Characterization and diagnostic tests of resistance to acetohydroxyacid synthase inhibitors due to an Asp376Glu substitution in Amaranthus powellii

Pesticide Biochemistry and Physiology

Volumn 95, Issue 1, 2009, Pages 38-46

  Ashigh, Jamshid ^a   Corbett, Cheryl Ann L ^b   Smith, Peter J ^b   Laplante, Julie ^b   Tardif, François J ^b  

^a NEW MEXICO STATE UNIVERSITY   (United States)

^b UNIVERSITY OF GUELPH   (Canada)

Author keywords

Acetolactate synthase; AHAS; ALS; Green pigweed; Herbicide resistance; PCR amplification of specific alleles; Powell amaranth; Restriction fragment length polymorphism

Indexed keywords

AMARANTHUS; AMARANTHUS CAUDATUS; AMARANTHUS HYBRIDUS; AMARANTHUS POWELLII;

EID: 67650076193     PISSN: 00483575     EISSN: 10959939     Source Type: Journal    
DOI: 10.1016/j.pestbp.2009.06.002     Document Type: Article

References (34)

1. Chipman D., Barak Z., and Schloss J.V. Biosynthesis of 2-aceto-2-hydroxy acids: acetolactate synthases and acetohydroxyacid synthases. Biochim. Biophys. Acta 1385 (1998) 401-419
2. Singh B.K. Biosynthesis of valine, leucine, and isoleucine. In: Singh B.K. (Ed). Plant Amino Acids: Biochemistry and Biotechnology (1999), Marcel Dekker Inc., Princeton, NJ 227-247
3. Shaner D.L., and Singh B.K. Acetohydroxyacid synthase inhibitors. In: Roe R.M., Burton J.D., and Kuhr R.J. (Eds). Herbicide Activity: Toxicology Biochemistry and Molecular Biology (1997), IOS Press, Amsterdam, The Netherlands 69-110
4. I. Heap, The International Survey of Herbicide Resistant Weeds. Available from: (verified July 24, 2008).
5. Duggleby R.G., and Pang S.S. Acetohydroxyacid synthase. J. Biochem. Mol. Biol. 33 (2000) 1-36
6. Devine M.D., and Eberlein C.V. Physiological, biochemical and molecular aspects of herbicide resistance based on altered target sites. In: Roe R.M., Burton J.D., and Kuhr R.J. (Eds). Herbicide Activity: Toxicology Biochemistry and Molecular Biology (1997), IOS Press, Amsterdam, The Netherlands 159-185
7. Chong C.K., and Choi J.D. Amino acid residues conferring herbicide tolerance in tobacco acetolactate synthase. Biochem. Biophys. Res. Commun. 279 (2000) 462-467
8. Guttieri M.J., Eberlein C.V., and Thill D.C. Diverse mutations in the acetolactate synthase gene confer chlorosulfuron resistance in kochia (Kochia scoparia) biotypes. Weed Sci. 43 (1995) 175-178
9. Bernasconi P., Woodworth A.R., Rosin B.A., Subramanian M.V., and Siehl D.L. A naturally occurring point mutation confers broad range tolerance to herbicides that target acetolactate synthase. J. Biol. Chem. 270 (1995) 17381-17385
10. Boutsalis P., Karotam J., and Powles S.B. Molecular basis of resistance to acetolactate synthase-inhibiting herbicides in Sisymbrium orientale and Brassica tournefortii. Pestic. Sci. 55 (1999) 507-516
11. Tranel P.J., and Wright T.R. Resistance of weeds to ALS inhibiting herbicides: what have we learned?. Weed Sci. 50 (2002) 700-712
12. Whaley C.M., Wilson H.P., and Westwood J.H. A new mutation in plant ALS confers resistance to five classes of ALS-inhibiting herbicides. Weed Sci. 55 (2007) 83-90
13. Eberlein C.V., Guttieri M.J., Mallory-Smith C.A., Thill D.C., and Baerg R.J. Altered acetolactate synthase activity in ALS-inhibitor resistant prickly lettuce (Lactuca serriola). Weed Sci. 45 (1997) 212-217
14. Mourad G., Williams D., and King J. A double mutant allele, csr1-4 of Arabidopsis thaliana encodes an acetolactate synthase with altered kinetics. Planta 196 (1995) 64-68
15. 205Val substitution in acetohydroxyacid synthase of Eastern black nightshade (Solanum ptychanthum) reduces sensitivity to herbicides and feedback inhibition. Weed Sci. 55 (2007) 558-565
