A novel flow cytometric assay to quantify interactions between proteins and membrane lipids

Journal of Lipid Research

Volumn 50, Issue 6, 2009, Pages 1245-1254

  Temmerman, Koen ^a   Nickel, Walter ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

Competitive binding; Fibroblast growth factor 2; Fluorescence activated cell sorter; High throughput; Lipid binding; Liposome; Method; Phosphoinositide; Pleckstrin homology domain; Quantitative

Indexed keywords

CHOLESTEROL; FIBROBLAST GROWTH FACTOR; LIPOSOME; MEMBRANE LIPID; PHOSPHATIDYLCHOLINE; PHOSPHATIDYLINOSITOL; PHOSPHATIDYLINOSITOL 4,5 BISPHOSPHATE; PHOSPHATIDYLSERINE; PHOSPHOLIPASE C DELTA1; PLECKSTRIN HOMOLOGY DOMAIN OF PHOSPHOLIPASE C DELTA1; SPHINGOMYELIN; SULFORHODAMINE B; UNCLASSIFIED DRUG;

ARTICLE; BINDING KINETICS; FLOW CYTOMETRY; LIGHT SCATTERING; LIPID ANALYSIS; LIPID BILAYER; LIPID COMPOSITION; MEMBRANE CURVATURE; MEMBRANE STRUCTURE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN LIPID INTERACTION; QUANTITATIVE ASSAY; STOICHIOMETRY;

FIBROBLAST GROWTH FACTOR 2; FLOW CYTOMETRY; FLUORESCENT DYES; GREEN FLUORESCENT PROTEINS; LIGHT; LIPID BILAYERS; LIPOSOMES; MEMBRANE LIPIDS; PARTICLE SIZE; PHOSPHOLIPASE C DELTA; PROTEIN BINDING; PROTEINS; RECOMBINANT FUSION PROTEINS; SCATTERING, RADIATION; SENSITIVITY AND SPECIFICITY;

EID: 67649844238     PISSN: 00222275     EISSN: 15397262     Source Type: Journal    
DOI: 10.1194/jlr.D800043-JLR200     Document Type: Article

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