In vitro and in vivo quantification of elicitin expression in Phytophthora cinnamomi

Physiological and Molecular Plant Pathology

Volumn 73, Issue 1-3, 2008, Pages 48-57

  Horta, Marília ^a,b   Sousa, Nelson ^a   Coelho, Ana C ^a   Neves, Dina ^a   Cravador, Alfredo ^a,b  

^a UNIVERSITY OF ALGARVE   (Portugal)

^b UNIVERSITY OF TRÁS OS MONTES AND ALTO DOURO   (Portugal)

Author keywords

Elicitin expression; Gene silencing; Pathogenesis; Phytophthora cinnamomi; Quercus; Real time RT PCR; Transformation

Indexed keywords

PHYTOPHTHORA CINNAMOMI; QUERCUS; QUERCUS SUBER;

EID: 67349285448     PISSN: 08855765     EISSN: 10961178     Source Type: Journal    
DOI: 10.1016/j.pmpp.2009.02.003     Document Type: Article

References (52)

1. Panabiéres F., Marais A., Le Berre J.-Y., Penot I., Fournier D., and Ricci P. Characterization of a gene cluster of Phytophthora cryptogea which codes for elicitins, proteins inducing a hypersensitive-like response in tobacco. Molecular Plant-Microbe Interactions 8 (1995) 996-1003
2. Duclos J., Fauconnier A., Coelho A.C., Bollen A., Cravador A., and Godfroid E. Identification of an elicitin gene cluster in Phytophthora cinnamomi. DNA Sequence. Journal of Sequencing and Mapping 9 (1998) 231-237
3. Jiang R.H.Y., Dawe A.L., Weide R., van Staveren M., Peters S., Nuss D., et al. Elicitin genes in Phytophthora infestans are clustered and interspersed with various transposon-like elements. Molecular Genetics and Genomics 273 (2005) 20-32
4. Jiang R.H.Y., Tyler B.M., Whisson S.C., Hardham A.R., and Govers F. Ancient origin of elicitin gene clusters in Phytophthora genomes. Molecular Biology and Evolution 23 (2006) 338-351
5. Pernollet J.C., Sallatin M., Sallé-Tourne M., and Huet J.C. Elicitin isoforms from seven Phytophthora species: comparison of their physico-chemical properties and toxicity to tobacco and other plant species. Physiological and Molecular Plant Pathology 42 (1993) 53-67
6. Duclos J., Trincão Aurélio M., Graça J., Coelho A.C., Fauconnier A., Jacquet A., et al. Identification of an elicitin gene cluster in Phytophthora cinnamomi and analysis of the necrotic activity of a purified recombinant β-cinnamomin. Proceedings of the twelfth forum for applied biotechnology (1998), University of Gent, Belgium 1695-1698
7. Ponchet M., Panabières F., Milat M.-L., Mikes V., Montillet J.-L., Suty L., et al. Are elicitins cryptograms in the plant - oomycete communications?. Cellular and Molecular Life Sciences 56 (1999) 1020-1047
8. Baillieul F., Fritig B., and Kauffmann S. Occurrence among Phytophthora species of a glycoprotein eliciting a hypersensitive response in tobacco and its relationships with elicitins. Molecular Plant-Microbe Interactions 9 (1996) 214-216
9. Kamoun S., Lindqvist H., and Govers F. A novel class of elicitin-like genes from Phytophthora infestans. Molecular Plant-Microbe Interactions 10 (1997) 1028-1030
10. Panabières F., Amselemb J., Galiana E., and Berre J. Gene identification in the oomycete pathogen Phytophthora parasitica during in vitro vegetative growth through expressed sequence tags. Fungal Genetics and Biology 42 (2005) 611-623
11. Qutob D., Huitema E., Gijzen M., and Kamoun S. Variation in structure and activity among elicitins from Phytophthora sojae. Molecular Plant Pathology 4 (2003) 119-124
12. Boissy G., O'Donohue M., Gaudemer O., Perez V., Pernollet J.C., and Brunie S. The 2.1 Ä structure of an elicitin-ergosterol complex: a recent addition to the sterol carrier protein family. Protein Science 8 (1999) 1191-1199
13. Mikes V., Milat M.L., Ponchet M., Ricci P., and Blein J.P. The fungal elicitor cryptogein is a sterol carrier protein. FEBS Letters 416 (1997) 190-192
14. Mikes V., Milat M.L., Ponchet M., Panabières F., Ricci P., and Blein J.-P. Elicitins, proteinaceous elicitors of plant defense, are a new class of sterol carrier proteins. Biochemical and Biophysical Research Communications 245 (1998) 133-139
