Rapid micropropagation of withania Somnifera L. accessions from axillary meristems

Journal of Herbs, Spices and Medicinal Plants

Volumn 13, Issue 4, 2008, Pages 123-133

  Sabir, Farzana ^a   Sangwan, Neelam S ^a   Chaurasiya, Narayan D ^a   Misra, Laxmi N ^a   Tuli, Rakesh ^b   Sangwan, Rajender S ^a  

^a CSIR CENTRAL INSTITUTE OF MEDICINAL AND AROMATIC PLANTS   (India)

^b CSIR NATIONAL BOTANICAL RESEARCH INSTITUTE   (India)

Author keywords

Ashwagandha; Indian ginseng; Medicinal plant; Solanaceae; Winter cherry; Withanolide

Indexed keywords

KINETIN; WITHANOLIDE;

GARDEN; MEDICINAL PLANT; SHOOT GROWTH; SHRUB; TEMPORAL PERIOD;

ACCLIMATIZATION; ARTICLE; DRUG ISOLATION; DRUG SYNTHESIS; GREENHOUSE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IN VITRO PROPAGATION; INDIA; LIQUID CHROMATOGRAPHY; MERISTEM; MICROPROPAGATION; MURASHIGE AND SKOOG MEDIUM; NONHUMAN; PLANT REGENERATION; PLANT ROOT; SHOOT; SHOOT GROWTH; SHOOT REGENERATION; SHRUB; SOIL; VERMICOMPOST; WITHANIA SOMNIFERA;

PANAX GINSENG; PHYSALIS ALKEKENGI; SOLANACEAE; WITHANIA SOMNIFERA;

EID: 67650269890     PISSN: 10496475     EISSN: 15403580     Source Type: Journal    
DOI: 10.1080/10496470801946166     Document Type: Article

References (19)

1. Anbalagan, K. and J. Sadique. 1981. Influence of an Indian medicine (Ashwagandha) on acute-phase reactants in inflammation. Indian J. Exp. Biol.19:245-249.
2. Baburaj, S. and K. Gunakeswaran. 1995. In vitro differentiation of shoots from leaf callus culture of Withania somnifera (L.) dunal. J. Indian Bot. Soc. 74:323-324.
3. Bhattacharya, S. K., R. K. Goel, R. Kaur, and S. Ghosal. 1987. Antistress activity of sitoindosides VII and VIII, new acylsterylglucosides from Withania somnifera. Phytotherapy Res. 1:32-39.
4. Devi, P. U., A. C. Sharada, F. E. Solomon, and M. S. Kamath. 1992. In vivo growth inhibitory effect of Withania somnifera (Ashwagandha) on a transplantable mouse tumor, Sarcoma 180. Indian J. Exp. Biol. 30:169-172.
5. Furmanowa, M., D. Gajdzis-Kuls, J. Ruszkowska, Z. Czarnocki, G. Obidoska, A. Sadowska, R. Rani, and S.N. Upadhyay. 2001. In vitro propagation of Withania somnifera and isolation of withanolides with immunosuppressive activity. Planta Med. 67:146-149.
6. Kulkarni, A., S. R. Thengane, and K. V. Krishnamurthy. 1996. Direct in vitro regeneration of leaf explant of Withania somnifera (L.) dunal. Plant Science 119:163-168.
7. Manickam, V. S., R.E. Mathavan, and R. Antonisamy. 2000. Regeneration of Indian ginseng plantlets from stem callus. Plant Cell, Tissue and Organ Culture 62:181-185.
8. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioas-says with tobacco tissue cultures. Physiol. Plant. 15:473-497
9. Rani, G., G. S. Virk, and A. Nagpal. 2003. Callus induction and plantlet regeneration in Withania somnifera (L.) dunal. In Vitro Cell Dev. Biol.-Plant 39:468-474.
10. Ray, S., and S. Jha. 2001. Production of withaferin A in shoot culture of Withania somnifera. Planta Med. 67:432-436.
11. Roja, G., and M. R. Heble. 1991. Tissue culture of Withania somnifera: Morphogenesis and withanolide synthesis. Phytotherapy Research 5:185-187.
12. Sabir, F., N. S. Sangwan, N. D. Chaurasiya, S. K. Mishra, P. Lal, L. N. Mishra, G. C. Uniyal, and R. S. Sangwan. 2005. Biochemical and phytochemical investigation on shoot differentiation induced by growth regulator kinetin and BAP, in Withania somnifera (L.) Dunal. In AK Kukreja, AK Mathur, S. Banerjee, A. Mathur, SPS Khanuja, and A. Sharma, eds. Proceedings of National Symposium on Plant Biotechnology: New Frontiers (2007), CIMAP, Lucknow. pp. 379-392.
13. th October, 2004, New Delhi.
14. Sangwan, R. S., N. D. Chaurasiya, L. N. Misra, P. Lal, G. C. Uniyal, R. Sharma, N.S. Sangwan, K.A. Suri, G. N. Qazi and R. Tuli. 2004. Phytochemical variability in commercial herbal products and preparations of Withania somnifera (Ashwagandha). Current Science 86:461-465.
15. Sangwan, R. S., N. D. Chaurasiya, L. N. Misra, P. Lal, G. C. Uniyal, R. Sharma, N.S. Sangwan, K.A. Suri, G.N. Qazi and R. Tuli. 2005. Process for isolation of withaferin-A from plant materials and products therefrom. US Patent (AppFT) 20050226950.
16. Sen, J., and A. K. Sharma. 1991. Micropropagation of Withania somnifera from germinating seeds and shoot tips. Plant Cell, Tissue and Organ Culture 26:71-73.
17. Singh, B., B. K. Chandan, and D.K. Gupta. 2003. Adaptogenic activity of a novel, withanolide free aqueous fraction from the roots of Withania somnifera Dun. Phytotherapy Research 17:531-536
18. Vitali, G., L. Conte, and M. Nicoletti. 1996. Withanolide composition and in vitro culture of Italian Withania somnifera. Planta Med. 62:287-288.
19. Kuboyama T, C. Tohda, and K. Komatsu. 2005. Neuritic regeneration and synaptic reconstruction induced by withanolide A. British J. Pharmacology 144:961-971.