Comparison of two multiple-locus variable-number tandem-repeat analysis methods for molecular strain typing of human Brucella melitensis isolates from the Middle East

Journal of Clinical Microbiology

Volumn 47, Issue 7, 2009, Pages 2226-2231

  Tiller, Rebekah V ^a   De, Barun K ^a   Boshra, Marie ^b   Huynh, Lynn Y ^c   Van Ert, Matthew N ^c   Wagner, David M ^c   Klena, John ^b   Mohsen, T S ^d   El Shafie, S S ^d   Keim, Paul ^c   Hoffmaster, Alex R ^a   Wilkins, Patricia P ^a   Pimentel, Guillermo ^b  

^a CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

^b US Naval Medical Research Unit No 3 Egypt   (Egypt)

^c NORTHERN ARIZONA UNIVERSITY   (United States)

^d HAMAD MEDICAL CORPORATION   (Qatar)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL STRAIN; BACTERIUM ISOLATION; BRUCELLA MELITENSIS; BRUCELLOSIS; CAPILLARY ELECTROPHORESIS; CONTROLLED STUDY; GENE LOCUS; GENETIC VARIABILITY; GENOTYPE; INTERMETHOD COMPARISON; MICROBIAL DIVERSITY; MIDDLE EAST; MOLECULAR TYPING; NONHUMAN; PRIORITY JOURNAL; TANDEM REPEAT;

BACTERIAL TYPING TECHNIQUES; BRUCELLA MELITENSIS; BRUCELLOSIS; CLUSTER ANALYSIS; DNA FINGERPRINTING; EPIDEMIOLOGY, MOLECULAR; HUMANS; MIDDLE EAST; MINISATELLITE REPEATS; SENSITIVITY AND SPECIFICITY;

BRUCELLA; BRUCELLA MELITENSIS;

EID: 67650090504     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.02362-08     Document Type: Article

References (30)

