Detection of the mitochondrial and catalytically active alanine aminotransferase in human tissues and plasma

International Journal of Molecular Medicine

Volumn 23, Issue 5, 2009, Pages 621-631

  Glinghammar, Björn ^a   Rafter, Ingalill ^a   Lindström, Anna Karin ^b   Hedberg, Jesper J ^a   Andersson, Håkan B ^b   Lindblom, Per ^a   Berg, Anna Lena ^b   Cotgreave, Ian ^a  

^a ASTRAZENECA R AND D   (Sweden)

^b ASTRAZENECA R AND D   (Sweden)

Author keywords

ALAT; ALT; Biomarker; GPT2; Hepatotoxicity; Liver; Plasma; Serum; Tissue expression

Indexed keywords

ALANINE AMINOTRANSFERASE; ALANINE AMINOTRANSFERASE 1; ALANINE AMINOTRANSFERASE 2; LIVER EXTRACT; UNCLASSIFIED DRUG;

ALANINE AMINOTRANSFERASE BLOOD LEVEL; ARTICLE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOSOL; ENDOPLASMIC RETICULUM; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME KINETICS; ENZYME LOCALIZATION; ENZYME STABILITY; HEART MUSCLE CELL; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; IMMUNOPRECIPITATION; LIVER CELL; MITOCHONDRION; MUSCLE CELL; PANCREAS ISLET; PRIORITY JOURNAL; PROTEIN EXPRESSION; SKELETAL MUSCLE; WESTERN BLOTTING;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; ALANINE TRANSAMINASE; CELLS, CULTURED; FEMALE; HEPATOCYTES; HUMANS; LIVER; MALE; MIDDLE AGED; MITOCHONDRIA; MUSCLE, SKELETAL; PLASMA; RECOMBINANT PROTEINS; SERUM; SUBSTRATE SPECIFICITY; YOUNG ADULT;

EID: 67649424237     PISSN: 11073756     EISSN: 1791244X     Source Type: Journal    
DOI: 10.3892/ijmm_00000173     Document Type: Article

References (34)

