Expression of the chicken lysozyme gene in potato enhances resistance to infection by Erwinia carotovora subsp. atroseptica

American Journal of Potato Research

Volumn 77, Issue 3, 2000, Pages 191-199

  Serrano, Carolina ^a   Arce Johnson, Patricio ^a   Torres, Hebert ^b   Gebauer, Marlene ^a   Gutierrez, Mónica ^b   Moreno, Mauricio ^a   Jordana, Xavier ^a   Venegas, Alejandro ^a   Kalazich, Julio ^b   Holuigue, Loreto ^a  

^a PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE   (Chile)

^b INSTITUTO DE INVESTIGACIONES AGROPECUARIAS   (Chile)

Author keywords

Bacterial resistance; Blackleg; Erwinia carotovora; Plant transformation; Soft rot

Indexed keywords

BACTERIAL INFECTION; BLACKLEG DISEASE; CLONE; CULTIVAR; DISEASE RESISTANCE; GENE CHLY; GENE EXPRESSION; GENETIC SCREENING; GENETIC TRANSFORMATION; LYSOZYME; POTATO; SOFT ROT; TRANSGENE; TRANSGENIC PLANT;

AGROBACTERIUM; BACTERIA (MICROORGANISMS); EMBRYOPHYTA; ERWINIA; ERWINIA CAROTOVORA; GALLUS GALLUS; PECTOBACTERIUM ATROSEPTICUM; PECTOBACTERIUM CAROTOVORUM; RHIZOBIUM; SOLANUM TUBEROSUM;

EID: 6744266669     PISSN: 1099209X     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02853944     Document Type: Article

References (42)

