Multiplex and quantifiable detection of nucleic acid from pathogenic fungi using padlock probes, generic real time PCR and specific suspension array readout

Journal of Microbiological Methods

Volumn 78, Issue 2, 2009, Pages 195-202

  Eriksson, Ronnie ^a   Jobs, Magnus ^a,b   Ekstrand, Charlotta ^a   Ullberg, Måns ^c   Herrmann, Björn ^a   Landegren, Ulf ^a   Nilsson, Mats ^a   Blomberg, Jonas ^a  

^a UPPSALA UNIVERSITY   (Sweden)

^b DALARNA UNIVERSITY   (Sweden)

^c KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

Multiplex detection; Nucleic acid hybridization; Padlock probe; Pathogenic fungi; Real time PCR; Rolling circle amplification; Suspension array

Indexed keywords

CIRCULAR DNA; MICROSPHERE; NUCLEIC ACID;

ARTICLE; CONTROLLED STUDY; DNA HYBRIDIZATION; DNA PROBE; DNA SEQUENCE; FUNGAL STRAIN; GENE AMPLIFICATION; GENE TARGETING; MICROORGANISM DETECTION; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEIC ACID ANALYSIS; NUCLEIC ACID HYBRIDIZATION; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; SIGNAL NOISE RATIO;

COLONY COUNT, MICROBIAL; DNA, FUNGAL; FUNGI; HUMANS; MYCOSES; OLIGONUCLEOTIDE PROBES; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

FUNGI;

EID: 67650117740     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2009.05.016     Document Type: Article

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