Evaluation of four methods of DNA recovery from Mycobacterium avium subspecies paratuberculosis present in intestine tissue of goats and comparative sensitivity of IS900 PCR with respect to culture for diagnosis of Johne's disease

Indian Journal of Experimental Biology

Volumn 46, Issue 8, 2008, Pages 579-582

  Singh, P K ^a   Singh, S V ^a   Singh, A V ^a   Sohal, J S ^a  

^a CENTRAL INSTITUTE FOR RESEARCH ON GOATS   (India)

Author keywords

DNA; Goat; Intestine tissues; IS900 PCR; Johne's disease; Mycobacterium avium paratuberculosis

Indexed keywords

ANIMALIA; CAPRA HIRCUS; MYCOBACTERIUM AVIUM; MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS;

BACTERIAL DNA;

ANIMAL; ARTICLE; BACTERIAL COUNT; COMPARATIVE STUDY; EVALUATION; GENETICS; GOAT; GROWTH, DEVELOPMENT AND AGING; INTESTINE; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; MYCOBACTERIUM PARATUBERCULOSIS; PARATUBERCULOSIS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

ANIMALS; COLONY COUNT, MICROBIAL; DNA, BACTERIAL; GOATS; INTESTINES; MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS; PARATUBERCULOSIS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 67649815188     PISSN: 00195189     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (12)

1. Pislak M, Ocepek M, Zabavnik Piano J & PogaNik M, Comparison of four methods for isolation of Mycobacterium avium subsp. paratuberculosis DNA from tissue samples. Slov Vet Res, 40 (2003) 91.
2. Singh N, Singh S V, Gupta V K, Sharma V D, Sharma R K & Katoch V M, Isolation and identification of Mycobacterium paratuberculosis from naturally infected goatherds in India. Indian J Vet Path, 20 (1996) 104.
3. Van Soolingen D, Hermans P W, de Haas P E, Soll D R & van Embden J D, Occurrence and stability of insertion sequences in Mycobacterium tuberculosis complex strains: evaluation of an insertion sequence-dependent DNA polymorphism as a tool in the epidemiology of tuberculosis. J Clin Microbiol, 29 (1991) 2578.
4. Fekete A, Bantle J A & Halling S M, Detection of Brucella by polymerase chain reaction in bovine fectal and maternal tissues. J Vet Diag Invest, 4 (1992) 79.
5. Vary P H, Andersen P R, Green E, Hermon-Taylor J & McFadden J J, Use of highly specific DNA probes and the polymerase chain reaction to detect Mycobacterium paratuberculosis in Johne's disease. J Clin Microbiol, 28 (1990) 933.
6. Choy E, Whittington R J, Marsh I, Marshall J & Campbell M T, A method for purification and characterization of Mycobacterium avium subsp. paratuberculosis from the intestinal mucosa of sheep with Johne's disease. Vet Microbiol, 64 (1998) 51.
7. Dimareli-Malli Z, Sarris K, Papadopoulos O, Iliadis N, Xenos G & Papadopoulos G, Caprine and ovine isolates of Mycobacterium paratuberculosis. Paratubercul News, 9 (1997)18.
8. Chiodini R J, Van Kruiningen, H J, Thayer W R & Coutu J A, Spheroplastic phase of mycobacteria isolated from patients with Crohn's disease. J Clin Microbiol, 24 (1986) 357.
9. Miller J M, Aljeny J B & Payer R, PCR detection of Mycobacterium tuberculosis complex and Mycobacterium avium organisms in formalin fixed tissue from culture negative ruminants. Vet Microbiol, 87 (2002) 15.
10. Kumar P, Singh S V, Bhatiya A K, Sevilla I, Singh A V, Whittington, R J, Juste R A, Gupta V K, Singh P K, Sohal J S & Vihan V S, Juvenile Capri-Paratuberculosis (JCP) in India; Incidence and characterization of by six diagnostic tests. Small Rumin Res, 73 (2007) 45.
11. Sivakumar P, Tripathi B N & Singh N, Detection of Mycobacterium avium subsp. paratuberculosis in intestinal and lymph node tissues of water buffaloes (Bubalus bubalis) by PCR and bacterial culture. Vet Microbiol, 108 (2005) 263.
12. Altwegg M, General problems associated with diagnostic applications of amplification methods. J Microbiol Methods, 23 (1995) 21.