Multiparameter single molecule spectroscopy gives insight into the complex photophysics of fluorescence energytransfer (FRET) coupled biosystems

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Volumn 7190, Issue , 2009, Pages

  Schleifenbaum, F ^a   Blum, C ^a   Elgass, K ^a   Peter, S ^a   Subramaniam, V ^b   Meixner, A J ^a  

^a UNIVERSITY OF TÜBINGEN   (Germany)

^b UNIVERSITY OF TWENTE   (Netherlands)

Author keywords

Autofluorescent protein; DsRed; FRET; SERS; Single molecule

Indexed keywords

AUTOFLUORESCENT PROTEIN; DSRED; FRET; SERS; SINGLE MOLECULE;

BIOLOGICAL SYSTEMS; CHROMOPHORES; ENERGY TRANSFER; FLUORESCENCE; FLUORESCENCE MICROSCOPY; NANOPARTICLES; PROBES; SEMICONDUCTOR QUANTUM DOTS; SURFACES;

MOLECULES;

EID: 66749158121     PISSN: 16057422     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.809298     Document Type: Conference Paper

References (36)

1. W. E. Moerner, "New Directions in Single-Molecule Imaging and Analysis", Proc. Natl. Acad. Sci. USA 104, 12596 (2007). (Pubitemid 47255200)
2. F. Schleifenbaum, C. Blum, V. Subramaniam, and A. J. Meixner, "Single Molecule Spectral Dynamics at Room Temperature", Mol. Physics. in press (2008).
3. C. Blum, V. Subramaniam, F. Schleifenbaum, F. Stracke, B. Angres, A. Terskikh, and A. J. Meixner, "Single Molecule Fluorescence Spectroscopy of Mutants of the Discosoma Red Fluorescent Protein Dsred", Chemical Physics Letters 362, 355 (2002).
4. M. Cotlet, P. M. Goodwin, G. S. Waldo, and J. H. Werner, "A Comparison of the Fluorescence Dynamics of Single Molecules of a Green Fluorescent Protein: One-Versus Two-Photon Excitation", ChemPhysChem 7, 250 (2006).
5. R. Gronheid, J. Hofkens, F. Köhn, T. Weil, E. Reuther, K. Müllen, and F. C. d. Schryver, "Intramolecular Forster Energy Transfer in Dendritic Systems at the Single Molecule Level", J. Am. Chem. Soc. 124, 2418 (2002). (Pubitemid 34251739)
6. A. Otto, "What Is Observed in Single Molecule Sers, and Why?", J. Raman Spectrosc. 33, 593 (2002).
7. T. Vosgröne and A. J. Meixner, "Surface-and Resonance-Enhanced Micro-Raman Spectroscopy of Xanthene Dyes: From the Ensemble to Single Molecules", ChemPhysChem 6, 154 (2005). (Pubitemid 40203549)
8. M. Cotlet, J. Hofkens, S. Habuchi, G. Dirix, M. v. Guyse, J. Michiels, J. Vanderleyden, and F. C. d. Schryver, "Identification of Different Emitting Species in the Red Fluroescent Protein Dsred by Means of Ensemble and Single-Molecule Spectroscopy", Proc. Natl. Acad. Sci. USA 98, 14398 (2001). (Pubitemid 33121403)
9. C. Blum, Y. Cesa, M. Escalante, and V. Subramaniam, "Multimode Microscopy: Spectral and Lifetime Imaging ", J. R. Soc. Interface 6, 35 (2009).
10. C. A. M. S. Ralf Kühnemuth, "Principles of Single Molecule Multiparameter Fluorescence Spectroscopy", Single Molecules 2, 251 (2001).
11. D. Yarbrough, R. M. Wachter, K. Kallio, M. V. Matz, and S. J. Remington, "Refined Crystal Structure of Dsred, a Red Fluorescent Protein from Coral, at 2.0-a Resolution", Proc. Natl. Acad. Sci. USA 98, 462 (2001). (Pubitemid 32105064)
12. C. Blum, A. J. Meixner, and V. Subramaniam, "Single Oligomer Spectra Probe Chromophore Nanoenvironments of Tetrameric Fluorescent Proteins", J. Am. Chem. Soc. 128, 8664 (2006).
13. C. Blum, A. P. Mosk, I. S. Nikolaev, V. Subramaniam, and W. L. Vos, "Color Control of Natural Fluorescent Proteins by Photonic Crystals", Small 4, 492 (2008).
14. J. L. Tubbs, J. A. Tainer, and E. D. Getzoff, "Crystallographic Structures of Discosoma Red Fluorescent Protein with Immature and Mature Chromophores: Linking Peptide Bond Trans-Cis Isomerization and Acylimine Formation in Chromophore Maturation", Biochemistry 44, 9833 (2005). (Pubitemid 41033449)
15. G. Baird, D. Zacharias, and R. Tsien, "Biochemistry, Mutagenesis, and Oligomerization of Dsred, a Red Fluorescent Protein from Coral", Proc. Natl. Acad. Sci. USA 97, 119984 (2000).
16. V. V. Verkhusha, D. M. Chudakov, N. G. Gurskaya, S. Lukyanov, and K. A. Lukyanov, "Common Pathway for the Red Chromophore Formation in Fluorescent Proteins and Chromoproteins", Chemistry & Biology 11, 845 (2004). (Pubitemid 38295761)
