An easy ratiometric compensation for the extracellular Ca2+ indicator-caused fluorescence artifact

Analytical Biochemistry

Volumn 390, Issue 2, 2009, Pages 212-214

  Kukkonen, Jyrki P ^a,b  

^a UNIVERSITY OF HELSINKI   (Finland)

^b UPPSALA UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

CALIBRATION;

CALIBRATION PROTOCOLS; EXTRACELLULAR; FLUORESCENCE SPECTROPHOTOMETER; INTERNAL CALIBRATION; INTRACELLULAR CA; RATIOMETRIC; REAL-TIME ASSAYS; SINGLE-STEP;

FLUORESCENCE;

CALCIUM ION;

ARTICLE; ARTIFACT; CALIBRATION; EXTRACELLULAR CALCIUM; FLUORESCENCE ANALYSIS; MATHEMATICAL ANALYSIS; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; SPECTROPHOTOMETER;

EID: 67349274521     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.04.017     Document Type: Article

References (3)

1. 2+ indicators with greatly improved fluorescence properties. J. Biol. Chem. 260 (1985) 3440-3450
2. Di Virgilio F., Steinberg T.H., and Silverstein S.C. Inhibition of fura-2 sequestration and secretion with organic anion transport blockers. Cell Calcium 11 (1990) 57-62
3. 2+ imaging in single living cells: theoretical and practical issues. Cell Calcium 11 (1990) 157-179