Synthesis of zwitterionic stationary phase based on hydrophilic non-porous poly(glycidymethacrylate-coethylenedimethacrylate) beads and their application for fast separation of proteins

Journal of Applied Polymer Science

Volumn 113, Issue 2, 2009, Pages 984-991

  Gong, Bolin ^a,b   Bo, Chunmiao ^a   Zhu, Jinxia ^a   Yan, Chao ^b  

^a NINGXIA UNIVERSITY   (China)

^b Unimicro Shanghai Technologies Co Ltd   (China)

Author keywords

Monodisperse non porous polymeric beads; Protein separation; Zwitterionic stationary phase

Indexed keywords

BOVIN SERUM ALBUMINS; COLUMN LOADING; CRUDE EXTRACT; DIMETHYLAMINE; DYNAMIC PROTEINS; FAST SEPARATION; GLYCIDYL METHACRYLATE; ION EXCHANGE PROPERTIES; MONODISPERSE; MONODISPERSE NON-POROUS POLYMERIC BEADS; PROTEIN SEPARATION; RAPID SEPARATION; RECOMBINANT HUMAN INTERFERON; REPRODUCIBILITY; STATIONARY PHASE; ZWITTERIONIC STATIONARY PHASE;

CHEMICAL MODIFICATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH PRESSURE LIQUID CHROMATOGRAPHY; HYDROPHILICITY; INTERFERONS; ION EXCHANGE; PH EFFECTS; POLYMERS; RESINS; SEPARATION; SYNTHESIS (CHEMICAL);

CHROMATOGRAPHIC ANALYSIS;

BEAD; HYDROPHILIC PROPERTY; ION EXCHANGE; PROOFING; PROTEIN; PURIFICATION; SYNTHESIS;

EID: 67149096057     PISSN: 00218995     EISSN: 10974628     Source Type: Journal    
DOI: 10.1002/app.29743     Document Type: Article

References (21)

1. Nesterenko, P. N.; Hadded, P. R. Anal Sci 2000, 16, 565.
2. Knox, J. H.; Jurand, J. J Chromatogr 1981, 203, 85.
3. Yu, L. W.; Floyd, T. R.; Hartwick, R. A. J Chromatogr Sci 1986, 24, 177.
4. Yu, L. W.; Hartwick, R. A. J Chromatogr Sci 1989, 27, 176.
5. Hu, W. Z.; Takeuchi, T.; Haraguchi, H. Anal Chem 1993, 65, 2204.
6. Hu, W. Z.; Tao, H.; Haraguchi, H. Anal Chem 1994, 65, 2514.
7. Hu, W. Z.; Tominaga, M.; Cao, S. A.; Miyazaki, A. Anal Chim Acta 1994, 299, 249.
8. Hu, W. Z.; Tanaka, K.; Haddad, P. R.; Hasebe, K. J Chromatogr A 2000, 884, 161.
9. Jiang, W.; Irgum, K. Anal Chem 1999, 71, 333.
10. Jiang, W.; Irgum, K. Anal Chem 2002, 74, 4682.
11. Fu, H. J.; Xie, C. H.; Dong, J.; Zou, H. F. Anal Chem 2004, 76, 4866.
12. Chen, C. H.; Lee, W. C. J Chromatogr A 2001, 921, 31.
13. Lee, W. C; Lin, C. H.; Ruaa, R. C; Hsu, K. Y. J Chromatogr A 1995, 704, 307.
14. Round, M. A.; Regnier, F. E. J Chromatogr 1988, 443, 73.
15. Gong, B. L.; Zhu, J. X.; Li, L.; Qiang, K. J.; Ren, L. Talanta 2006, 68, 666.
16. Zhu, J. X.; Bo, C. M.; Gong, B. L. Chin J Chromatogr (in Chinese) 2006, 24, 129.
17. Bradford, M. M. Anal Biochem 1983, 133, 251.
18. Gao, W. H.; Yang, G. L.; Yang, J.; Liu, H. Y.; Yin, J. F.; Chen, Y. Chin J Anal Chem (in Chinese) 2004, 32, 295.
19. Kato, Y.; Kitamura, T.; Nakatani, S.; Hashimoto, T. J Chromatogr 1989, 483, 401.
20. Mihelic, I.; Koloini, T.; Podgornik, A.; Strancar, A. J High Resolut Chromatogr 2000, 23, 39.
21. Lloyd, L. L. J Chromatogr 1991, 544, 201.