Real-time quantitative PCR to determine chlamydial load in men and women in a community setting

Journal of Clinical Microbiology

Volumn 47, Issue 6, 2009, Pages 1824-1829

  Wiggins, R ^a,e   Graf, S ^b   Low, N ^c   Horner, P J ^d  

^a Department of Clinical Sciences South Bristol ^*  (United Kingdom)

^b FEDERAL OFFICE OF PUBLIC HEALTH   (Switzerland)

^c UNIVERSITY OF BERN   (Switzerland)

^d UNIVERSITY OF BRISTOL   (United Kingdom)

^e UNIVERSITY OF YORK   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ADOLESCENT; ADULT; AGE DISTRIBUTION; ARTICLE; BACTERIAL COUNT; CHLAMYDIA TRACHOMATIS; COMMUNITY CARE; FEMALE; HELP SEEKING BEHAVIOR; HUMAN; MAJOR CLINICAL STUDY; MALE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SCREENING TEST; SEX DIFFERENCE; URINALYSIS; VAGINA;

ADOLESCENT; ADULT; CHLAMYDIA INFECTIONS; CHLAMYDIA TRACHOMATIS; COMMUNITY-ACQUIRED INFECTIONS; FEMALE; HUMANS; MALE; POLYMERASE CHAIN REACTION; SELF-EXAMINATION; URINE; VAGINA; VULVA; YOUNG ADULT;

CHLAMYDIA; CHLAMYDIA TRACHOMATIS;

EID: 66749150512     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.00005-09     Document Type: Article

References (11)

1. Eckert, L. O., R. J. Suchland, S. E. Hawes, and W. E. Stamm. 2000. Quantitative Chlamydia trachomatis cultures: correlation of chlamydial inclusion-forming units with serovar, age, sex, and race. J. Infect. Dis. 182:540-544.
2. Geisler, W. M., R. J. Suchland, W. L. Whittington, and W. E. Stamm. 2001. Quantitative culture of Chlamydia trachomatis: relationship of inclusionforming units produced in culture to clinical manifestations and acute inflammation in urogenital disease. J. Infect. Dis. 184:1350-1354.
3. Geisler, W. M., C. Wang, S. G. Morrison, C. M. Black, C. I. Bandea, and E. W. Hook. 2008. The natural history of untreated Chlamydia trachomatis infection in the interval between screening and returning for treatment. Sex. Transm. Dis. 35:119-123.
4. Herring, A., D. Longhurst, K. Eastick, I. Paul, J. Leece, O. Caul, and P. Horner. 2002. Which is the best specimen for chlamydial screening? Chlamydial 'loads' in urine and vulvo/vaginal swabs, p. 421-424. In J. Schachter, G. Christiansen, I. N. Clarke, M. R. Hammerschlag, B. Kaltenboeck, C. C. Kuo, R. Rank, G. Ridgway, P. Saikku, W. E. Stamm, R. S. Stephens, J. T. Summersgill, P. Timms, and P. B. Wyrick (ed.), Proceedings of the Tenth International Symposium on Human Chlamydial Infections. International Chlamydia Symposium, San Francisco, CA.
5. Horner, P., S. Skidmore, A. Herring, J. Sell, I. Paul, O. Caul, M. Egger, A. McCarthy, E. Sanford, C. Salisbury, J. MacLeod, J. Sterne, N. Low, et al. 2005. Enhanced enzyme immunoassay with negative-gray-zone testing compared to a single nucleic acid amplification technique for community-based chlamydial screening of men. J. Clin. Microbiol. 43:2065-2069.
6. Horner, P., and F. Boag. 2006. UK national guideline for the management of genital tract infection with Chlamydia trachomatis. BASHH, London, United Kingdom. http://www.bashh.org/documents/61/61.pdf.
7. Low, N., A. McCarthy, J. Macleod, C. Salisbury, R. Campbell, T. E. Roberts, P. Horner, S. Skidmore, J. A. Sterne, E. Sanford, F. Ibrahim, A. Holloway, R. Patel, P. M. Barton, S. M. Robinson, N. Mills, A. Graham, A. Herring, E. O. Caul, G. Davey Smith, F. D. Hobbs, J. D. Ross, M. Egger, et al. 2007. Epidemiological, social, diagnostic and economic evaluation of population screening for genital chlamydial infection. Health Technol. Assess. (Winchester, England) 11:1-184.
8. Michel, C. E., C. Sonnex, C. A. Carne, J. A. White, J. P. Magbanua, E. C. Nadala, and H. H. Lee. 2007. Chlamydia trachomatis load at matched anatomic sites: implications for screening strategies. J. Clin. Microbiol. 45:1395-1402.
9. Qiagen. 2008. C. trachomatis PCR kit handbook. Qiagen, Hilden, Germany. http://www1.qiagen.com/literature/handbooks/literature.aspx?id=1000839.
10. Rogers, S. M., W. C. Miller, C. F. Turner, J. Ellen, J. Zenilman, R. Rothman, M. A. Villarroel, A. Al-Tayyib, P. Leone, C. Gaydos, L. Ganapathi, M. Hobbs, and D. Kanouse. 2008. Concordance of Chlamydia trachomatis infections within sexual partnerships. Sex. Transm. Infect. 84:23-28.
11. Skidmore, S., P. Horner, A. Herring, J. Sell, I. Paul, J. Thomas, E. O. Caul, M. Egger, A. McCarthy, E. Sanford, C. Salisbury, J. MacLeod, J. A. C. Sterne, N. Low, et al. 2006. Vulvovaginal-swab or first-catch urine specimen to detect Chlamydia trachomatis in women in a community setting? J. Clin. Microbiol. 44:4389-4394.