Detection of methicillin-resistant staphylococcus aureus (MRSA) using the nanolantern™ biosensor

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Volumn 7167, Issue , 2009, Pages

  Strohsahl, Christopher M ^a,b   Miller, Benjamin L ^a,b   Krauss, Todd D ^b  

^a Lighthouse Biosciences LLC   (United States)

^b UNIVERSITY OF ROCHESTER   (United States)

Author keywords

Arrayable; Detection; Dna; Methicillin resistance; Staphylococcus aureus

Indexed keywords

ANTIBIOTIC RESISTANCE; ARRAYABLE; BACTERIAL INFECTIONS; DETECTION; DNA DETECTION; DNA HAIRPIN; GENE ANALYSIS; LABEL-FREE SENSORS; METHICILLIN; METHICILLIN-RESISTANCE; METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS; PCR ASSAY; RAPID DETECTION; S. AUREUS; SEQUENCE SPECIFICITY; STAPHYLOCOCCUS AUREUS;

ANTIBIOTICS; BIOASSAY; BIOCHIPS; BIOSENSORS; DNA; GENES; NANOSENSORS; PROBES;

NUCLEIC ACIDS;

EID: 67249121790     PISSN: 16057422     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.808872     Document Type: Conference Paper

References (42)

1. Lowy, F.D., "Medical Progress: Staphylococcus aureus Infections." N. Engl. J. Med. 339, 520-532 (1998).
2. Wenzel, R.P., Edmond, M.B., "The Impact of Hospital-Acquired Bloodstream Infections." Emerg. Infect. Dis. 7, 174-177 (2001).
3. Rubin, R.J., Harrington, CA., Poon, A., Dietrich, K., Greene, J.A., Moiduddin, A., "The Economic Impact of Staphylococcus aureus Infection in New York City Hospitals." Emerg. Infect. Dis. 5, 9-17 (1999).
4. Chambers, H.F., "The Changing Epidemiology of Staphylococcus aureus?" Emerg. Infect. Dis. 7, 178-182 (2001).
5. No author listed, "National Nosocomial Infections Surveillance System report." Am. J. Infect. Control 31, 481 (2003).
6. Warren, D.K., Liao, R.S., Merz, L.R., Eveland, M., Dunne, Jr., W.M., "Detection of Methicillin-Resistant Staphylococcus aureus Directly from Nasal Swab Specimens by a Real-Time PCR Assay" J. Clin. Microbiol. 42, 5578-5781 (2004).
7. Miller, L.G., Perdreau-Reminton, F., Rieg, G., Mehdi, S., Perlroth, J., Bayer, A.S., Tang, A.W., Phung, T.O., Spellberg, B., "Necrotizing Fasciitis Caused by Community-Associated Methicillin-Resistant Staphylococcus aureus in Los Angles" N. Engl. J. Med. 352, 1445-1453 (2005).
8. Chaix, C., Durand-Zaleski, I., Alberti, C., Brun-Buisson, C., "Control of Endemic Methicillin-Resistant Staphylococcus aureus" JAMA 282, 1745-1751 (1999).
9. No author listed, "New Research Estimates MRSA Infections Cost U.S. Hospitals $3.2 Billion to $4.2 Billion Annually" Infection Control Today. http://www.infectioncontroltoday.com/hotnews/55hl68584264313,p3. html#, (2005).
10. Gorwitz, R.J., Jernigan, D.B., Powers, J.H., Jernigan, JA., and Participants in the CDC Convened Experts' Meeting on Management of MRSA in the Community. "Strategies for clinical management of MRSA in the community: Summary of an experts' meeting convened by the Centers for Disease Control and Prevention." http://www.cdc.gov/ncidod/dhqp/ar-mrsa-ca.html., (2006).
