Fluorescence emission and polarization for analyzing binding of ruthenium metalloglycocluster to lectin and tetanus toxin C-fragment

Bioconjugate Chemistry

Volumn 20, Issue 7, 2009, Pages 1296-1298

  Okada, Tomoko ^a   Makino, Taro ^a   Minoura, Norihiko ^a  

^a TOKYO UNIVERSITY OF TECHNOLOGY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CARBOHYDRATE; CONCANAVALIN A; GALACTOSE; GLUCOSE; LECTIN; PEANUT AGGLUTININ; RUTHENIUM COMPLEX; TETANUS TOXIN; WHEAT GERM AGGLUTININ;

ARTICLE; BINDING AFFINITY; BINDING SITE; CHEMICAL STRUCTURE; COMPLEX FORMATION; FLUORESCENCE; FLUORESCENCE EMISSION SPECTROSCOPY; FLUORESCENCE POLARIZATION; FLUORESCENCE SPECTROSCOPY; NONHUMAN; POLARIZATION; PROTEIN CARBOHYDRATE INTERACTION; RICINUS COMMUNIS; SYNTHESIS;

ARACHIS HYPOGAEA; RICINUS COMMUNIS; TRITICUM AESTIVUM;

EID: 67650674346     PISSN: 10431802     EISSN: None     Source Type: Journal    
DOI: 10.1021/bc900101u     Document Type: Article

References (10)

1. Bertozzi, C., and Kiessling, L. (2001) Chemical glycobiology. Science 291, 2357-2364.
2. Jelinek, R., and Kolusheva, S. (2004) Carbohydrate biosensors. Chem. Rev. 104, 5987-6015.
3. Hasegawa, T., Yonemura, T., Matsuura, K., and Kobayashi, K. (2003) Artificial metalloglycoclusters: Compact saccharide shell to induce high lectin affinity as well as strong luminescence. Bioconjugate Chem. 14, 728-737.
4. Kikkeri, R., García-Rubio, I., and Seeberger, P.-H. (2009) Ru(II)-carbohydrate dendrimers as photoinduced electron transfer lectin biosensors. Chem. Commun. 125, 235-237.
5. Gottschaldt, M., and Schubert, U. (2009) Prospects of metal complexes peripherally substituted with sugars in biomedicinal applications. Chem.-Eur. J. 15, 1548-1557.
6. Sakai, S., Shigemasa, Y., and Sasaki, T. (1999) Iron(II)-assisted assembly of trivalent GalNAc clusters and their interactions with GalNAc-specific lectins. Bull. Chem. Soc. Jpn. 72, 1313-1319.
7. See Figure S3 in Supporting Information.
8. Identification of Ru(bpy)2(bpy-2Gal) is in Supporting Information.
9. Emsley, P., Fotinou, C., Black, I., Fairweather, N., Charles, I., Watts, C., Hewitt, E., and Isaacs, N. (2000) The structures of the tetanus toxin c-fragment with carbohydrate subunit complexes provide insight into ganglioside binding. J. Biol. Chem. 275, 8889-8894.
10. Singh, A., Harrison, S., and Schoeniger, J. (2000) Gangliosides as receptors for biological toxins: Development of sensitive fluoroimmunoassays using ganglioside-bearing liposomes. Anal. Chem. 72, 6019-6024.