Serine racemase with catalytically active lysinoalanyl residue

Journal of Biochemistry

Volumn 145, Issue 4, 2009, Pages 421-424

  Yamauchi, Takae ^a   Goto, Masaru ^b,c   Wu, Hui Yuan ^a   Uo, Takuma ^a,d   Yoshimura, Tohru ^a,e   Mihara, Hisaaki ^a   Kurihara, Tatsuo ^a   Miyahara, Ikuko ^b   Hirotsu, Ken ^b   Esaki, Nobuyoshi ^a  

^a KYOTO UNIVERSITY   (Japan)

^b Osaka Prefecture University   (Japan)

^c TOHO UNIVERSITY   (Japan)

^d UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

^e NAGOYA UNIVERSITY   (Japan)

Author keywords

D serine; Modification; Pyridoxal 5 phosphate; Racemase; Schizosaccharomyces pombe

Indexed keywords

BRAIN ENZYME; DEXTRO SERINE; N METHYL DEXTRO ASPARTIC ACID RECEPTOR; PYRIDOXAL 5 PHOSPHATE; RACEMASE; SCHIFF BASE; SERINE; ALANINE RACEMASE; ISOMERASE; LYSINOALANINE; SERINE RACEMASE;

ARTICLE; BRAIN; CATALYSIS; COENZYME; MASS SPECTROMETRY; NONHUMAN; SCHIZOSACCHAROMYCES POMBE; X RAY ANALYSIS; BIOCATALYSIS; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYMOLOGY; METABOLISM; SCHIZOSACCHAROMYCES; STATIC ELECTRICITY;

MAMMALIA; SCHIZOSACCHAROMYCES POMBE;

ALANINE RACEMASE; BIOCATALYSIS; CATALYTIC DOMAIN; ENZYME ACTIVATION; LYSINOALANINE; RACEMASES AND EPIMERASES; SCHIZOSACCHAROMYCES; SERINE; STATIC ELECTRICITY;

EID: 67649670256     PISSN: 0021924X     EISSN: 17562651     Source Type: Journal    
DOI: 10.1093/jb/mvp010     Document Type: Article

References (14)

1. Mothet, J.P., Parent, A.T., Wolosker, H., Brady, R.O., Linden, D.J., Ferris, C.D., Rogawski, M.A., and Snyder, S.H. (2000) D-Serine is an endogenous ligand for the glycine site of the N-methyl-D-aspartate receptor. Proc. Natl Acad. Sci. USA 97, 4926-4931
2. Wolosker, H. (2006) D-Serine regulation of NMDA receptor activity. Sci. STKE 356, 41
3. Sawa, A. and Snyder, S. H. (2002) Schizophrenia: diverse approaches to a complex disease. Science 296, 692-695
4. de Miranda, J., Panizzutti, R., Foltyn, V.N., and Wolosker, H. (2002) Cofactors of serine racemase that physiologically stimulate the synthesis of the N-methyl-D-aspartate (NMDA) receptor coagonist D-seine. Proc. Natl Acad. Sci. USA 99, 14542-14547
5. Wolosker, H., Sheth, K.N., Takahashi, M., Mothet, J.-P., Brady, R.O. Jr., Ferris, C.D., and Snyder, S.H. (1999) Purification of serine racemase: Biosynthesis of the neuromodulator D-serine. Proc. Natl Acad. Sci. USA. 96, 721-725
6. Wolosker, H.A., Blackshaw, S., and Snyder, S.H. (1999) Serine racemase: A glial enzyme synthesizing D-serine to regulate glutamate-N-methyl-D-aspartate neurotransmission. Proc. Natl Acad. Sci. USA. 96, 13409-13414
7. Mozzarelli, A. and Bettati, S. (2006) Exploring the pyridoxal 5′-phosphate-dependent enzyme. Chem. Rec. 6, 275-287
8. Badet, B., Roise, D., and Walsh, C.T. (1984) Inactivation of the dadB Salmonella typhimurium alanine racemase by D and L isomers of β-substituted alanines: kinetics, stoichiometry, active site peptide sequencing, and reaction mechanism. Biochemistry 23, 5188-5194
9. Esaki, N. and Walsh, C. T. (1986) Biosynthetic alanine racemase of Salmonella typhimurium: purification and characterization of the enzyme encoded by the alr gene. Biochemistry 25, 3261-3267
10. Roise, D., Soda, K., Yagi, T., and Walsh, C.T. (1984) Inactivation of the Pseudomonas striata broad specificity amino acid racemase by D and L isomers of β-substituted alanines: kinetics, stoichiometry, active site peptide, and mechanistic studies. Biochemistry 23, 5195-5201
11. Burkhard, P., Tai, C.H., Ristroph, C.M., Cook, P.F., and Jansonius, J.N. (1999) Ligand binding induces a large conformational change in O-acetylserine sulfhydrylase from Salmonella typhimurium. J. Mol. Biol. 291, 941-953
12. Omi, R., Goto, M., Miyahara, I., Mizuguchi, H., Hayashi, H., Kagamiyama, H., and Hirotsu, K. (2003) Crysral structures of threonine synthase from Thermus thermophilus HB8: conformational change, substrate recognition, and mechanism. J. Biol. Chem. 278, 46035-46045
13. Watanabe, A., Yoshimura, T., Mikami, B., Hayashi, H., Kagamiyama, H., and Esaki, N. (2002) Reaction mechanism of alanine racemase from Bacillus stearothermophilus: X-ray crystallographic studies of the enzyme bound with N-(5′- phosphopyridoxyl)alanine. J. Biol. Chem. 277, 19166-19172
14. Panizzutti, R., De Miranda, J., Ribeiro, C., Engelender, S., and Wolosker, H. (2001) A new strategy to decreace N-methyl-D-aspartate (NMDA) receptor coactivation: inhibition of D-serine synthesis by converting serine racemase into an eliminase. Proc. Natl Acad. Sci. USA 98, 5294-5299