Comparison of an in-house PCR assay, direct fluorescence assay and the Roche AMPLICOR Chlamydia trachomatis kit for detection of C. trachomatis

Journal of Medical Microbiology

Volumn 58, Issue 7, 2009, Pages 867-873

  Sachdeva, Poonam ^a   Patel, Achchhe Lal ^a   Sachdev, Divya ^a   Ali, Mashook ^a   Mittal, Aruna ^b   Saluja, Daman ^a  

^a UNIVERSITY OF DELHI   (India)

^b SAFDARJUNG HOSPITAL   (India)

Author keywords

[No Author keywords available]

Indexed keywords

ENDONUCLEASE; GENOMIC DNA; PHOSPHOLIPASE D;

ARTICLE; BACTERIUM DETECTION; CHLAMYDIA TRACHOMATIS; CHLAMYDIASIS; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; FEMALE; FLUORESCENCE ANALYSIS; GENE SEQUENCE; GENITAL TRACT INFECTION; HUMAN; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SEXUALLY TRANSMITTED DISEASE;

ADULT; CHLAMYDIA INFECTIONS; CHLAMYDIA TRACHOMATIS; DNA PRIMERS; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE, DIRECT; HUMANS; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; SENSITIVITY AND SPECIFICITY; TIME FACTORS;

EID: 67650165350     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/jmm.0.008698-0     Document Type: Article

References (36)

