A coculture assay to visualize and monitor interactions between migrating glioma cells and nerve fibers

Nature Protocols

Volumn 4, Issue 6, 2009, Pages 923-927

  Oellers, Patrick ^a   Schallenberg, Maurice ^b   Stupp, Tobias ^a   Charalambous, Petar ^a,c   Senner, Volker ^a   Paulus, Werner ^a   Thanos, Solon ^a,c  

^a UNIVERSITY HOSPITAL MÜNSTER   (Germany)

^b UNIVERSITY HOSPITAL ESSEN   (Germany)

^c Interdisciplinary Center of Clinical Research IZKF   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL INTERACTION; CELL MIGRATION; CELL TYPE; CHICKEN; COCULTURE; CONFOCAL MICROSCOPY; CONTROLLED STUDY; EMBRYO; EXTRACELLULAR MATRIX; GLIOMA CELL; HUMAN; HUMAN CELL; IN VITRO STUDY; MICROSCOPY; MYELINATED NERVE; NERVE FIBER; NONHUMAN; PRIORITY JOURNAL; RAT;

ANIMALS; AXONS; CELL COMMUNICATION; CELL LINE, TUMOR; CELL MOVEMENT; CHICK EMBRYO; COCULTURE TECHNIQUES; GLIOMA; HUMANS; MICROSCOPY, CONFOCAL; MICROSCOPY, VIDEO; MODELS, NEUROLOGICAL; NEOPLASM INVASIVENESS; NERVE FIBERS, MYELINATED; RETINA;

EID: 66749190031     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2009.62     Document Type: Article

References (25)

1. Ohgaki, H. & Kleihues, P. Genetic pathways to primary and secondary glioblastoma. Am. J. Pathol. 170, 1445-1453 (2007).
2. Stupp, R. et al. Radiotherapy plus concomitant and adjuvant temozolomide for glioblastoma. N. Engl. J. Med. 352, 987-996 (2005).
3. Giese, A., Bjerkvig, R., Berens, M.E. & Westphal, M. Cost of migration: invasion of malignant gliomas and implications for treatment. J. Clin. Oncol. 21, 1624-1636 (2003).
4. Boyden, S. The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes. J. Exp. Med. 115, 453-466 (1962).
5. Schichor, C. et al. The brain slice chamber, a novel variation of the Boyden Chamber Assay, allows time-dependent quantification of glioma invasion into mammalian brain in vitro. J. Neurooncol. 73, 9-18 (2005).
6. Valster, A. et al. Cell migration and invasion assays. Methods 37, 208-215 (2005).
7. Demuth, T. & Berens, M.E. Molecular mechanisms of glioma cell migration and invasion. J. Neurooncol. 70, 217-228 (2004).
8. Auer, R.N., Del Maestro, R.F. & Anderson, R. A simple and reproducible experimental in vivo glioma model. Can. J. Neurol. Sci. 8 325-331 (1981).
9. Cretu, A., Fotos, J.S., Little, B.W. & Galileo, D.S. Human and rat glioma growth, invasion, and vascularization in a novel chick embryo brain tumor model. Clin. Exp. Metastasis 22, 225-236 (2005).
10. Claes, A., Idema, A.J. & Wesseling, P. Diffuse glioma growth: A guerilla war. Acta Neuropathol. 114, 443-458 (2007).
11. Laerum, O.D. et al. Invasiveness in vitro and biological markers in human primary glioblastomas. J. Neurooncol. 54, 1-8 (2001).
12. Giese, A. et al. Migration of human glioma cells on myelin. Neurosurgery 38, 755-764 (1996).
13. Tatenhorst, L., Puttmann, S., Senner, V. & Paulus, W. Genes associated with fast glioma cell migration in vitro and in vivo. Brain Pathol. 15, 46-54 (2005).
14. Mariani, L. et al. Death-associated protein 3 (Dap-3) is overexpressed in invasive glioblastoma cells in vivo and in glioma cell lines with induced motility phenotype in vitro. Clin. Cancer Res. 7, 2480-2489 (2001).
15. Nakada, M. et al. Molecular targets of glioma invasion. Cell Mol. Life Sci. 64, 458-478 (2007).
16. Nakazawa, T., Tachi, S., Aikawa, E. & Ihnuma, M. Formation of the myelinated nerve fiber layer in the chicken retina. Glia 8, 114-121 (1993).
17. Ono, K., Tsumori, T., Kishi, T., Yokota, S. & Yasui, Y. Developmental appearance of oligodendrocytes in the embryonic chick retina. J. Comp. Neurol. 398, 309-322 (1998).
18. Mey, J. & Thanos, S. Ontogenetic changes in the regenerative ability of chick retinal ganglion cells as revealed by organ explants. Cell Tissue Res. 264, 347-355 (1991).
19. Oellers, P., Schroüer, U., Senner, V., Paulus, W. & Thanos, S. Rocks are expressed in brain tumors and are required for glioma cell migration on myelinated axons. Glia 57, 499-509 (2008).
20. Watkins, T.A., Emery, B., Malinyawe, S. & Barres, B.A. Distinct stages of myelination regulated by gamma-secretase and astrocytes in a rapidly myelinating coculture system. Neuron 60, 555-569 (2008).
21. Gage, F.H. Neurogenesis in the adult brain. J. Neurosci. 22, 612-613 (2002).
22. Karimi-Abdolrezaee, S., Eftekharpour, E., Wang, J., Morshead, C.M. & Fehlings, M.G. Delayed transplantation of adult neural precursor cells promotes remyelination and functional neurological recovery after spinal cord injury. J. Neurosci. 26, 3377-3389 (2006).
23. Meyer, J.S., Katz, M.L. & Kirk, M.D. Stemcells for retinal degenerative disorders. Ann. N Y Acad. Sci. 1049, 135-145 (2005).
24. Charalambous, P., Hurst, L. & Thanos, S. Engrafted chicken neural tube-derived stem cells support the innate propensity for axonal regeneration within the rat optic nerve. Invest. Ophthalmol. Vis. Sci. 49, 3513-3524 (2008).
25. Lee, J.E., Liang, K.J., Fariss, R.N. & Wong, W.T. Ex vivo dynamic imaging of retinal microglia using time-lapse confocal microscopy. Invest. Ophthalmol. Vis. Sci. 49, 4169-4176 (2008).