11. Chambers, H.F., "Methicillin Resistance in Staphylococci: Molecular and Biochemical Basis and Clinical Implications." Clin. Microbiol. Rev. 10, 781-791 (1997).
12. Safdar, N., Narans, L., Gordon, B., Maki, D., "Comparison of Culture Screening Methods for Detection of Nasal Carriage of Methicillin-Resistant Staphylococcus aureus: a Prospective Study Comparing 32 Methods." J. Clin. Microbiol. 41, 3163-3166 (2003).
13. Du, H., Disney, M.D., Miller, B.L., Krauss, T.D., "Hybridization- Based Unquenching of DNA Hairpins on Au Surfaces: Prototypical 'Molecular Beacon' Biosensors" J. Am. Chem. Soc. 125, 4012-4013 (2003).
14. Warren, D.K., Liao, R.S., Merz, L.R., Eveland, M., Dunne, Jr., W.M., "Detection of Methicillin-Resistant Staphylococcus aureus Directly from Nasal Swab Specimens by a Real-Time PCR Assay." J. Clin. Microbiol. 42, 5578-5781 (2004).
15. Jonas, D., Speck, M., Dachner, F.D., Grundmann, H., "Rapid PCR-Based Identification of Methicillin-Resistant Staphylococcus aureus from Screening Swabs." J. Clin. Microbiol. 40, 1821-1823 (2002).
16. Vet, J.A.M., Majithia, A.R., Marras, S.A.E., Tyagi, S., Dube, S., Poiesz, B.J., Kramer, F.R., "Multiplex Detection of Four Pathogenic Retroviruses Using Molecular beacons." Proc. Natl. Acad. Sci. 96, 6394-6399 (1999).
17. Chen, W., Martinez, G., Mulchandani, A., "Molecular Beacons: a Real-Time Polymerase Chain Reaction Assay for Detecting Salmonella." Anal. Biochem. 280, 166-172, (2000).
18. Elsayed, S., Chow, B.L., Hamilton, N.L., Gregson, D.B., Pitout, J.D., Church, D.L., "Development and Validation of a Molecular Beacon Probe-Based Real-Time Polymerase Chain Reaction Assay for Rapid Detection of Methicillin Resistance in Staphylococcus aureus." Arch. Pathol. Lab. Med. 127, 845-849, (2003).
19. Fortin, NY., Mulchandani, A., Chen, W., "Use of Real-Time Polymerase Chain Reaction and Molecular Beacons for the Detection of Escherichia coli O157:H7." Anal. Biochem. 289, 281-288 (2001).
20. O'Shea, M.K., Cane, P.A., "Development of a Highly Sensitive Semi-quantitative Real-Time PCR and Molecular Beacon Probe Assay for the Detection of Respiratory Syncytial Virus." J. Virol. Methods 118, 101-110 (2004).
21. Ramachandran, A., Flinchbaugh, J., Ayoubi, P., Olah, G.A., Malayer, J.R., "Target Discrimination by Surface-Immobilized Molecular Beacons Designed to Detect Francisella tularensis." Biosensors and Bioelectronics 19, 727-736 (2004).
22. Fan, J., Yang, X., Wang, W., Wood III, W.H., Becker, K.G., Gorospe, M., "Global Analysis of Stress-Regulated mRNA Turnover by Using cDNA Arrays." Proc. Natl. Acad. Sci. 99, 10611-10616 (2002).
23. Fang, X., Liu, L., Schuster, S., Tan, W., "Designing a Novel Molecular Beacon for Surface-Immobilized DNA Hybridization Studies." J. Am. Chem. Soc. 121, 2921-2922, (1999).
24. Strohsahl, CM., Krauss, T.D., Miller, B.L., "Identification of high-stringency DNA hairpinprobes by partial gene folding." Biosenors and Bioelectronics, 23, 233-240 (2007).