1. An, Q., Radcliffe, G., Vassallo, R., Buxton, D., O'Brien, W. J., Pelletier, D. A., Weisburg, W. G., Klinger, J. D. & Olive, D. M. (1992). Infection with a plasmid-free variant chlamydia related to Chlamydia trachomatis identified by using multiple assays for nucleic acid detection. J Clin Microbiol 30, 2814-2821.
2. Bass, C. A., Jungkind, D. L., Silverman, N. S. & Boodi, J. M. (1993). Clinical evaluation of a new polymerase chain reaction assay for detection of Chlamydia trachomatis in endocervical specimens. J Clin Microbiol 31, 2648-2653. (Pubitemid 23284896)
3. Black, C. M. (1997). Current methods of laboratory diagnosis of Chlamydia trachomatis infection. Clin Microbiol Rev 10, 160-184. (Pubitemid 27042835)
4. Black, C. M. (1998). "But Doctor, I'm Celibate!" The potential for, sources and implications of false-positive (and negative) results of tests for Chlamydia trachomatis. Clin Microbiol Newsl 20, 120-124.
5. Catry, M. A., Borrego, M. J., Cardoso, J., Azevedo, J. & Santo, I. (1995). Comparison of the Amplicor Chlamydia trachomatis test and cell culture for the detection of urogenital chlamydial infections. Genitourin Med 71, 247-250.
6. CDC (2002). Sexually transmitted diseases treatment guidelines 2002. Centers for Disease Control and Prevention. MMWR Recomm Rep 51 (RR-6), 1-78.
7. Chaudhry, U. & Saluja, D. (2002). Detection of Neisseria gonorrhoeae by PCR using orf1 gene as target. Sex Transm Infect 78, 72-78. (Pubitemid 34206322)
8. Chout, R., Vaton, S., Quist, D., Bucher, R., Leguyader-Desprees, P. & Sayada, C. (1995). Improvement of cervical Chlamydia detection in asymptomatic family planning attendees by using Amplicor Chlamydia trachomatis assay. Cell Mol Biol 41, 731-736.
9. Crotchfelt, K. A., Pare, B., Gaydos, C. & Quinn, T. C. (1998). Detection of Chlamydia trachomatis by the Gen-Probe AMPLIFIED Chlamydia trachomatis assay (AMP CT) in urine specimens from men and women and endocervical specimens from women. J Clin Microbiol 36, 391-394. (Pubitemid 28060301)
10. Divekar, A. A., Gogate, A. S., Shivkar, L. K., Gogate, S. & Badhwar, V. R. (2000). Disease prevalence in women attending the STD clinic in Mumbai (formerly Bombay), India. Int J STD AIDS 11, 45-48. (Pubitemid 30055136)
11. Farencena, A., Comanducci, M., Donati, M., Ratti, G. & Cevenini, R. (1997). Characterization of a new isolate of Chlamydia trachomatis which lacks the common plasmid and has properties of biovar trachoma. Infect Immun 65, 2965-2969. (Pubitemid 27264131)
12. Gaydos, C. A., Theodore, M., Dalesio, N., Wood, B. J. & Quinn, T. C. (2004). Comparison of three nucleic acid amplification tests for detection of Chlamydia trachomatis in urine specimens. J Clin Microbiol 42, 3041-3045. (Pubitemid 38938055)
13. George, J. A., Panchatcharam, T. S., Paramasivam, R., Balasubramanian, S., Chakrapani, V. & Murugan, G. (2003). Evaluation of diagnostic efficacy of PCR methods for Chlamydia trachomatis infection in genital and urine specimens of symptomatic men and women in India. Jpn J Infect Dis 56, 88-92. (Pubitemid 37161785)
14. Jaschek, G., Gaydos, C. A., Welsh, L. E. & Quinn, T. C. (1993). Direct detection of Chlamydia trachomatis in urine specimens from symptomatic and asymptomatic men by using a rapid polymerase chain reaction assay. J Clin Microbiol 31, 1209-1212. (Pubitemid 23129446)
15. Joshi, J. V., Playekar, S., Hazari, K. T., Shah, R. S. & Chitlange, S. M. (1994). The prevalence of Chlamydia trachomatis in young women. Natl Med J India 7, 57-59.
16. Kapur, S., Ahmed, M., Singh, V., Guido, K., Mittal, A. & Salhan, S. (2006). Fluorescent in situ hybridization can reliably detect rRNA sequences of Chlamydia trachomatis without amplification. Acta Cytol 50, 277-283. (Pubitemid 43837039)
17. Kessler, H. H., Pierer, K., Stuenzner, D., Aner-Grumbach, P., Haller, E. M. & Marth, E. (1994). Rapid detection of Chlamydia trachomatis in conjunctival, pharyngeal, and urethral specimens with a new polymerase chain reaction assay. Sex Transm Dis 21, 191-195. (Pubitemid 24310167)
18. Loeffelholz, M. J., Lewinski, C. A., Silver, S. R., Purohit, A. P., Herman, S. A., Buonagurio, D. A. & Dragon, E. A. (1992). Detection of Chlamydia trachomatis in endocervical specimens by polymerase chain reaction. J Clin Microbiol 30, 2847-2851.