16. Corbett C.A.L., and Tardif F.J. Detection of resistance to acetohydroxyacid synthase inhibitors in Amaranthus sp. using DNA polymorphisms. Pestic. Biochem. Physiol. 92 (2008) 48-55
17. Corbett C.A.L., and Tardif F.J. Detection of resistance to acetolactate synthase inhibitors in weeds with emphasis on DNA based techniques. Pest Manag. Sci. 62 (2006) 584-597
18. McNaughton K.E., Letarte J., Lee E.A., and Tardif F.J. Mutations in ALS confer herbicide resistance in redroot pigweed (Amaranthus retroflexus) and Powell amaranth (Amaranthus powellii). Weed Sci. 53 (2005) 17-22
19. Ferguson G.M., Hamill A.S., and Tardif F.J. ALS inhibitor resistance in populations of Powell amaranth and redroot pigweed. Weed Sci. 49 (2001) 448-453
20. Ray T.B. Site of action of chlorosulfuron: inhibition of valine and isoleucine biosynthesis in plants. Plant Physiol. 75 (1984) 827-831
21. Westerfelt W.W. A colorimetric determination of blood acetoin. J. Biol. Chem. 161 (1945) 495-502
22. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
23. Seefeldt D.L., Jensen J.E., and Fuerst E.P. Log-logistic analysis of herbicide dose-response relationships. Weed Technol. 9 (1995) 218-227
24. Diebold R.S., McNaughton K.E., Lee E.A., and Tardif F.J. Multiple resistance to imazethapyr and atrazine in Powell amaranth (Amaranthus powellii). Weed Sci. 51 (2003) 312-318
25. K.E. McNaughton, Mutations conferring resistance to ALS inhibiting herbicides in redroot pigweed (Amaranthus retroflexus L.) and green pigweed (Amaranthus powellii S. Wats.), M.Sc. Thesis, University of Guelph, Guelph, Ontario, Canada, 2001.
26. McCourt J.A., Pang S.S., King-Scott J., Guddat L.W., and Duggleby R.G. Herbicide-binding sites revealed in the structure of plant acetohydroxyacid synthase. PNAS 103 (2006) 569-573
27. Foes M.J., Liu L., Vigue G., Stoller E.W., Wax L.M., and Tranel P.J. A kochia (Kochia scoparia) biotype resistant to triazine and ALS-inhibiting herbicides. Weed Sci. 47 (1999) 20-27
28. Sprague C.L., Stoller E.W., Wax L.M., and Horak M.J. Palmer amaranth (Amaranthus palmeri) and common waterhemp (Amaranthus rudis) resistant to selected ALS-inhibiting herbicides. Weed Sci. 45 (1997) 192-197
29. C.A.L. Corbett, DNA-based diagnostic tests for the detection of acetolactate synthase-inhibiting herbicide resistance in Amaranthus sp., M.Sc. Thesis, University of Guelph, Guelph, Ontario, Canada, 2004.
30. Duggleby R.G., Pang S.S., Yu H., and Guddat L.W. Systematic characterization of mutations in yeast acetohydroxyacid synthase. Interpretation of herbicide-resistance data. Eur. J. Biochem. 270 (2003) 2895-2904
31. Preston C., Stone L.M., Rieger M.A., and Baker J. Multiple effects of a naturally occurring proline to threonine substitution within acetolactate synthase in two herbicide-resistant populations of Lactuca serriola. Pestic. Biochem. Physiol. 84 (2006) 227-235
32. Dyer W.E., Chee P.W., and Fay P.K. Rapid germination of sulfonylurea-resistant Kochia scoparia accessions is associated with elevated seed levels of branched chain amino acids. Weed Sci. 41 (1993) 18-22
33. Eberlein C.V., Guttieri M.J., Berger P.H., Fellman J.K., Mallory-Smith C.A., Thill D.C., Baerg R.J., and Belknap W.R. Physiological consequences of mutation for ALS-inhibitor resistance. Weed Sci. 47 (1999) 383-392
34. J. Ashigh, Resistance to acetohydroxyacid synthase-inhibiting herbicides in populations of Eastern black nightshade (Solanum ptycanthum Dun.) from Ontario, Ph.D. Thesis, University of Guelph, Guelph, Canada, 2006.