High-quality plant DNA extraction for PCR: An easy approach

Journal of Applied Genetics

Volumn 50, Issue 2, 2009, Pages 105-107

  Ahmed, I ^a,b   Islam, M ^a,c   Arshad, W ^a   Mannan, A ^a   Mirza, B ^a  

^a QUAID I AZAM UNIVERSITY   (Pakistan)

^b MASSEY UNIVERSITY   (New Zealand)

^c HAZARA UNIVERSITY   (Pakistan)

Author keywords

Chloroform; DNA extraction; Hydrated ether; Isoamyl alcohol; Microsatellites; Phenol; Plant tissues; Polysaccharides

Indexed keywords

HORDEUM; LYCOPERSICON ESCULENTUM; SOLANUM TUBEROSUM;

EID: 67149107218     PISSN: 12341983     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF03195661     Document Type: Article

References (12)

1. Cheng YJ, Guo WW, Yi HL, Pang XM, Deng X, 2003. An efficient protocol for genomic DNA extraction from citrus species. Plant Mol Biol Rep 21: 177a-177g.
2. Doyle JJ, Doyle JL, 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19: 11-15.
3. Fang G, Hammar S, Grumet RA, 1992. A quick and inexpensive method for removing polysaccharides from plant genomic DNA. Biofeedback 13: 52-54.
4. Hill-Ambroz KL, Brown-Guedira GL, Fellers JP, 2002. Modified rapid DNA extraction protocol for high throughput microsatellite analysis in wheat. Crop Sci 42: 2088-2091.
5. Huang X, Zeller FJ, Hsam SLK, Wenzel G, Mohler V, 2000. Chromosomal location of AFLP markers in common wheat, utilizing nullitetrasomic stocks. Genome 43: 298-305.
6. Jobes DV, Hurley DL, Thien LB, 1995. Plant DNA isolation: a method to efficiently remove polyphenolics, polysaccharides, and RNA. Taxon 44: 379-386.
7. Kang TJ, Yang MS, 2004. Rapid and reliable extraction of genomic DNA from various wild-type and transgenic plants. BMC Biotechnol. doi: 10.1186/1472-6750-4-20.
8. Lodhi MA, Ye GN, Weeden NF, Reisch BI, 1994. A simple and efficient method for DNA extractions from grapevine cultivars and Vitis species and Ampelopsis. Plant Mol Biol Rep 12: 6-13.
9. Mogg RJ, Bond JM, 2003. A cheap, reliable and rapid method of extracting high-quality DNA from plants. Mol Ecol Note 3: 666-668.
10. Porebski S, Bailey LG, Baum BR, 1997. Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol Biol Rep 15: 8-15.
11. Schlink K, Reski R, 2002. Preparing high-quality DNA from moss (Physcomitrella patens). Plant Mol Biol Rep 20: 423a-423f.
12. Sharma AD, Gill PK, Singh P, 2002. DNA isolation from dry and fresh samples of polysaccharide-rich plants. Plant Mol Biol Rep 20: 415a-415f.