1. Allefs, S.J.H.M., E.R. De Jong, D.E.A Florack, C. Hoogendoorn, and W.J. Stiekema. 1996. Erwinia soft rot resistance of potato cultivars expressing antimicrobial peptide tachyplesin I. Mol Breed 2:97-105.
2. Amasino, R.M. 1986. Acceleration of nucleic acid hybridization rate by polyethylene glycol. Anal Biochem 152:304-307.
3. Arce, P., M. Moreno, M. Gutierrez, M. Gebauer, P. Dell'Orto, H. Torres, I. Acuña, P. Oliger, A. Venegas, X. Jordana, J. Kalazich, and L. Holuigue. 1999. Enhanced resistance to bacterial infection by Erwinia carotovora subsp. atroseptica in transgenic potato plants expressing the attacin or the cecropin SB-37 genes. Am Potato J 75:1-9.
4. Austin, S., E. Lojkowska, M.K. Ehlenfeldt, A. Kelman, and J. Helgeson. 1988. Fertile interspecific somatic hybrids of Solanum: a novel source of resistance to Erwinia soft rot. Phytopathology 78:1216-1220.
5. Ausubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, and K. Struhl. 1997. Current protocols in molecular biology. New York: John Wiley & Sons, Inc.
6. Bevan, M., W. Barnes, and M-D. Chilton. 1983. Structure and transcription of nopaline synthase gene region of T-DNA. Nucl Acids Res 11:369-385.
7. Boman, H.G. 1995. Peptide antibiotics and their role in innate immunity. Annu Rev Immunol 13:61-92.
8. Bourne, W.F., D.C. McCalmont, and R.L. Wastie. 1981. Assessing potato tubers for susceptibility to bacterial soft rot (Erwinia carotovora subsp. atroseptica). Potato Res 24:409-415.
9. Brain, R.A. and M.C.M. Pérombelon. 1988. Methods of testing potato cultivars for resistance to soft rot of tubers caused by Erwinia carotovora subsp. atroseptica. Plant Pathol 37:431-437.
10. Broekaert, W.F., F.R.G. Terras, B.P.A. Cammue, and R.W. Osborn. 1995. Plant defensins: novel antimicrobial peptides as components of the host defense system. Plant Physiol 108:1353-1358.
11. Cao, H., X. Li, and X. Dong. 1998. Generation of broad-spectrum disease resistance by overexpression of an essential regulatory gene in systemic acquired resistance. Proc Natl Acad Sci USA 95:6531-6536.
12. Carmona, M.J., A. Molina, J.A. Fernández, J.J. López-Fando, and F. García-Olmedo. 1993. Expression of the α-thionin gene from barley in tobacco confers enhanced resistance to bacterial pathogens. Plant J 3:457-462.
13. Ciampi-Panno, L. and N. Andrade-Soto. 1984. Preliminary evaluation of bacterial soft rot resistance in native Chilean potato clones. Am Potato J 61:109-112.
14. Destéfano-Beltran, L., P. Nagpala, M.S. Cetiner, J.H. Dodds, and J.M. Jaynes. 1990. Enhancing bacterial and fungal disease resistance in plants: application to potato. In: Vayda, M.E. and W.D. Park (eds.), The molecular and cellular biology of the potato, Oxon, UK C.A.B. International Publishers, pp 205-221.
15. During, K. 1996. Genetic engineering for resistance to bacteria in transgenic plants by introduction of foreign genes. Mol Breed 2:297-305.
16. Düring, K., P. Porsch, M. Fladung, and H. Lörz. 1993. Transgenic potato plants resistant to the phytopathogenic bacterium Erwinia carotovora. Plant J 3:587-598.
17. Fang, G., S. Hammar, and R. Grumet. 1992. A quick and inexpensive method for removing polysaccharides from plant genomic DNA. BioTechniques 13:52-54.
18. García-Olmedo, F., A. Molina, A. Segura, and M. Moreno. 1995. The defensive role of nonspecific lipid-transfer proteins in plants. Trends Microbiol 3:72-74.
19. Hultmark, D. 1993. Immune reactions in Drosophila and other insects: A model for innate immunity. Trends Genet 9:178-183.
20. Jach, G., B. Görnhardt, J. Mundy, J. Logemann, E. Pinsdorf, R. Leah, J. Schell, and C. Maas. 1995. Enhanced quantitative resistance against fungal disease by combinatorial expression of different barley antifungal proteins in transgenic tobacco. Plant J 8:97-109.
21. Jaynes, J.M., P. Nagpala, L. Destéfano-Beltrán, J.H. Huang, J.H. Kim, T. Denny, and S. Cetiner. 1993. Expression of a Cecropin B lytic peptide analog in transgenic tobacco confers enhanced resistance to bacterial wilt caused by Pseudomonas solanacearum. Plant Sci 89:43-53.
22. Jollès, P. and J. Jollès. 1984. What's new in lysozyme research? Mol Cell Biochem 63:165-189.
23. Jung, A., A.E. Sippel, M. Grez, and G. Schütz. 1980. Exons encode functional and structural units of chicken lysozyme. Proc Natl Acad Sci USA 77:5759-5763.
24. Kay, R., A. Chan, M. Daaly, and J. McPherson. 1987. Duplication of CaMV 35S promoter sequences creates a strong enhancer for plant genes. Science 236:1299-1302.
25. Lapwood, D.H., P.J. Read, and J. Spokes. 1984. Methods for assessing the susceptibility of potato tubers of different cultivars to rotting by Erwinia carotovora subsp. atroseptica and carotovora. Plant Pathol 33:13-20.
26. Logemann, J., J. Schell, and L. Willmitzer. 1987. Improved method for the isolation of RNA from plant tissues. Anal Biochem 163:16-20.
27. Molina, A., P. Ahl-Goy, A. Fraile, R. Sanchez-Monge, and F. García-Olmedo. 1993. Inhibition of bacterial and fungal plant pathogens by thionins of types I and II. Plant Sci 92:169-177.
28. Molina, A. and F. García-Olmedo. 1997. Enhanced tolerance to bacterial pathogens caused by the transgenic expression of barley lipid transfer protein LTP2. Plant J 12:669-675.
29. Mourgues, F., M-N. Brisset, and E. Chevreau. 1998. Strategies to improve resistance to bacterial diseases through genetic engineering. Trends Biotechnol 16:203-210.
30. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-479.
31. Nakajima, H., T. Muranaka, F. Ishige, K. Akutsu, and K Oeda. 1997. Fungal and bacterial disease resistance in transgenic plants expressing human lysozyme. Plant Cell Rep 16:674-679.
32. Panapoulos, N.J., E. Hatziloukas, and A.S. Afendra. 1996. Transgenic crops resistance to bacteria Field Crop Res 45:85-97.
33. Rabot, B., C. Pasco, and J. Schmidt. 1994. Assessing six Austrian potato cultivars for resistance to Erwinia carotovora subsp. atroseptica. Potato Res 37:197-203.
34. Rousselle-Bourgeois, F. and S. Priou. 1995. Screening tuber-bearing Solanum spp. for resistance to soft rot caused by Erwinia carotovora ssp. atroseptica. Potato Res 38:111-118.
35. Salmond, G.P.C. 1994. Secretion of extracellular virulence factors by plant pathogenic bacteria. Annu Rev Phytopath 32:181-200.
36. Shah, D.M. 1997. Genetic engineering for fungal and bacterial diseases. Curr. Opin. Biotechnol 8:208-214.
37. Singh, A., T-H. Kao, and J-J. Lin. 1993. Transformation of Agrobacterium tumefaciens with T-DNA vectors using high-voltage electroporation. FOCUS 15:84-87.
38. Thilmony, R.L., Z. Chen, R.A. Bressan, and G.B. Martin. 1995. Expression of the tomato Pto gene in tobacco enhances resistance to Pseudomonas syringae pv tabaci expressing avrPto. Plant Cell 7:1529-1536.
39. Trudel, J., C. Potvin, and A. Asselin. 1992. Expression of active hen egg white lysozyme in transgenic tobacco. Plant Sci 87:55-67.
40. Trudel, J., C. Potvin, and A. Asselin. 1995. Secreted hen lysozyme in transgenic tobacco: recovery of bound enzyme and in vitro growth inhibition of plant pathogens. Plant Sci 106:55-62.
41. van Soest, L.J.M. 1983. Evaluation and distribution of important properties in the German-Netherlands potato collection. Potato Res 26:109-121.
42. 2-generating glucose oxidase in transgenic potato plants. Plant Cell 7:1357-1368.