Spuriously high thyrotropin values due to anti-thyrotropin antibodies in adult patients

Clinical Chemistry and Laboratory Medicine

Volumn 47, Issue 5, 2009, Pages 604-606

  Verhoye, Eline ^a   Van Den Bruel, Annick ^a   Delanghe, Joris R ^b   Debruyne, Evi ^b   Langlois, Michel R ^a  

^a Department of Clinical Chemistry ^*  (Belgium)

^b GHENT UNIVERSITY HOSPITAL   (Belgium)

Author keywords

Antibody interference; Electrochemiluminescent immunoassay (ECLIA); Polyethylene glycol (PEG); Thyroid stimulating hormone (TSH)

Indexed keywords

IMMUNOGLOBULIN; MACROGOL; PROTEIN A; RHEUMATOID FACTOR; THYROTROPIN; THYROTROPIN RECEPTOR ANTIBODY;

ADSORPTION; ADULT; ARTICLE; BIOCHEMISTRY; CLINICAL ARTICLE; CONCENTRATION (PARAMETERS); EUTHYROIDISM; FEMALE; GEL FILTRATION CHROMATOGRAPHY; HUMAN; HYPERTHYROIDISM; IMMUNOASSAY; PRECIPITATION; PRIORITY JOURNAL; PROTEIN BLOOD LEVEL;

ADULT; FALSE POSITIVE REACTIONS; FEMALE; HUMANS; IMMUNOASSAY; IMMUNOGLOBULINS, THYROID-STIMULATING; MIDDLE AGED; POLYETHYLENE GLYCOLS; PRECIPITIN TESTS; RECEPTORS, THYROTROPIN; THYROID FUNCTION TESTS; THYROTROPIN; YOUNG ADULT;

EID: 67649223907     PISSN: 14346621     EISSN: 14374331     Source Type: Journal    
DOI: 10.1515/CCLM.2009.138     Document Type: Article

References (12)

1. Despres N, Grant AM. Antibody interference in thyroid assays: a potential for clinical misinformation. Clin Chem 1998;44:440-54.
2. Sapin R, Agin A, Gasser F. Misleading high thyrotropin results obtained with a two-site immunometric assay involving a chimeric antibody. Clin Chem 2004;50:946-8.
3. Marks V. False-positive immunoassay results: a multi-center survey or erroneous immunoassay results from assays of 74 analytes in 10 donors from 66 laboratories in seven countries. Clin Chem 2002;48:2008-16.
4. Sapin R, d'Herbomez M, Schlienger JL, Wemeau JL. Anti-thyrotropin antibody interference in thyrotropin assays. Clin Chem 1998;44:2557-9.
5. Ismail AA. A radical approach is needed to eliminate interference from endogenous antibodies in immuno-assays. Clin Chem 2005;51:25-6.
6. Tamaki H, Takeoka K, Nishi I, Shindoh Y, Tsukada Y, Amino N. Novel thyrotropin (TSH)-TSH antibody complex in a healthy woman and her neonates. Thyroid 1995;5:299-303.
7. Lazarus JH, John R, Ginsberg J, Hughes IA, Shewring G, Smith BR, et al. Transient neonatal hyperthyrotrophinae-mia: a serum abnormality due to transplacentally acquired antibody to thyroid stimulating hormone. Br Med J 1983;286:592-4.
8. Newman JD, Bergman PB, Doery JC, Balazs ND. Factitious increase in thyrotropin in a neonate caused by a maternally transmitted interfering substance. Clin Chem 2006;52:541-2.
9. Halsall DJ, Fahie-Wilson MN, Hall SK, Barker P, Anderson J, Gama R, et al. Macro thyrotropin-IgG complex causes factitious increases in thyroid-stimulating hormone screening tests in a neonate and mother. Clin Chem 2006;52:1968-9; author reply 1969-70.
10. Kavanagh L, McKenna TJ, Fahie-Wilson MN, Gibney J, Smith TP. Specificity and clinical utility of methods for the detection of macroprolactin. Clin Chem 2006;52: 1366-72.
11. Schiettecatte J, De Schepper J, Velkeniers B, Smitz J, Van Steirteghem A. Rapid detection of macroprolactin in the form of prolactin-immunoglobulin G complexes by immunoprecipitation with anti-human IgG-agarose. Clin Chem Lab Med 2001;39:1244-8.
12. Fahie-Wilson M, Halsall D. Polyethylene glycol precipitation: proceed with care. Ann Clin Biochem 2008;45: 233-5.