15. Osman H., Mikes V., Milat M.L., Ponchet M., Marion D., Prange T., et al. Fatty acids bind to the fungal elicitor cryptogein and compete with sterols. FEBS Letters 489 (2001) 55-58
16. Rodrigues M.L., Archer M., Martel P., Miranda S., Thomaz M., Enguita F.J., et al. Crystal structures of the free and sterol-bound forms of β-cinnamomin. Biochimica et Biophysica Acta 1764 (2006) 110-121
17. Vauthrin S., Mikes V., Milat M.L., Ponchet M., Maume B., Osman H., et al. Elicitins trap and transfer sterols from micelles, lipossomes and plant plasma membranes. Biochimica et Biophysica Acta 1419 (1999) 335-342
18. Grant B.R., Ebert D., and Gayler K.R. Elicitins: proteins in search of a role?. Australasian Plant Pathology 25 (1996) 148-157
19. Kamoun S., van West P., de Jong A.J., de Groot K.E., Vleeshouwers V.G.A.A., and Govers F. A gene encoding a protein elicitor of Phytophthora infestans is down-regulated during infection of potato. Molecular Plant-Microbe Interactions 10 (1997) 13-20
20. Nespoulos C., Gaudemer O., Huet J.C., and Pernollet J.C. Characterization of elicitin-like phospholipases isolated from Phytophthora capsici culture filtrate. FEBS Letters 452 (1999) 400-406
21. Huet J.-C., Sallé-Tourne M., and Pernollet J.-C. Amino acid sequence and toxicity of the α-elicitin secreted with ubiquitin by Phytophthora infestans. Molecular Plant-Microbe Interactions 7 (1994) 302-304
22. Bonnet P., Bourdon E., Ponchet M., Blein J.P., and Ricci P. Acquired resistance triggered by elicitins in tobacco and other plants. European Journal of Plant Pathology 102 (1996) 181-192
23. Devergne J.C., Fort M.A., Bonnet P., Ricci P., Vergnet C., Delaunay T., et al. Immunodetection of elicitins from Phytophthora spp. using monoclonal antibodies. Plant Pathology 43 (1994) 885-896
24. Kamoun S., Young M., Glascock C., and Tyler B. Extracellular protein elicitors from Phytophthora: host-specificity and induction of resistance to bacterial and fungal phytopathogens. Molecular Plant-Microbe Interactions 6 (1993) 15-25
25. Yu L.M. Elicitins from Phytophthora and basic resistance in tobacco. Proceedings of the National Academy of Sciences of USA 92 (1995) 4088-4094
26. Kamoun S., van West P., Vleeshouwers V.G., de Groot K.E., and Govers F. Resistance of Nicotiana benthamiana to Phytophthora infestans is mediated by the recognition of the elicitor protein INF1. Plant Cell 10 (1998) 1413-1425
27. Kamoun S., Huitema E., and Vleeshouwers V.G.A.A. Resistance to oomycetes: a general role for the hypersensitive response?. Trends in Plant Science 4 (1999) 196-200
28. Colas V., Conrod S., Venard P., Keller H., Ricci P., and Panabiéres F. Elicitin genes expressed in vitro by certain tobacco isolates of Phytophthora parasitica are down regulated during compatible interactions. Molecular Plant-Microbe Interactions 14 (2001) 326-335
29. Brummer M., Arend M., Fromm J., Schlenzig A., and Oβwald W.F. Ultrastructural changes and immunocytochemical localization of the elicitin quercinin in Quercus robur L. roots infected with Phytophthora quercina. Physiological and Molecular Plant Pathology 61 (2002) 109-120
30. Kamoun S. Groovy times: filamentous pathogen effectors revealed. Current Opinion in Plant Biology 10 (2007) 358-365
31. Brasier C.M., Robredo F., and Ferraz J.F.P. Evidence for Phytophthora cinnamomi involvement in Iberian oak decline. Plant Pathology 42 (1993) 140-145
32. Moreira-Marcelino ACM. Aspectos da interacção entre Phytophthora cinnamomi e a doença do declínio em Q. suber e Q. rotundifolia. PhD Thesis, Universidade do Algarve, Faro, Portugal; 2001.
33. Coelho A.C., Cravador A., Bollen A., Ferraz J.F.P., Moreira A.C., Fauconnier A., et al. Highly specific and sensitive non-radioactive identification of Phytophthora cinnamomi. Mycological Research 101 (1997) 1499-1507
