Multiplex real-time PCR for detection, identification and quantification of 'Candidatus Liberibacter solanacearum' in potato plants with zebra chip

Journal of Microbiological Methods

Volumn 78, Issue 1, 2009, Pages 59-65

  Li, Wenbin ^a   Abad, Jorge A ^a   French Monar, Ronald D ^b   Rascoe, John ^a   Wen, Aimin ^c   Gudmestad, Neil C ^c   Secor, Gary A ^c   Lee, Ing Ming ^d   Duan, Yongping ^e   Levy, Laurene ^a  

^a UNITED STATES DEPARTMENT OF AGRICULTURE   (United States)

^b Plant Pathology and Microbiology   (United States)

^c NORTH DAKOTA STATE UNIVERSITY   (United States)

^d AGRICULTURAL RESEARCH SERVICE   (United States)

^e U S HORTICULTURAL RESEARCH LABORATORY   (United States)

Author keywords

proteobacteria; Bactericera cockerel; Phloem limited bacterium

Indexed keywords

CYTOCHROME C OXIDASE; DNA 16S; PRIMER DNA;

ARTICLE; BACTERIAL COLONIZATION; BACTERIAL COUNT; BACTERIAL GENOME; BACTERIUM; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; CHEMICAL REACTION; CITRUS FRUIT; CONTROLLED STUDY; DNA EXTRACTION; DNA TEMPLATE; HOST; LIBERIBACTER SOLANACEARUM; MOLECULAR PROBE; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT ROOT; PLANT TISSUE; POTATO; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION;

DNA, BACTERIAL; DNA, RIBOSOMAL; MOLECULAR SEQUENCE DATA; PLANT DISEASES; PLANTS; POLYMERASE CHAIN REACTION; RHIZOBIACEAE; RNA, RIBOSOMAL, 16S; SOLANUM TUBEROSUM;

ALPHAPROTEOBACTERIA; BACTERIA (MICROORGANISMS); BACTERICERA; CANDIDATUS LIBERIBACTER; CAPSICUM; CITRUS; EQUUS SUBG. HIPPOTIGRIS; LYCOPERSICON ESCULENTUM; PSYLLIDAE; SOLANUM TUBEROSUM;

EID: 67349280902     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2009.04.009     Document Type: Article

References (29)