25. Clarke, S.R., Harris, L.G., Richards, R.G., Foster, S.J., "Analysis of Ebh, a 1.1-Megadalton Cell Wall-Associated Fibronectin-Binding Protein of Staphylococcus aureus." Infect. Immun. 70, 6680-6687 (2002).
26. Du, H., Strohsahl, CM., Camera, J., Miller, B.L., Krauss, T.D., "Sensitivity and Selectivity of Metal Surface-Immobilized 'Molecular Beacon' Biosensors" J. Am. Chem. Soc. 127, 7932-7940 (2005).
27. Strohsahl, CM., Du, H.; Miller, B.L., Krauss, T.D., "Towards single-spot multianalyte molecular beacon biosensors." Talanta 67, 479-485 (2005).
28. Archer, G.L., Niemeyer, D.M., Thanassi, J.A., Pucci, M.J., "Dissemination Among Staphylococci of DNA Sequences Associated with Methicillin Resistance." Antimicrob. Agents Chemother. 38, 447-454 (1994).
29. Kuroda, M., Ohta, T., Uchiyama, I., Baba, T., Yuzawa, H., Kobayashi, I., Cui, L., Oguchi, A., Aoki, K.-L, Nagai, Y., Lian, J.Q., Ito, T., Kanamori, M., Matsumaru, H., Maruyama, A., Murakami, H., Hosoyama, A., Mizutani-Ui, Y., Takahashi, N.K., Sawano, T., Inoue, R.-L, Kaito, C., Sekimizu, K., Hirakawa, A., Kuhara, S., Goto, S., Yabuzaki, J., Kanehisa, M., Yamashita, A., Oshima, K., Furuya, K., Yoshino, C., Shiba, T., Hattori, M., Ogasawara, N., Hayashi, H., Hiramatsu, K., "Whole Genome Sequencing of Meticillin-Resistant Staphylococcus aureus." Lancet 357, 1225-1240 (2001).
30. National Center for Biotechnology Information: http://www.ncbi.nlm.nih. gov.
31. Mathews, D.H., Sabina, J., Zuker, M., Turner, D.H., "Expanded Sequence Dependence of Thermodynamic Parameters Improves Prediction of RNA Secondary Structure." J. Mol. Biol. 289, 911-940 (1999).
32. Mathews, D.H., Disney, M.D., Childs, J.L., Schroeder, S.J., Zuker, M., Turner, D.H., "Incorporating Chemical Modification Constraints into a Dynamic Programming Algorithm for Prediction of RNA Secondary Structure." Proc. Natl. Acad. Sci. 101, 7287-7792 (2004).
33. Adapted from protocol found at: www.microarrays.org/pdfs/Total-RNA-from- Ecoli.pdf
34. Staphylococcus aureus subsp. aureus Serological type (b)cl ATCC® 27217
35. Staphylococcus aureus subsp. aureus ATCC® 43300
36. Rozen S. and Skaletsky, HJ., [Bioinformatics Methods and Protocols: Methods in Molecular Biology.] Humana Press, Totowa, NJ, 365-386 (2000).
37. D.H. Mathews, "Personal communication"
38. Altchul, S.F., Gish, W., Miller, W., Meyers, E.W., Lipman, DJ., "Basic Local Alignment Search Tool." J. Mol. Biol. 215, 403-410 (1990).
39. Tsourkas, A., Behlke, M.A., Rose, S.D., Bao, G., "Hybridization Kinetics and Thermodynamics of Molecular Beacons." Nucleic Acids Res. 31, 1319-1330 (2003).
40. Peterson, A.W., Wolf, L.K., Georgiadis, R.M., "Hybridization of Mismatched or Partially Matched DNA at Surfaces." J. Am. Chem. Soc. 124, 14601-14607, (2002).
41. Ennifar, E., Walter, P., Ehresmann, B., Ehresmann, C., Dumas, P., "Crystal Structures of Coaxially Stacked Kissing Complexes of the HIV-1 RNA Dimerization Initiation Site." Nature Struct. Biol. 8, 1064-1068 (2001).
42. Tyagi, S., Kramer, F.R., "Molecular Beacons: Probes that Fluoresce upon Hybridization." Nature Biotech. 14, 303-308 (1996).