34. Bottin A., Larche L., Villalba F., Gaulin E., Esquerré-Tugayé M.-T., and Rickauer M. Green fluorescent protein (GFP) as gene expression reporter and vital marker for studying development and microbe-plant interaction in the tobacco pathogen Phytophthora parasitica var. nicotianae. FEMS Microbiology Letters 176 (1999) 51-56
35. Judelson H.S., Tyler B.M., and Michelmore R.W. Transformation of the oomycete pathogen Phytophthora infestans. Molecular Plant-Microbe Interactions 4 (1991) 602-607
36. Judelson H.S., Coffey M.D., Arredondo F.R., and Tyler B.M. Transformation of the oomycete pathogen Phytophthora megasperma f. sp. glycinea occurs by DNA integration into single or multiple chromosomes. Current Genetics 23 (1993) 211-218
37. Byrt P., and Grant B.R. Some conditions governing zoospore production in axenic cultures of Phytophthora cinnamomi Rands. Australian Journal of Botany 27 (1979) 103-115
38. Unkles S.E., Moon R.P., Hawkins A.R., Duncan J.M., and Kinghorn J.R. Actin in the oomycetous fungus Phytophthora infestans is the product of several genes. Gene 100 (1991) 105-112
39. Dudler R. The single-copy actin gene of Phytophthora megasperma encodes a protein considerably diverged from any other known actin. Plant Molecular Biology 14 (1990) 415-422
40. Yan H.Z., and Liou R.F. Selection of internal control genes for real time quantitative RT-PCR assay in the oomycete plant pathogen Phytophthora parasitica. Fungal Genetics and Biology 43 (2006) 430-438
41. King M. PhD Thesis, Murdoch University, Australia; 2007.
42. Le Berre J.Y., Panabières F., Ponchet F., Bonnet P., Marais A., and Ricci P. Occurrence of multiple forms of elicitins in Phytophthora cryptogea. Plant Physiology and Biochemistry 32 (1994) 251-258
43. Bustin S.A. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. Journal of Molecular Endocrinology 25 (2000) 169-193
44. Blanco F., and Judelson H. A bZIP transcription factor from Phytophthora interacts with a protein kinase and is required for zoospore motility and plant infection. Molecular Microbiology 56 (2005) 638-648
45. Gaulin E., Jauneau A., Villalba F., Rickauer M., Esquerré-Tugayé M.-T., and Bottin A. The CBEL glycoprotein of Phytophthora parasitica var. nicotianae is involved in cell wall deposition and adhesion to cellulosic substrates. Journal of Cell Science 115 (2002) 4565-4575
46. Latijnhouwers M., and Govers F. A Phytophthora infestans G-protein β subunit is involved in sporangium formation. Eukaryotic Cell 2 (2003) 971-977
47. Latijnhouwers M., Ligterink W., Vleeshouwers V.G., van West P., and Govers F. A G alpha subunit controls zoospore motility and virulence in the potato late blight pathogen Phytophthora infestans. Molecular Microbiology 51 (2004) 925-936
48. Kamoun S. Molecular genetics of pathogenic oomycetes. Eukaryotic Cell 2 (2003) 191-199
49. Judelson H.S., and Tani S. Transgene-induced silencing of the zoosporogenesis-specific NIFC gene cluster of Phytophthora infestans involves chromatin alterations. Eukaryotic Cell 6 (2007) 1200-1209
50. Fleischmann F., Koehl J., Portz R., Beltrame A.B., and Oßwald W. Physiological changes of Fagus sylvatica seedlings infected with Phytophthora citricola and the contribution of its elicitin "Citricolin" to pathogenesis. Plant Biology 7 (2005) 650-658
51. Huitema E., Vleeshouwers V.G.A.A., Cakir C., Kamoun S., and Govers F. Differences in intensity and specificity of hypersensitive response induction in Nicotiana spp by INF1, INF2A and INF2B of Phytophthora infestans. Molecular Plant-Microbe Interactions 18 (2005) 183-193
52. Panabières F., Birch P.R.J., Unkles S.E., Ponchet M., Lacourt I., Venard P., et al. Heterologous expression of a basic elicitin from Phytophthora cryptogea in Phytophthora infestans increases its ability to cause leaf necrosis in tobacco. Microbiology 144 (1998) 3343-3349