Efficient screening for new amino acid dehydrogenase activity: Directed evolution of Bacillus sphaericus phenylalanine dehydrogenase towards activity with an unsaturated non-natural amino acid

Journal of Biotechnology

Volumn 142, Issue 2, 2009, Pages 127-134

  Chen, Sihong ^a   Engel, Paul C ^a  

^a UNIVERSITY COLLEGE DUBLIN   (Ireland)

Author keywords

Biocatalysis; Directed evolution; Error prone PCR; High throughput screening; Parallel over expression; Phenylalanine dehydrogenase

Indexed keywords

BIOCATALYSIS; DIRECTED EVOLUTION; ERROR-PRONE PCR; HIGH-THROUGHPUT SCREENING; PARALLEL OVER-EXPRESSION; PHENYLALANINE DEHYDROGENASE;

AMINATION; AMINES; BACTERIOLOGY; ENZYMES; MUTAGENESIS; ORGANIC ACIDS; TARGETS; THROUGHPUT;

AMINO ACIDS;

OXIDOREDUCTASE; PHENYLALANINE DEHYDROGENASE; PROPARGYLGLYCINE; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS SPHAERICUS; BACTERIAL GENETICS; BACTERIUM MUTANT; CATALYSIS; CONTROLLED STUDY; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME SUBSTRATE; GENE MUTATION; HIGH THROUGHPUT SCREENING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ALKYNES; AMINO ACID OXIDOREDUCTASES; AMINO ACID SEQUENCE; BACILLUS; BIOCATALYSIS; CATALYTIC DOMAIN; DIRECTED MOLECULAR EVOLUTION; GLYCINE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS; POLYMERASE CHAIN REACTION; PROTEIN CONFORMATION; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY;

BACILLUS SPHAERICUS;

EID: 67349084648     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2009.03.005     Document Type: Article

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