PCR-based detection of enterotoxigenic isolates of Bacillus cereus from tropical seafood

Indian Journal of Medical Research

Volumn 129, Issue 3, 2009, Pages 316-320

  Das, Sanjoy ^a   Surendran, P K ^a,b   Thampuran, Nirmala ^a  

^a CENTRAL INSTITUTE OF FISHERIES TECHNOLOGY   (India)

^b NONE   (India)

Author keywords

Bacillus cereus; Enterotoxin; PCR; RPLA; Virulent gene

Indexed keywords

ENTEROTOXIN; ENTEROTOXIN, BACILLUS CEREUS;

AGAR MEDIUM; ARTICLE; BACILLUS CEREUS; BACILLUS CEREUS INFECTION; BACTERIUM CULTURE; BACTERIUM ISOLATION; CLAM; DIARRHEA; FISH; FISH DISEASE; LATEX AGGLUTINATION TEST; NONHUMAN; PLATING MEDIUM; POLYMERASE CHAIN REACTION; POLYMIXIN PYRUVATE EGG YOLK MANNITOL BROMOCRESOL PURPLE AGAR; SEA FOOD; SHRIMP; ANIMAL; BIVALVE; FOOD CONTROL; FOOD POISONING; GENETICS; INDIA; METHODOLOGY; MICROBIOLOGY; PATHOGENICITY; PENAEIDAE; VIRULENCE;

ANIMALS; BACILLUS CEREUS; BIVALVIA; ENTEROTOXINS; FISHES; FOOD MICROBIOLOGY; FOOD POISONING; INDIA; PENAEIDAE; POLYMERASE CHAIN REACTION; SEAFOOD; VIRULENCE;

EID: 67649660060     PISSN: 09715916     EISSN: 09715916     Source Type: Journal    
DOI: None     Document Type: Article

References (20)

1. Schoeni JL, Wong AC. Bacillus cereus food poisoning and its toxins. J Food Prot 2005; 68: 636-48.
2. Dierick K, Van Coillie E, Swiecicka I, Meyfroidt G, Devlieger H, Meulemans A, et al. Fatal family outbreak of Bacillus cereus - associated food poisoning. J Clin Microbiol 2005; 43: 4277-9.
3. Evreux F, Delaporte B, Leret N, Buffet-Janvresse C, Morel A. A case of fatal neonatal Bacillus cereus meningitis. Arch Pediatr 2007; 14: 365-8.
4. Meer RR, Baker J, Bodyfelt FW, Griffiths MW. Psychotropic Bacillus spp. in fluid milk products: a review. J Food Prot 1991; 54: 969-79.
5. Te Giffel MC, Beumer RR, Granum PE, Rombouts FM. Isolation and characterisation of Bacillus cereus from pasteurised milk in household refrigerators in the Netherlands. Int J Food Microbiol 1997; 34: 307-18.
6. Valero M, Hernández-Herrero LA, Giner MJ. Survival, isolation and characterization of a psychrotrophic Bacillus cereus strain from a mayonnaise-based ready-to-eat vegetable salad. Food Microbiol 2007; 24: 671-7.
7. Novak JS, Call J, Tomasula P, Luchansky JB. An assessment of pasteurization treatment of water, media, and milk with respect to Bacillus spores. J Food Prot 2005; 68: 751-7.
8. Kamat AS, Nerkar DP, Nair PM. Bacillus cereus in some indian foods, incidence and antibiotic, heat and radiation resistance. J Food Safety 1989; 10: 31-41.
9. Heinrichs JH, Beecher DJ, Macmillan JD, Zilinskas BA. Molecular cloning and characterization of the hbla gene encoding the B component of hemolysin BL from Bacillus cereus. J Bacteriol 1993; 175: 6760-6.
10. Agata N, Ohta M, Arakawa Y, Mori M. The bceT gene of Bacillus cereus encodes an enterotoxic protein. Microbiology 1995; 141: 983-8.
11. Asano SI, Nukumizu Y, Bando H, Iizuka T, Yamamoto T. Cloning of novel enterotoxin genes from Bacillus cereus and Bacillus thuringiensis. Appl Environ Microbiol 1997; 63: 1054-7.
12. Granum PE, Brynestad S, Kramer JM. Analysis of enterotoxin production by Bacillus cereus from dairy products, food poisoning incidents and non-gastrointestinal infections. Int J Food Microbiol 1993; 17: 269-79.
13. Szabo RA, Todd ECD, Rayman M. Twenty-four hours isolation and confirmation of Bacillus cereus in foods. J Food Prot 1984; 47: 856-60.
14. Rhodehamel EJ, Harmon SM. Bacillus cereus. Bacteriological Analytical Manual online 8th ed. Revision 1998. Chapter 14 [monograph on the internet]. U.S. Food and Drug Administration; 2001. Available from http://www.cfsan.fda.gov/~ebam/bam-14.html#authors, accessed on December 12, 2007.
15. Collins, CH, Lyne, PM, Grange JM. Collins and Lyne's microbiological methods. 7th ed. London: Arnold; 2001.
16. Phelps RJ, McKillip JL. Enterotoxin production in natural isolates of Bacillaceae outside the Bacillus cereus group. Appl Environ Microbiol 2002; 68: 3147-51.
17. Chang YH, Shangkuan YH, Lin HC, Liu HW. PCR assay of the groEL gene for detection and differentiation of Bacillus cereus group cells. Appl Environ Microbiol 2003; 69: 4502-10.
18. Mäntynen V, Lindstrom K. A rapid PCR-based DNA test for enterotoxic Bacillus cereus. Appl Environ Microbiol 1998; 64: 1634-9.
19. Agarwal RK, Kapoor KN, Bachhil VN, Singh BR, Kumar A, Bhilegaonkar KN. Studies on toxigenicity of Bacillus cereus strains isolated from food. Indian J Comp Microbiol Immunol Infect Dis 1997; 18: 162-5.
20. Relman DA, Persing DH. Genotypic methods for microbial identification. In: Persing DH, editor. PCR protocols for emerging infectious diseases. Washington DC: American Society for Microbiology, ASM Press; 1996. p. 3-31.