1. Abad J.A., Bandla M., French-Monar R.D., Liefting L.W., and Clover G.R.G. First report of the detection of 'Candidatus Liberibacter' species in zebra chip (ZC) disease infected potato plants in the United States. Plant Dis. 93 (2009) 108
2. Bech R.A. Candidatus Liberibacter spp. as putative causal agent of zebra chip of potatoes. USDA-APHIS, SPRO DA 2008-32 (2008) July 14, 2008
3. Davis R.E., Whutecomb R.F., Chen T.A., and Granados R.R. Current status of the aetiology of corn stunt disease. Pathogens Mycoplasmas (1972), Elsevier, Amsterdam
4. Duan, Y., Zhou, L., Hall, D.G., Li, W., Doddapaneni, H., Lin, H., Liu, L., Gabriel, D.W., Vahling, C.M., Williams, K., Dickerman, A., and Tim Gottwald, T. in press. Complete genome sequence of citrus huanglongbing bacterium, 'Candidatus Liberibacter asiaticus' obtained through metagenomics. Mol. Plant-Microbe Interact.
5. Garzón T.J.A., Beverra F.A., Marín A., Mejía A.C.Y., and Byerly M.K.F. Manejo integrado de la enfermedad « Permanente del tomate » (Lycopersicon lycopersicum Karst ex FawII Mill.), en el Bajío. In: Urías C., Rodríguez R., and y Alejandre T. (Eds). Afidos como vectores de virus em México (1992), Colegio de Postgraduados, Centro de Pitopatología Vol
6. Gudmestad N.C., and Secor G.A. Zebra chip: a new disease of potato. Nebr. Potato Eyes 19 1 (2007) 1-4
7. Hansen A.K., Trumble J.T., Stouthamer R., and Paine T.D. New Huanglongbing (HLB) Candidatus species, 'C. Liberibacter solanacearum', found to infect tomato and potato is vectored by the psyllid Bactericerca cockerelli (Sulc). Appl. Environ. Microbiol. (2008) 5862-5865
8. Heid C.A., Stevens J.M., Livak K.L., and Williams P.M. Real-time quantitative PCR. Genome Res. 6 (1996) 986-994
9. Jagoueix S., Bové J.M., and Garnier M. PCR detection of the two 'Candidatus Liberobacter species' associated with greening disease of citrus. Mol. Cell. Probes 10 (1996) 43-50
10. Lee I.-M., Bottner K.D., Munyaneza J.E., Secor G.A., and Gudmestad N.C. Clover proliferation group (16SrVI) subgroup A (16SrVI-A) phytoplasma is a probable causal agent of potato purple top disease in Washington and Oregon. Plant Dis. 88 (2004) 429
11. Lee I.-M., Bottner K.D., Secor G., and Rivera-Varas V. 'Candidatus Phytoplasma americanum', a phytoplasma associated with a potato purple top wilt disease complex. Int. J. Syst. Evol. Microbiol. 56 (2006) 1593-1597
12. Leyva-López N.E., Ochoa-Sábchez J.C., Leal-Klevezas D.S., and Martínez-Soriano J.P. Multiple phytoplasmas associated with potato diseases in Mexico. Can. J. Microbiol. 48 (2002) 1062-1068
13. Li W., Hartung J.S., and Levy L. Quantitative real-time PCR for detection and identification of 'Candidatus Liberibacter species' associated with citrus huanglongbing. J. Microbiol. Methods 66 (2006) 104-115
14. Li W., Hartung J.S., and Levy L. Evaluation of DNA amplification methods for improved detection of 'Candidatus Liberibacter species' associated with citrus huanglongbing. Plant Dis. 91 (2007) 51-58
15. Li W., Li D., Twieg E., Hartung J.S., and Levy L. Optimized quantification of unculturable 'Candidatus Liberibacter sp.' in host plants using real-time PCR. Plant Dis. 92 (2008) 854-861
16. Li W., Abad J.A., and Levy L. C'andidatus Liberibacter solanacearum' associated with zebra chip of potato is not associated with citrus huanglongbing and is absent in Asian citrus psyllids. Proc. of Int. Research Conf. on Huanglongbing (2008), Orland, FL 168
17. Li W., Levy L., and Hartung J.S. Quantitative distribution of 'Candidatus Liberibacter asiaticus' in citrus plants with citrus huanglongbing. Phytopythology 99 (2009) 139-144
18. Liefting L.W., Sutherland P.W., Ward L.I., Paice K.L., Weir B.S., and Clover G.R.G. A new 'Candidatus Liberibacter' species associated with Solanaceous crops. Plant Dis. 92 (2008) 1588
19. Liefting L.W., Sutherland P.W., Ward L.I., Paice K.L., Weir B.S., and Clover G.R.G. A new 'Candidatus Liberibacter' species associated with diseases of solanacerous crops. Plant Dis. 93 (2009) 208-214
20. Liefting, L.W., Weir, B.S., Pennycook, S.R., and Clover, G.R.G. in press. 'Candidatus Liberibacter solanacearum', a liberibacter associated with plants in the family Solanaceae. Int. J. Syst. Evol. Microbiol.
21. Lin H., Doddapaneni H., Munyaneza J.E., Civerolo E.L., Sengoda V.G., Buchman J.L., and Stenger D.C. Molecular characterization and phylogenetic analysis of 16S rRNA from a new 'Candidatus Liberibacter' strain associated with zebra chip of potato (Solanum tuberosum L.) and the potato psyllid (Bactericera cockerelli Sulc.). J. Plant Pathol. 91 (2009) 215-219
22. Montero-Astua M., Hartung J.S., Aguilar E., Chacon C., Li W., Albertazzi F.J., and Rivera C. Genetic diversity of Xylella fastidiosa from Costa Rica, São Paulo and United States of America. Phytopathology 97 (2007) 1338-1347
23. Munyaneza J.Z., Crosslin J.M., and Upton J.E. Association of Bactericera cockerelli (Homoptera Psyllidae) with zebra chip, a new potato disease in Southwestern United States and Mexico. J. Econ. Entomol. 100 (2007) 656-663
24. Palaniappan R.U.M., Chang Y.F., Chang C.F., Pan M.J., Yang C.W., Harpending P., McDonouhg S.P., Dubovi E., Divers T., Qu J., and Roe B. Evaluation of lig-based conventional and real-time PCR for detection of pathogenic leptospires. Mol. Cell. Probes 19 (2005) 111-117
25. Richard B.L. A new and destructive disease of the potato in Utah and its relation to potato psylla. Phytopathology 18 (1928) 140-141
26. Secor G.A., and Rivera V.V. Emerging diseases of cultivated potato and their impact on Latin America. Rev. Latinoam. Papa 1 (2004) 1-8 (Suppl.)
27. Secor G.A., Rivera V.V., Rengifo J., De Boer S.H., Lee I.-M., and Gudmestad N.C. Zebra chip: a new disease of potato. Am. J. Potato Res. 85 (2008) 29
28. Teixeira D.C., Ayres A.J., Kitajima E.W., Danet L., Jagoueix-Eveillard S., Saillard C., and Bové J.M. First report of a huanglongbing-like disease of citrus in São Paulo, Brazil, and association of a new Liberibacter species, 'Candidatus Liberibacter americanus' with the disease. Plant Dis. 89 (2005) 107
29. Weisburg W.G., Barns S.M., Pelletier D.A., and Lane D.J. 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173 (1991) 697-703