Refolding and purification of the human secreted group IID phospholipase A2 expressed as inclusion bodies in Escherichia coli

Protein Expression and Purification

Volumn 67, Issue 2, 2009, Pages 82-87

  Fonseca, Raquel Gomes ^a   Ferreira, Tatiana Lopes ^a   Ward, Richard J ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

hsPLA2 IID; Inclusion body; Phospholipid hydrolysis; Refolding

Indexed keywords

GUANIDINE; PHOSPHOLIPASE A2; PHOSPHOLIPID; RECOMBINANT PROTEIN;

ARTICLE; CELL INCLUSION; CHEMISTRY; CIRCULAR DICHROISM; ENZYMOLOGY; ESCHERICHIA COLI; GEL CHROMATOGRAPHY; GENETICS; HUMAN; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; PROTEIN ENGINEERING; PROTEIN FOLDING;

CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; CIRCULAR DICHROISM; ESCHERICHIA COLI; GUANIDINE; HUMANS; INCLUSION BODIES; PHOSPHOLIPASES A2; PHOSPHOLIPIDS; PROTEIN ENGINEERING; PROTEIN FOLDING; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 67650140709     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.04.006     Document Type: Article

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