and identification of Candida species in blood. J Clin Microbiol 45, 874-880. (Pubitemid 46427704)
11. Klingspor, L. & Jalal, S. (2006). Molecular detection and identification of Candida and Aspergillus spp. from clinical samples using real-time PCR. Clin Microbiol Infect 12, 745-753. (Pubitemid 43983799)
12. Kumar, A., Roberts, D., Wood, K. E., Light, B., Parrillo, J. E., Sharma, S., Suppes, R., Feinstein, D., Zanotti, S. & other authors (2006). Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock. Crit Care Med 34, 1589-1596. (Pubitemid 43810035)
13. Laupland, K. B., Kirkpatrick, A. W., Church, D. L., Ross, T. & Gregson, D. B. (2004). Intensive-care-unit-acquired bloodstream infections in a regional critically ill population. J Hosp Infect 58, 137-145. (Pubitemid 39317884)
14. Maaroufi, Y., Heymans, C., De Bruyne, J. M., Duchateau, V., Rodrigues-Villalobos, H., Aoun, M. & Crokaert, F. (2003). Rapid detection of Candida albicans in clinical blood samples by using a TaqMan-based PCR assay. J Clin Microbiol 41, 3293-3298. (Pubitemid 36842335)
15. Moreira-Oliveira, M. S., Mikami, Y., Miyaji, M., Imai, T., Schreiber, A. Z. & Moretti, M. L. (2005). Diagnosis of candidemia by polymerase chain reaction and blood culture: prospective study in a high-risk population and identification of variables associated with development of candidemia. Eur J Clin Microbiol Infect Dis 24, 721-726. (Pubitemid 41752067)
16. Tirodker, U. H., Nataro, J. P., Smith, S., Las Casas, L. & Fairchild, K. D. (2003). Detection of fungemia by polymerase chain reaction in critically ill neonates and children. J Perinatol 23, 117-122. (Pubitemid 36511738)
17. Wisplinghoff, H., Bischoff, T., Tallent, S. M., Seifert, H., Wenzel, R. P. & Edmond, M. B. (2004). Nosocomial bloodstream infections in US hospitals: analysis of 24,179 cases from a prospective nationwide surveillance study. Clin Infect Dis 39, 309-317. (Pubitemid 39050477)