19. Mahony, J. B. & Chernesky, M. A. (1985). Effect of swab type and storage temperature on the isolation of Chlamydia trachomatis from clinical specimens. J Clin Microbiol 22, 865-867. (Pubitemid 16217806)
20. Mahony, J. B., Luinstra, K. E., Sellors, J. W. & Chernesky, M. A. (1993). Comparison of plasmid- and chromosome-based polymerase chain reaction assays for detecting Chlamydia trachomatis nucleic acids. J Clin Microbiol 31, 1753-1758. (Pubitemid 23182570)
21. Mahony, J. B., Luinstra, K. E., Sellors, J. W., Pickard, L., Chong, S., Jang, D. & Chernesky, M. A. (1994). Role of confirmatory PCRs in determining performance of Chlamydia Amplicor PCR with endocervical specimens from women with a low prevalence of infection. J Clin Microbiol 32, 2490-2493. (Pubitemid 24284651)
22. Mårdh, P. A., Weströ m, L., Colleen, S. & Wølner-Hanssen, P. (1981). Sampling, specimen handling, and isolation techniques in the diagnosis of chlamydial and other genital infections. Sex Transm Dis 8, 280-285.
23. Niederhauser, C. & Kaempf, L. (2003). Improved sensitivity of the Chlamydia trachomatis Cobas Amplicor assay using an optimized procedure for preparation of specimens. Eur J Clin Microbiol Infect Dis 22, 118-121. (Pubitemid 36349976)
24. Ostergaard, L. (2002). Microbiological aspects of the diagnosis of Chlamydia trachomatis. Best Pract Res Clin Obstet Gynaecol 16, 789-799.
25. Palmer, L. & Falkow, S. A. (1986). Common plasmid of Chlamydia trachomatis. Plasmid 16, 52-62.
26. Schachter, J., Stamm, W. E. & Quinn, T. C. (1996). Discrepant analysis and screening for Chlamydia trachomatis. Lancet 348, 1308-1309. (Pubitemid 26377246)
27. Semeniuk, H., Zentner, A., Read, R. & Church, D. (2002). Evaluation of sequential testing strategies using non-amplified and amplified methods for detection of Chlamydia trachomatis in endocervical and urine specimens from women. Diagn Microbiol Infect Dis 42, 43-51. (Pubitemid 34124098)
28. Shattock, R. M., Patrizio, C., Simmonds, P. & Sutherland, S. (1998). Detection of Chlamydia trachomatis in genital swabs: comparison of commercial and in house amplification methods with culture. Sex Transm Infect 74, 289-293. (Pubitemid 28415208)
29. Singh, V., Rastogi, S., Garg, S., Kapur, S., Kumar, A., Salhan, S. & Mittal, A. (2002). Polymerase chain reaction for detection of endocervical Chlamydia trachomatis infection in Indian women attending a gynecology outpatient department. Acta Cytol 46, 540-544. (Pubitemid 34526125)
30. Singh, V., Salhan, S., Das, B. C. & Mittal, A. (2003). Predominance of Chlamydia trachomatis serovars associated with urogenital infections in females in New Delhi, India. J Clin Microbiol 41, 2700-2702. (Pubitemid 36712496)
31. Smith, I. W., Morrison, C. L., Patrizio, C. & McMillan, A. (1993). Use of a commercial PCR kit for detecting Chlamydia trachomatis. J Clin Pathol 46, 822-825. (Pubitemid 23287343)
32. Sriprakash, K. S. & Macavoy, E. S. (1987). Characterization and sequence of a plasmid from the trachoma biovar of Chlamydia trachomatis. Plasmid 18, 205-214. (Pubitemid 18047145)
33. Thejls, H., Gnarpe, J., Gnarpe, H., Larsson, G., Platz-Christensen, J., Ostergaard, L. & Victor, A. (1994). Expanded gold standard in the diagnosis of Chlamydia trachomatis in a low prevalence population: diagnostic efficacy of tissue culture, direct immunofluorescence, enzyme immunoassay, PCR and serology. Genitourin Med 70, 300-303. (Pubitemid 24316810)
34. Thomas, B. J., MacLeod, E. J. & Taylor-Robinson, D. (1993). Evaluation of sensitivity of 10 diagnostic assays for Chlamydia trachomatis by use of a simple laboratory procedure. J Clin Pathol 46, 912-914. (Pubitemid 23317670)
35. Wu, C. H., Lee, M. F., Yin, S. C., Yang, D. M. & Cheng, S. F. (1992). Comparison of polymerase chain reaction, monoclonal antibody based enzyme immunoassay, and cell culture for detection of Chlamydia trachomatis in genital specimens. Sex Transm Dis 19, 193-197.
36. Wylie, J. L., Moses, S., Babcock, R., Jolly, A., Giercke, S. & Hammond, G. (1998). Comparative evaluation of Chlamydiazyme, PACE 2, and AMPCT assays for detection of Chlamydia trachomatis in endocervical specimens. J Clin Microbiol 36, 3488-3491. (Pubitemid 28533215)