Molecular analysis of HPV 16 E6I/E6II spliced mRNAs and correlation with the viral physical state and the grade of the cervical lesion

Journal of Medical Virology

Volumn 81, Issue 7, 2009, Pages 1276-1282

  Cricca, Monica ^a   Venturoli, Simona ^a   Leo, Elisa ^a   Costa, Silvano ^a   Musiani, Monica ^a   Zerbini, Marialuisa ^a  

^a UNIVERSITY OF BOLOGNA   (Italy)

Author keywords

Cervical lesions; DNA physical state; HPV 16 E6*I II spliced transcripts

Indexed keywords

MESSENGER RNA; E6 PROTEIN, HUMAN PAPILLOMAVIRUS TYPE 16; ONCOPROTEIN; REPRESSOR PROTEIN; VIRUS RNA;

ARTICLE; CONTROLLED STUDY; CORRELATION ANALYSIS; DISEASE COURSE; FEMALE; GENETIC TRANSCRIPTION; HUMAN; HUMAN PAPILLOMAVIRUS TYPE 16; HUMAN TISSUE; MOLECULAR GENETICS; NONHUMAN; NUCLEOTIDE SEQUENCE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; UTERINE CERVIX CYTOLOGY; UTERINE CERVIX DISEASE; VIRUS DETECTION; VIRUS GENOME; VIRUS STRAIN; GENE EXPRESSION PROFILING; GENETICS; HOSPITALIZATION; PATHOLOGY; RNA SPLICING; UTERINE CERVIX TUMOR; VAGINA SMEAR; VIROLOGY;

HUMAN PAPILLOMAVIRUS TYPE 16;

FEMALE; GENE EXPRESSION PROFILING; HUMAN PAPILLOMAVIRUS 16; HUMANS; ONCOGENE PROTEINS, VIRAL; REPRESSOR PROTEINS; RNA SPLICING; RNA, MESSENGER; RNA, VIRAL; SEVERITY OF ILLNESS INDEX; TRANSCRIPTION, GENETIC; UTERINE CERVICAL NEOPLASMS; VAGINAL SMEARS;

EID: 67649957964     PISSN: 01466615     EISSN: 10969071     Source Type: Journal    
DOI: 10.1002/jmv.21496     Document Type: Article

References (23)