1. Afifi, S., K. Earhart, M. A. Azab, F. G. Youssef, H. El Sakka, M. Wasfy, H. Mansour, S. El Oun, M. Rakha, and F. Mahoney 2005. Hospital-based surveillance for acute febrile illness in Egypt: a focus on community-acquired bloodstream infections. Am. J. Trop. Med. Hyg. 73:392-399.
2. Afifi, S., M. O. Wasfy, M. A. Azab, F. G. Youssef, G. Pimentel, T. W. Graham, H. Mansour, N. Elsayed, K. Earhart, R. Hajjeh, and F. Mahoney 2007. Laboratory-based surveillance of patients with bacterial meningitis in Egypt (1998-2004). Eur. J. Clin. Microbiol. Infect. Dis. 26:331-340.
3. Al Dahouk, S., P. L. Fleche, K. Nockler, I. Jacques, M. Grayon, H. C. Scholz, H. Tomaso, G. Vergnaud, and H. Neubauer 2007. Evaluation of Brucella MLVA typing for human brucellosis. J. Microbiol. Methods 69:137-145.
4. Alton, G. G., L. M. Jones, and D. E. Pietz 1975. Laboratory techniques in brucellosis. Monogr. Ser. World Health Organ. 1975:1-163.
5. Bikandi, J., R. San Millán, A. Rementeria, and J. Garaizar 2004. In silico analysis of complete bacterial genomes: PCR, AFLP-PCR and endonuclease restriction. Bioinformatics 20:798-799.
6. Bricker, B. J., and S. M. Halling 1994. Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J. Clin. Microbiol. 32:2660-2666.
7. Bricker, B. J., D. R. Ewalt, and S. M. Halling 2003. Brucella 'HOOF-Prints': strain typing by multi-locus analysis of variable number tandem repeats (VNTRs). BMC Microbiol. 3:15.
8. Cloeckaert, A., J. M. Verger, M. Grayon, and O. Grepinet 1995. Restriction site polymorphism of the genes encoding the major 25 kDa and 36 kDa outer-membrane proteins of Brucella. Microbiology 141:2111-2121.
9. DelVecchio, V. G., V. Kapatral, R. J. Redkar, G. Patra, C. Mujer, T. Los, N. Ivanova, I. Anderson, A. Bhattacharyya, A. Lykidis, G. Reznik, L. Jablonski, N. Larsen, M. D'Souza, A. Bernal, M. Mazur, E. Goltsman, E. Selkov, P. H. Elzer, S. Hagius, D. O'Callaghan, J. J. Letesson, R. Haselkorn, N. Kyrpides, and R. Overbeek 2002. The genome sequence of the facultative intracellular pathogen Brucella melitensis. Proc. Natl. Acad. Sci. USA 99:443-448.
10. Ficht, T. A., H. S. Husseinen, J. Derr, and S. W. Bearden 1996. Species-specific sequences at the omp2 locus of Brucella type strains. Int. J. Syst. Bacteriol. 46:329-331.
11. Foster, G., B. S. Osterman, J. Godfroid, I. Jacques, and A. Cloeckaert 2007. Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts. Int. J. Syst. Evol. Microbiol. 57:2688-2693.
12. Gándara, B., A. L. Merino, M. A. Rogel, and E. Martinez-Romero 2001. Limited genetic diversity of Brucella spp. J. Clin. Microbiol. 39:235-240.
13. Gee, J. E., B. K. De, P. N. Levett, A. M. Whitney, R. T. Novak, and T. Popovic 2004. Use of 16S rRNA gene sequencing for rapid confirmatory identification of Brucella isolates. J. Clin. Microbiol. 42:3649-3654.
14. Halling, S. M., B. D. Peterson-Burch, B. J. Bricker, R. L. Zuerner, Z. Qing, L. L. Li, V. Kapur, D. P. Alt, and S. C. Olsen 2005. Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis. J. Bacteriol. 187:2715-2726.
15. Hinić, V., I. Brodard, A. Thomann, Z. Cvetnic, P. V. Makaya, J. Frey, and C. Abril 2008. Novel identification and differentiation of Brucella melitensis, B. abortus, B. suis, B. ovis, B. canis, and B. neotomae suitable for both conventional and real-time PCR systems. J. Microbiol. Methods 75:375-378.
16. Huynh, L. Y., M. N. Van Ert, T. Hadfield, W. S. Probert, B. H. Bellaire, M. Dobson, R. J. Burgess, R. S. Weyant, T. Popovic, S. Zanecki, D. M. Wagner, and P. Keim 2008. Multiple locus variable number tandem repeat (VNTR) analysis (MLVA) of Brucella spp. identifies species specific markers and insights into phylogenetic relationships, p. 47-54. In V. St. Georgiev, K. A. Western, and J. J. McGowan (ed.), National Institute of Allergy and Infectious Disease, NIH, vol. 1. Frontiers in research. Humana Press, Totowa, NJ.
17. Kattar, M. M., R. F. Jaafar, G. F. Araj, P. Le Flèche, G. M. Matar, R. Abi Rached, S. Khalife, and G. Vergnaud 2008. Evaluation of a multilocus variable-number tandem-repeat analysis scheme for typing human Brucella isolates in a region of brucellosis endemicity. J. Clin. Microbiol. 46:3935-3940.
18. Le Flèche, P., I. Jacques, M. Grayon, S. Al Dahouk, P. Bouchon, F. Denoeud, K. Nockler, H. Neubauer, L. A. Guilloteau, and G. Vergnaud 2006. Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay. BMC Microbiol. 6:9.
19. Marianelli, C., F. Ciuchini, M. Tarantino, P. Pasquali, and R. Adone 2006. Molecular characterization of the rpoB gene in Brucella species: new potential molecular markers for genotyping. Microbes Infect. 8:860-865.
20. Mohamady, H., H. I. Shaheen, J. Klena, I. Nakhla, M. Weiner, and A. Armstrong 2007. Case report: traveler's Brucella-specific IgA and IgM antibodies as early serodiagnosis markers of infection. Am. J. Trop. Med. Hyg. 77(Suppl. 5):259.
21. Pappas, G., N. Akritidis, M. Bosilkovski, and E. Tsianos. Brucellosis 2005. N. Engl. J. Med. 352:2325-2336.
22. Pappas, G., P. Papadimitriou, N. Akritidis, L. Christou, and E. V. Tsianos 2006. The new global map of human brucellosis. Lancet Infect. Dis. 6:91-99.
23. Pappas, G., and Z. A. Memish 2007. Brucellosis in the Middle East: a persistent medical, socioeconomic and political issue. J. Chemother. 19:243-248.
24. Paulsen, I. T., R. Seshadri, K. E. Nelson, J. A. Eisen, J. F. Heidelberg, T. D. Read, R. J. Dodson, L. Umayam, L. M. Brinkac, M. J. Beanan, S. C. Daugherty, R. T. Deboy, A. S. Durkin, J. F. Kolonay, R. Madupu, W. C. Nelson, B. Ayodeji, M. Kraul, J. Shetty, J. Malek, S. E. Van Aken, S. Riedmuller, H. Tettelin, S. R. Gill, O. White, S. L. Salzberg, D. L. Hoover, L. E. Lindler, S. M. Halling, S. M. Boyle, and C. M. Fraser 2002. The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts. Proc. Natl. Acad. Sci. USA 99:13148-13153.
25. Probert, W. S., K. N. Schrader, N. Y. Khuong, S. L. Bystrom, and M. H. Graves 2004. Real-time multiplex PCR assay for detection of Brucella spp., B. abortus, and B. melitensis. J. Clin. Microbiol. 42:1290-1293.
26. Sánchez, D. O., R. O. Zandomeni, S. Cravero, R. E. Verdun, E. Pierrou, P. Faccio, G. Diaz, S. Lanzavecchia, F. Aguero, A. C. Frasch, S. G. Andersson, O. L. Rossetti, O. Grau, and R. A. Ugalde 2001. Gene discovery through genomic sequencing of Brucella abortus. Infect. Immun. 69:865-868.
27. Scholz, H. C., Z. Hubalek, I. Sedlacek, G. Vergnaud, H. Tomaso, S. Al Dahouk, F. Melzer, P. Kampfer, H. Neubauer, A. Cloeckaert, M. Maquart, M. S. Zygmunt, A. M. Whatmore, E. Falsen, P. Bahn, C. Gollner, M. Pfeffer, B. Huber, H. J. Busse, and K. Nockler 2008. Brucella microti sp. nov., isolated from the common vole Microtus arvalis. Int. J. Syst. Evol. Microbiol. 58:375-382.
28. Thimm, B., and W. Wundt 1976. The epidemiological situation of brucellosis in Africa. Dev. Biol. Stand. 31:201-217.
29. Whatmore, A. M., S. J. Shankster, L. L. Perrett, T. J. Murphy, S. D. Brew, R. E. Thirlwall, S. J. Cutler, and A. P. MacMillan 2006. Identification and characterization of variable-number tandem-repeat markers for typing of Brucella spp. J. Clin. Microbiol. 44:1982-1993.
30. Whatmore, A. M., L. L. Perrett, and A. P. MacMillan 2007. Characterisation of the genetic diversity of Brucella by multilocus sequencing. BMC Microbiol. 7:34.