17. M. F. Garcia-Parajo, M. Koopman, E. M. H. P. v. Dijk, V. Subramaniam, and N. F. v. Hulst, "The Nature of Fluorescence Emission in the Red Fluorescent Protein Dsred, Revealed by Single-Molecule Detection", Proc. Natl. Acad. Sci. USA 98, 14392 (2001). (Pubitemid 33121402)
18. C. Blum, A. J. Meixner, and V. Subramaniam, "Spectral Versatility Off Single Reef Coral Fluorescent Proteins Detected by Spectrally-Resolved Single Molecule Spectroscopy", ChemPhysChem 9, 310 (2008).
19. F. Schleifenbaum, K. Elgass, C. Blum, V. Subramaniam, and A. J. Meixner, "New Insights into the Photophysics of Dsred by Combined Spectral and Time Domain Spectroscopy on Single Proteins", J. Phys. Chem. B 112, 7669 (2008). Proc. of SPIE Vol. 7190 71900U-7
20. T. A. Schüttrigkeit, U. Zachariae, T. v. Feilitzsch, J. Wiehler, J. v. Hummel, B. Steipe, and M. E. Michel-Beyerle, "Picosecond Time-Resolved Fret in the Fluorescent Protein from Discosoma Red (Wt-Dsred)", ChemPhysChem 5, 325 (2001). (Pubitemid 33274858)
21. C. Blum and V. Subramaniam, "Single-Molecule Spectroscopy of Fluorescent Proteins", Anal. Bioanal. Chem. 393, 527 (2009). (Pubitemid 50301648)
22. B. Lounis, J. Deich, F. I. Rosell, S. G. Boxer, and W. E. Moerner, "Photophysics of Dsred, a Red Fluorescent Protein, from the Ensemble to the Single-Molecule Level", J. Phys. Chem. B 105, 5048 (2001). (Pubitemid 35372670)
23. L. W. Runnels and S. F. Scarlata, "Theory and Application of Fluorescence Homotransfer to Melittin Oligomerization", Biophysical Journal 69, 1569 (1995).
24. M. Cotlet, J. Hofkens, M. Maus, T. Gensch, M. Van der Auweraer, J. Michiels, G. Dirix, M. Van Guyse, J. Vanderleyden, A. J. W. G. Visser, and F. C. De Schryver, "Excited-State Dynamics in the Enhanced Green Fluorescent Protein Mutant Probed by Picosecond Time-Resolved Single Photon Counting Spectroscopy", J. Phys. Chem. B 105, 4999 (2001). (Pubitemid 35372666)
25. L. Cao, P. Diao, L. Tong, T. Zhu, and Z. Liu, "Surface-Enhanced Raman Scattering of P-Aminothiophenol on a Au(Core)/Cu(Shell) Nanoparticle Assembly", ChemPhysChem 6, 913 (2005).
26. J. A. Dieringer, R. B. Lettan, K. A. Scheidt, and R. P. Van Duyne, "A Frequency Domain Existence Proof of Single-Molecule Surface-Enhanced Raman Spectroscopy", Journal of the American Chemical Society 129, 16249 (2007).
27. S. G. Kruglik, V. Subramaniam, J. Greve, and C. Otto, "Resonance Cars Study of the Structure of "Green" and "Red" Chromophores within the Red Fluorescent Protein Dsred", J. Am. Chem. Soc. 124, 10992 (2002). (Pubitemid 35025654)
28. A. Otto, "Theory of First Layer and Single Molecule Surface Enhanced Raman Scattering (Sers)", Phys. Status Solidi A 188, 1455 (2001). (Pubitemid 33729145)
29. R. Petry, M. Schmitt, and J. Popp, "Raman Spectroscopy-a Prospective Tool in the Life Sciences", ChemPhysChem 4, 14 (2003).
30. P. Schellenberg, E. Johnson, A. P. Esposito, P. J. Reid, and W. W. Parson, "Resonance Raman Scattering by the Green Fluorescent Protein and an Analogue of Its Chromophore", J. Phys. Chem. B 105, 5316 (2001).
31. K. Kneipp, Y. Wang, H. Kneipp, L. T. Perelman, I. Itzkan, R. R. Dasari, and M. S. Feld, "Single Molecule Detection Using Surface-Enhanced Raman Scattering (Sers", Phys. Rev. Lett. 78, 1667-1670 (1997) 78, 1667 (1997). (Pubitemid 127648192)
32. S. Nie and S. R. Emory, "Probing Single Molecules and Single Nanoparticles by Surface-Enhanced Raman Scattering", Science 21, 1102 (1997). (Pubitemid 27097304)
33. S. Garoff, D. A. Weitz, T. J. Gramila, and C. D. Hanson, "Optical Absorption Resonances of Dye-Coated Silver-Island Films", Opt. Lett. 6, 245 (1981).
34. S. Habuchi, M. Cotlet, R. Gronheid, G. Dirix, J. Michiels, J. Vanderleyden, F. C. D. Schryver, and J. Hofkens, "Single-Molecule Surface Enhanced Resonance Raman Spectroscopy of the Enhanced Green Fluorescent Protein", J. Am. Chem. Soc. 125, 8446 (2003). (Pubitemid 36858408)
35. X. He, A. F. Bell, and P. J. Tonge, "Isotopic Labeling and Normal-Mode Analysis of a Model Green Fluorescent Protein Chromophore", J. Phys. Chem. B 106, 6056 (2002). (Pubitemid 35281853)
36. F. Schleifenbaum, S. Peter, and A. J. Meixner, "Detecting the Same Individual Molecule and Its Photoproducts Via Fluorescence and Surface Enhanced Raman Spectroscopic Imaging", (submitted).