1. Andersson S, Safari H, Mints M, Lewensohn-Fuchs I, Gyllensten U, Johansson B. 2005. Type distribution, viral load and integration status of high risk human papillomaviruses in pre-stages of cervical cancer (CIN). Br J Cancer 92:2195-2200. (Pubitemid 40966257)
2. Arias-Pulido H, Peyton CL, Joste NE, Vargas H, Wheeler CM. 2006. Human papillomavirus type 16 integration in cervical carcinoma in situ and in invasive cervical cancer. J Clin Microbiol 44:1755-1762. (Pubitemid 43726265)
3. Bosch FX, Lorincz A, Munõz N, Meijer CJLM, Shah KV. 2002. The causal relation between human papillomavirus and cervical cancer. J Clin Pathol 55:244-265. (Pubitemid 34293391)
4. Cornelissen MT, Smits HL, Briët MA, Tweel JG, Struyk APHB, Noorda J, Schegget J. 1990. Uniformity of the splicing pattern of the E6/E7 transcripts in human papillomavirus type 16-transformed human fibroblasts, human cervical premalignant lesions and carcinomas. J Gen Virol 71:1243-1246. (Pubitemid 20174368)
5. Cricca M, Morselli-Labate AM, Venturoli S, Ambretti S, Gentilomi GA, Gallinella G, Costa S, MusianiM, ZerbiniM. 2007. Viral DNA load, physical status and E2/E6 ratio as markers to grade HPV 16 positive women for high-grade cervical lesions. Gynecol Oncol 106:549-557. (Pubitemid 47302471)
6. Falcinelli C, Claas E, Kleter B, QuintWG.1992. Detection of thehuman papilloma virus type 16 mRNA-transcripts in cytological abnormal scrapings. J Med Virol 37:93-98.
7. Godfrey TE, Kim SH, Chavira M, Ruff DW, Warren RS, Gray JW, Jensen RH. 2000. Quantitative mRNA expression analysis from formalin-fixed, paraffin-embedded tissues using 5′ nuclease quantitative reverse transcription-polymerase chain reaction. J Mol Diagn 2:84-91.
8. Gravitt PE, Peyton C, Wheeler C, Apple R, Higuchi R, Shah KV. 2003. Reproducibility of HPV16 and HPV18 viral load quantitation using TaqMan real-time PCR assays. J Virol Methods 112:23-33. (Pubitemid 37338255)
9. Jeon S, Lambert P. 1995. Integration of human papillomavirus type 16 DNA into the human genome leads to increased stability of E6 and E7mRNAs: Implications for cervical carcinogenesis. Proc Natl Acad Sci USA 92:1654-1658.
10. Johnson MA, Blomfield PI, Bevan IS, Woodman CB, Young LS. 1990. Analysis of human papillomavirus type 16 E6-E7 transcription in cervical carcinomas and normal cervical epithelium using the polymerase chain reaction. J Gen Virol 71:1473-1479. (Pubitemid 20225847)
11. KalantariM, Karlsen F, Kristensen G, Holm R, Hagmar B, Johansson B. 1998. Disruption of the E1 and E2 reading frames of HPV 16 in cervical carcinoma is associated with poor prognosis. Int J Gynecol Pathol 17:146-153. (Pubitemid 28151014)
12. Kösel S, Burggraf S, Engelhardt W, Olgemöller B. 2007. Increased levels of HPV 16 E6*I transcripts in high-grade cervical cytology and histology (CIN II+) detected by rapid real-time RT-PCR amplification. Cytopathology 18:290-299. (Pubitemid 47476152)
13. Kulmala SMA, Syrjänen SM, Gyllesten UB. 2006. Early integration of high copy HPV 16 detectable in women with normal and low grade cervical cytology and histology. J Clin Pathol 59:513-517.
14. Lukaszuk K, Liss J, Wozniak I, Emerich J, Wójcikowski C. 2003. Human papillomavirus type 16 status in cervical carcinoma cell DNA assayed by multiplex PCR. J Clin Microbiol 41:608-612. (Pubitemid 36184169)
15. McNicol P, Guijon F, Wayne S, Hidajat R, Paraskevas M. 1995. Expression of human papillomavirus type 16 E6-E7 open reading frame varies quantitatively in biopsy tissue from different grades of cervical intraepithelial neoplasia. J Clin Microbiol 33:1169-1173.
16. Münger K, Werness BA, Dyson N, Phelps WC, Harlow E, Howley PM. 1989. Complex formation of human papillomavirus E7 proteins with the retinoblastoma tumor suppressor gene product. EMBO J 8:4099-4105.
17. Scheffner M, Werness BA, Huibregtse JM, Levine AJ, Howley PM. 1990. The E6 oncoprotein encoded by human papillomavirus types 16 and 18 promotes the degradation of p53. Cell 63:1129-1136. (Pubitemid 120035062)
18. Sedman SA, Barbosa MS, Vass WC, Hubbert NL, Haas JA, Lowy DR, Schiller JT. 1991. The full-length E6 protein of human papillomavirus type 16 has transforming and trans-activating activities and cooperates with E7 to immortalize keratinocytes in culture. J Virol 65:4860-4866.
19. Smotkin D, Prokoph H, Wettstein FO. 1989. Oncogenic and nononcogenic human genital papillomaviruses generate the E7 mRNA by different mechanisms. J Virol 63:1441-1447. (Pubitemid 19061390)
20. Sotlar K, Selinka HC, Menton M, Kandolf R, Bültmann B. 1998. Detection of human papillomavirus type 16 E6/E7 oncogene transcripts in dysplastic and nondysplastic cervical scrapes by nested RT-PCR. Gynecol Oncol 69:114-121. (Pubitemid 28246996)
21. Stacey SN, Jordan D, Snijders PJ,Mackett M, Walboomers JM,Arrand JR. 1995. Translation of the human papillomavirus type 16 E7 oncoprotein from bicistronic mRNA is independent of splicing events within the E6 open reading frame. J Virol 69:7023-7031.
22. Zerbini M, Venturoli S, Cricca M, Gallinella G, De Simone P, Costa S, Santini D, Musiani M. 2001. Distribution and viral load of type specific HPVs in different cervical lesions as detected by PCRELISA. J Clin Pathol 54:377-380. (Pubitemid 32458136)
23. zur Hausen H. 2002. Papillomaviruses and cancer: From basic studies to clinical application. Nat Rev Cancer 2:342-350.