p16, Ki-67, and BD ProEx™C immunostaining: a practical approach for diagnosis of cervical intraepithelial neoplasia

Human Pathology

Volumn 40, Issue 7, 2009, Pages 957-964

  Walts, Ann E ^a   Bose, Shikha ^a  

^a Cedars Sinai Medical Center   (United States)

Author keywords

Cervical intraepithelial neoplasia (CIN); Ki 67; p16; ProEx C

Indexed keywords

KI 67 ANTIGEN; PROTEIN P16;

ADULT; AGED; ARTICLE; CANCER DIAGNOSIS; CANCER GRADING; CANCER RISK; DIAGNOSTIC ACCURACY; FEMALE; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; MAJOR CLINICAL STUDY; PRACTICE GUIDELINE; SENSITIVITY AND SPECIFICITY; UTERINE CERVIX CARCINOMA IN SITU; WART VIRUS;

ADULT; AGED; AGED, 80 AND OVER; CERVICAL INTRAEPITHELIAL NEOPLASIA; CYCLIN-DEPENDENT KINASE INHIBITOR P16; FEMALE; HUMANS; IMMUNOHISTOCHEMISTRY; INDICATORS AND REAGENTS; KI-67 ANTIGEN; MIDDLE AGED; SENSITIVITY AND SPECIFICITY; STAINING AND LABELING;

EID: 67349113948     PISSN: 00468177     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.humpath.2008.12.005     Document Type: Article

References (23)

1. Hu L., Guo M., He Z., Thornton J., McDaniel L.S., and Hughson M.D. Human papillomavirus genotyping and p16INK4a expression in cervical intraepithelial neoplasia of adolescents. Mod Pathol 18 (2005) 267-273
2. Kelly D., Kincaid E., Fansler Z., Rosenthal D.L., and Clark D.P. Detection of cervical high-grade squamous intraepithelial lesions from cytologic samples using a novel immunocytochemical assay (ProEx C). Cancer 108 (2006) 494-500
3. Klaes R., Benner A., Friedrich T., et al. p16INK4a immunohistochemistry improves interobserver agreement in the diagnosis of cervical intraepithelial neoplasia. Am J Surg Pathol 26 (2002) 1389-1399
4. O'Neill C.J., and McCluggage W.G. P16 expression in the female genital tract and its value in diagnosis. Adv Anat Pathol 13 (2006) 8-15
5. Sano T., Masuda N., Oyama T., and Nakajima T. Overexpression of p16 and p14ARF is associated with human papillomavirus infection in cervical squamous cell carcinoma and dysplasia. Pathol Int 52 (2002) 375-383
6. Ortega S., Malumbres M., and Barbacid M. Cyclin D-dependent kinases INK4 inhibitors and cancer. Biochim Biophys Acta 1602 (2002) 73-87
7. Indinnemo M., Cicchini C., Stazi A., et al. Immunohistochemical assessment of Ki-67 as prognostic cellular proliferation marker in anal canal carcinoma. J Exp Clin Cancer Res 19 (2000) 471-475
8. Chen Y., Miller C., Mosher R., et al. Identification of cervical cancer markers by cDNA and tissue microarrays. Cancer Res 63 (2003) 1927-1935
9. Malinowski D.P. Multiple biomarkers in molecular oncology. I. Molecular diagnostics applications in cervical cancer detection. Expert Rev Mol Diagn 7 (2007) 117-131
10. Santin A.D., Zhan F., Bignotti E., et al. Gene expression profiles of primary HPV 16- and HPV 18-infected early stage cervical cancers and normal cervical epithelium: identification of novel candidate molecular markers for cervical cancer diagnosis and therapy. Virology 331 (2005) 269-291
11. Badr R.E., Walts A.E., Chung F., and Bose S. BD ProEx C: a sensitive and specific marker of HPV-associated squamous lesions of the cervix. Am J Surg Pathol 32 (2008) 899-906
12. Klaes R., Friedrich T., Spitkovsky D., et al. Overexpression of p16(INK4A) as a specific marker for dysplastic and neoplastic epithelial cells of the cervix uteri. Int J Cancer 92 (2001) 276-284
13. Sano T., Oyama T., Kashiwabara K., Fukuda T., and Nakajima T. Expression status of p16 protein is associated with human papillomavirus oncogenic potential in cervical and genital lesions. Am J Pathol 153 (1998) 1741-1748
14. Pinto A.P., Schlecht N.F., Woo T.Y.C., Crum C.P., and Cibas E.S. Biomarker (ProEx™C, p16(INK4A), and MiB-1) distinction of high-grade squamous intraepithelial lesion from its mimics. Mod Pathol 21 (2008) 1067-1074
15. Redman R., Rufforny I., Liu C., Wilkinson E.J., and Massoll N.A. The utility of p16(Ink4a) in discriminating between cervical intraepithelial neoplasia 1 and nonneoplastic equivocal lesions of the cervix. Arch Pathol Lab Med 132 (2008) 795-799
16. Shi J., Liu H., Wilkerson M., et al. Evaluation of p16INK4a, minichromosome maintenance protein 2, DNA topoisomerase IIalpha, ProEX C, and p16INK4a/ProEX C in cervical squamous intraepithelial lesions. Hum Pathol 38 (2007) 1335-1344
17. Walts A.E., and Bose S. P16/Ki-67 immunostaining is useful in stratification of atypical metaplastic epithelium of the cervix. Clinical Medicine: Pathology 1 (2008) 35-42 [Electronic publication]
18. Iaconis L., Hyjek E., Ellenson L.H., and Pirog E.C. p16 and Ki-67 immunostaining in atypical immature squamous metaplasia of the uterine cervix: correlation with human papillomavirus detection. Arch Pathol Med 131 (2007) 1343-1349
19. Wong A.K., Chan R.C.K., Nichols W.S., et al. Human papillomavirus (HPV) in atypical squamous cervical cytology: the Invader HPV Test as a new screening assay. J Clin Microbiol 46 (2008) 869-875
20. Walts A.E., Lechago J., and Bose S. P16 and Ki-67 immunostaining is a useful adjunct in the assessment of biopsies for HPV-associated anal intraepithelial neoplasia. Am J Surg Pathol 30 (2006) 795-801
21. Agoff S.N., Lin P., Morihara J., Mao C., Kiviat N.B., and Koutsky L.A. p16(INK4a) expression correlates with degree of cervical neoplasia: a comparison with Ki-67 expression and detection of high-risk HPV types. Mod Pathol 16 (2003) 665-673
22. Van Niekerk D., Guillaud M., Matisic J., et al. p16 and MIB1 improve the sensitivity and specificity of the diagnosis of high grade squamous intraepithelial lesions: methodological issues in a report of 447 biopsies with consensus diagnosis and HPV HCII testing. Gynecol Oncol 107 1 Suppl 1 (2007) S233-S240 [Electronic publication 2007 Sep 7]
23. Qiao X., Bhuiya T.A., and Spitzer M. Differentiating high-grade cervical intraepithelial lesion from atrophy in postmenopausal women using Ki-67, cyclin E, and p16 immunohistochemical analysis. J Low Genit Tract Dis 9 (2005) 100-107