1. De Rosa G, Burk TL and Swick RW: Isolation and characterization of mitochondrial alanine aminotransferase from porcine tissue. Biochim Biophys Acta 567: 116-124, 1979.
2. Swick RWBPLSJL: The metabolism of mitochondrial proteins. I. Distribution and charachterization of the isozymes of alanine aminotrnasferase in rat liver. J Biol Chem 240: 3334-3340, 1965.
3. Takeda YIATHIH: The biochemistry of animal cells. The effect of corticoteroids on leakage of enzymes from dispersed rat liver cells. J Biol Chem 239: 3590-3596, 1964.
4. Ishiguro M, Takio K, Suzuki M, Oyama R, Matsuzawa T and Titani K: Complete amino acid sequence of human liver cytosolic alanine aminotransferase (GPT) determined by a combination of conventional and mass spectral methods. Biochemistry 30: 10451-7, 1991.
5. Yang RZ, Blaileanu G, Hansen BC, Shuldiner AR and Gong DW: cDNA cloning, genomic structure, chromosomal mapping, and functional expression of a novel human alanine aminotransferase. Genomics 79: 445-450, 2002.
6. Lindblom P, Rafter I, Copley C, Andersson U, Hedberg JJ, Berg AL, Samuelsson A, Hellmold H, Cotgreave I and Glinghammar B: Isoforms of alanine aminotransferases in human tissues and serum - differential tissue expression using novel antibodies. Arch Biochem Biophys 466: 66-77, 2007.
7. Sohocki MM, Sullivan LS, Harrison WR, Sodergren EJ, Elder FF, Weinstock G, Tanase S and Daiger SP: Human glutamate pyruvate transaminase (GPT): localization to 8q24.3, cDNA and genomic sequences, and polymorphic sites. Genomics 40: 247-252, 1997.
8. Karmen AWFLJS: Transaminase activity in human blood. J Clin Invest 34: 126-131, 1955.
9. Draper RP, Waterfield CJ, York MJ and Timbrell JA: Studies on the muscle toxicant 2,3,5,6-tetramethyl p-phenylenediamine: effects on various biomarkers including urinary creatine and taurine. Arch Toxicol 69: 111-117, 1994.
10. O'Brien PJ, Slaughter MR, Polley SR and Kramer K: Advantages of glutamate dehydrogenase as a blood biomarker of acute hepatic injury in rats. Lab Anim 36: 313-321, 2002.
11. Valentine BA, Blue JT, Shelley SM and Cooper BJ: Increased serum alanine aminotransferase activity associated with muscle necrosis in the dog. J Vet Intern Med 4: 140-143, 1990.
12. Waner T and Nyska A: The toxicological significance of decreased activities of blood alanine and aspartate aminotransferase. Vet Res Commun 15: 73-78, 1991.
13. Dhami MS, Drangova R, Farkas R, Balazs T and Feuer G: Decreased aminotransferase activity of serum and various tissues in the rat after cefazolin treatment. Clin Chem 25: 1263-1266, 1979.
14. Yamada RH, Wakabayashi Y and Iwashima A: Inhibition of serum aspartate aminotransferase induced by isoniazid administration in mice. Acta Vitaminol Enzymol 6: 289-293, 1984.
15. Jadhao SB, Yang RZ, Lin Q, Hu H, Anania FA, Shuldiner AR and Gong DW: Murine alanine aminotransferase: cDNA cloning, functional expression, and differential gene regulation in mouse fatty liver. Hepatology 39: 1297-1302, 2004.
16. Lindena J, Sommerfeld U, Hopfel C and Trautschold I: Catalytic enzyme activity concentration in tissues of man, dog, rabbit, guinea pig, rat and mouse. Approach to a quantitative diagnostic enzymology, III. Communication. J Clin Chem Clin Biochem 24: 35-47, 1986.
17. Ladue JS and Wroblewski F: Serum glutamic pyruvic transaminase SGP-T in hepatic disease: a preliminary report. Ann Intern Med 45: 801-811, 1956.
18. Gubern G, Imperial S, Busquets M and Cortes A: Partial characterization of the alanine aminotransferase isoenzymes from human liver. Biochem Soc Trans 18: 1288-1289, 1990.
19. Williamson DH, Lopes-Vieira O and Walker B: Concentrations of free glucogenic amino acids in livers of rats subjected to various metabolic stresses. Biochem J 104: 497-502, 1967.
20. DeRosa G and Swick RW: Metabolic implications of the distribution of the alanine aminotransferase isoenzymes. J Biol Chem 250: 7961-7967, 1975.
21. Gubern G, Imperial S, Busquets M and Cortes A: Subcellular distribution of alanine aminotransferase activity in human liver. Biochem Soc Trans 18: 1287-1288, 1990.
22. Hird FJ and Rowsell EV: Additional transaminations by insoluble particle preparations of rat liver. Nature 166: 517-518, 1950.
23. Pette D and Luh W: Constant-proportion groups of multilocated enzymes. Biochem Biophys Res Commun 8: 283-287, 1962.
24. Meton I, Egea M, Fernandez F, Eraso MC and Baanante IV: The N-terminal sequence directs import of mitochondrial alanine aminotransferase into mitochondria. FEBS Lett 566: 251-254, 2004.
25. Swick RW, Barnstein PL and Stange JL: The metabolism of mitochondrial proteins. II. The response of the isozymes of alanine aminotransferase to diet and hormones. J Biol Chem 240: 3341-3345, 1965.
26. Fujiwara S, Sakuraba H and Noguchi T: Identification of chicken liver mitochondrial alanine: 2-oxoglutarate aminotransferase and its response to starvation. Arch Biochem Biophys 310: 497-503, 1994.
27. Davison TF and Langslow DR: Changes in plasma glucose and liver glycogen following the administration of gluconeogenic precursors to the starving fowl. Comp Biochem Physiol A 52: 645-649, 1975.
28. Brady LJ, Romsos DR, Brady PS, Bergen WG and Leveille GA: The effects of fasting on body composition, glucose turnover, enzymes and metabolites in the chicken. J Nutr 108: 648-657, 1978.
29. Bang NU and Ladue JS: Comparison of the serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase and lactic dehydrogenase activity following acute myocardial infarction. Dis Chest 41: 384-391, 1962.
30. Giesen PL, Peltenburg HG, de Zwaan C, Janson PC, Flendrig JG and Hermens WT: Greater than expected alanine aminotransferase activities in plasma and in hearts of patients with acute myocardial infarction. Clin Chem 35: 279-283, 1989.
31. Barshes NR, Lee TC, Goodpastor SE, Balkrishnan R, Schock AP, Mote A, Brunicardi FC, Alejandro R, Ricordi C and Goss JA: Transaminitis after pancreatic islet transplantation. J Am Coll Surg 200: 353-361, 2005.
32. Liu L, Zhong S, Yang R, Hu H, Yu D, Zhu D, Hua Z, Shuldiner AR, Goldstein R, Reagan WJ and Gong W: Expression, purification, and initial characterization of human alanine aminotrasferase (ALT) isoenzyme 1 and 2 in High-five insect cells. Protein Expr Purif 60: 225-231, 2008.
33. Mukorah F, Razunguzwa B and Masola B: Stabilization of rat liver mitochondrial alanine aminotransferase with ethanol and trehalose. Cryobiology 37: 300-308, 1998.
34. Wada H, Teranishi H, Kagamiyama H, Ohyanagi H, Shirakawa M, Mitsuno T, Fuse K and Sawada Y: A simple immunological method for differential determination of serum glutamicoxaloacetic transaminase isoenzymes. (I) Using anti-human-GOT antibody. Med J Osaka Univ 29: 181-190, 1978.