Label-free DNA sensor based on surface charge modulated ionic conductance

ACS Nano

Volumn 3, Issue 4, 2009, Pages 1004-1010

  Wang, Xian ^a   Smirnov, Sergei ^a  

^a NEW MEXICO STATE UNIVERSITY   (United States)

Author keywords

Dna; Ionic conductance; Label free sensor; Morpholino; Nanopores; Surface charge effect

Indexed keywords

DETECTION METHODS; DNA BINDING; DNA SENSORS; IONIC CONDUCTANCE; LABEL FREE; LABEL-FREE SENSOR; MORPHOLINO; NANO-POROUS ALUMINA MEMBRANES; ORDER OF MAGNITUDE; SURFACE CHARGE EFFECT; SURFACE MODIFICATION;

CHARGED PARTICLES; DNA; GENES; LABELS; NANOPORES; SENSORS; SILANES; SURFACE CHARGE;

NUCLEIC ACIDS;

ALUMINUM OXIDE; DNA; NANOMATERIAL;

ARTICLE; CHEMISTRY; ELECTRIC CONDUCTIVITY; GENETIC PROCEDURES; OSMOLARITY; SCANNING ELECTRON MICROSCOPY; SURFACE PROPERTY; ULTRASTRUCTURE;

ALUMINUM OXIDE; BIOSENSING TECHNIQUES; DNA; ELECTRIC CONDUCTIVITY; MICROSCOPY, ELECTRON, SCANNING; NANOSTRUCTURES; OSMOLAR CONCENTRATION; SURFACE PROPERTIES;

EID: 66949136752     PISSN: 19360851     EISSN: 1936086X     Source Type: Journal    
DOI: 10.1021/nn900113x     Document Type: Article

References (18)

1. Vlassiouk, I.; Takmakov, P.; Smirnov, S. Sensing DNA Hybridization via Ionic Conductance through a Nanoporous Electrode. Langmuir 2005, 21, 4776-4778.
2. Takmakov, P.; Vlassiouk, I.; Smirnov, S. Hydrothermally Shrunk Alumina Nanopores and their Application to DNA Sensing. Analyst 2006, 131, 1248-1253.
3. Smirnov, S.; Vlassiouk, I.; Rios, F.; Takmakov, P.; Gust, D. Smart Nanoporous Membranes. ECS Trans. 2007, 3, 23.
4. Vlassiouk, I.; Smirnov, S. In Biosensing Using Nanomaterials; Merkoci, A., Ed.; Wiley: New York, 2009; Chapter 15, pp 459-490, ISBN 978-0-470-18309-0.
5. Stein, D.; Kruithof, M.; Dekker, C. Surface-Charge-Governed Ion Transport in Nanofluidic Channels. Phys. Rev. Lett. 2004, 93, 035901-1-035901-4.
6. Vlassiouk, I.; Smirnov, S. S.; Siwy, Z. Ionic Selectivity of Single Nanochannels. Nano Lett. 2008, 8, 1978-1985.
7. Karnik, R.; Duan, C.; Castelino, K.; Daiguji, H.; Majumdar, A. Rectification of Ionic Current in a Nanofluidic Diode. Nano Lett. 2007, 7, 547-551.
8. Jagerszki, G.; Gyurcsanyi, R. E.; Hofler, L; Pretsch, E. Hybridization-Modulated Ion Fluxes through Peptide-Nucleic-Acid-Functionalized Gold Nanotubes. A New Approach to Quantitative Label-Free DNA Analysis. Nano Lett. 2007, 7, 1609.
9. Vlassiouk, I. Unpublished results.
10. See the Supporting Information
11. Kerem, B.; Rommens, J. M.; Buchanan, J. A.; Markiewicz, D.; Cox, T. K.; Chakravarti, A.; Buchwald, M.; Tsui, L. C. Identification of the Cystic Fibrosis Gene: Genetic Analysis. Science 1989, 245, 1073-1080.
12. Riordan, J. R.; Rommens, J. M.; Kerem, B.; Alon, N.; Rozmahel, R.; Grzelczak, Z.; Zielenski, J.; Lok, S.; Plavsic, N.; Chou, J. L; et al. Identification of the Cystic Fibrosis Gene: Cloning and Characterization of Complementary DNA. Science 1989, 245, 1066-1073.
13. Rommens, J. M.; Iannuzzi, M. C.; Kerem, B.; Drumm, M. L; Melmer, G.; Dean, M.; Rozmahel, R.; Cole, J. L; Kennedy, D.; Hidaka, N.; et al. Identification of the Cystic Fibrosis Gene: Chromosome Walking and Jumping. Science 1989, 245, 1059-1065.
14. Chen, E. Y.; Bartlett, M. C.; Loo, T. W.; Clarke, D. M. The AF508 Mutation Disrupts Packing of the Transmembrane Segments of the Cystic Fibrosis Transmembrane Conductance Regulator. J. Biol. Chem. 2004, 279, 39620-39627.
15. Szczepanski, V.; Vlassiouk, I. S.; Smirnov, S. Stability of Silane Modifiers on Alumina Nanoporous Membranes. J. Membr. Sci. 2006, 281, 587-591.
16. Brown, G. E.; Henrich, V. E.; Casey, W. H.; Clark, D. L.; Eggleston, C.; Felmy, A.; Goodman, D. W.; Gratzel, M.; Maciel, G.; McCarthy, M. I.; et al. Met al. Oxide Surfaces and Their Interactions with Aqueous Solutions and Microbial Organisms. Chem. Rev. 1999, 99, 77-174.
17. 0εζ/ηλ, arises from electrolyte moving in the pore under the pressure gradient, ΔP, and gains the value, ΔV, proportional to the surface charge density (the surface zeta potential, ζ), where ε and η are the solvent dielectric constant and viscosity, respectively. The value decreases with increasing conductivity of the electrolyte, λ; we used 10 μM KCl. The exact correlation between ΔV and the surface charge density given in the formula depends on the velocity profile of the liquid near the channel walls. We have found that even though the streaming potential is convenient in identifying the sign of the surface charge, e.g., the values are opposite for alumina and when its surface modified (negatively charged) silica, but no good correlation with the charge density was found when surfaces were modified by organic layers.
18. Vlassiouk, I.; Krasnoslobodtsev, A.; Smirnov, S. N.; Germann, M. Direct' Detection and Separation of DNA Using Nanoporous Alumina Filters. Langmui 2004, 20, 9913-9915.