Development and characterization of a genotype 4 hepatitis E virus cell culture system using a HE-JF5/15F strain recovered from a fulminant hepatitis patient

Journal of Clinical Microbiology

Volumn 47, Issue 6, 2009, Pages 1906-1910

  Tanaka, Toshinori ^a   Takahashi, Masaharu ^a   Takahashi, Hideyuki ^a   Ichiyama, Koji ^a   Hoshino, Yu ^a   Nagashima, Shigeo ^a   Mizuo, Hitoshi ^b   Okamoto, Hiroaki ^a  

^a JICHI MEDICAL UNIVERSITY   (Japan)

^b Kin ikyo Chuo Hospital   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; VIRUS RNA;

ADULT; AMINO ACID SEQUENCE; ARTICLE; CASE REPORT; GENE MUTATION; GENOTYPE; HEPATITIS E; HEPATITIS E VIRUS; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; MALE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; VIROGENESIS; VIRUS CHARACTERIZATION; VIRUS CULTURE; VIRUS GENE; VIRUS LOAD; VIRUS STRAIN;

AMINO ACID SUBSTITUTION; CELL CULTURE TECHNIQUES; CELL LINE; GENOTYPE; HEPATITIS E; HEPATITIS E VIRUS; HUMANS; MALE; MIDDLE AGED; MOLECULAR SEQUENCE DATA; MUTATION, MISSENSE; RNA, VIRAL; SEQUENCE ANALYSIS, DNA;

HEPATITIS E VIRUS;

EID: 66749127881     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.00629-09     Document Type: Article

References (43)

1. Agrawal, S., D. Gupta, and S. K. Panda. 2001. The 3′ end of hepatitis E virus (HEV) genome binds specifically to the viral RNA-dependent RNA polymerase (RdRp). Virology 282:87-101.
2. Dalton, H. R., R. Bendall, S. Ijaz, and M. Banks. 2008. Hepatitis E: an emerging infection in developed countries. Lancet Infect. Dis. 8:698-709.
3. Emerson, S. U., D. Anderson, A. Arankalle, X. J. Meng, M. Purdy, G. G. Schlauder, and S. A. Tsarev. 2004. Hepevirus, p. 853-855. In C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger, and L. A. Ball (ed.), Virus taxonomy: VIIIth report of the ICTV. Elsevier/Academic Press, London, United Kingdom.
4. Emerson, S. U., and R. H. Purcell. 2006. Hepatitis E virus, p. 3047-3058. In D. M. Knipe, P. M. Howley, D. E. Griffin, R. A. Lamb, M. A. Martin, B. Roizman, and S. E. Straus (ed.), Fields virology, 5th ed. Lippincott Williams & Wilkins, Philadelphia, PA.
5. Graff, J., A. Normann, S. M. Feinstone, and B. Flehmig. 1994. Nucleotide sequence of wild-type hepatitis A virus GBM in comparison with two cell culture-adapted variants. J. Virol. 68:548-554. (Pubitemid 24974664)
6. Graff, J., H. Nguyen, C. Kasorndorkbua, P. G. Halbur, M. St. Claire, R. H. Purcell, and S. U. Emerson. 2005. In vitro and in vivo mutational analysis of the 3′-terminal regions of hepatitis E virus genomes and replicons. J. Virol. 79:1017-1026.
7. Graff, J., U. Torian, H. Nguyen, and S. U. Emerson. 2006. A bicistronic subgenomic mRNA encodes both the ORF2 and ORF3 proteins of hepatitis E virus. J. Virol. 80:5919-5926. (Pubitemid 43849169)
8. Harrison, T. J. 1999. Hepatitis E virus - an update. Liver 19:171-176. (Pubitemid 29261581)
9. Hu, N. Z., Y. Z. Hu, H. J. Shi, G. D. Liu, and S. Qu. 2002. Mutational characteristics in consecutive passage of rapidly replicating variants of hepatitis A virus strain H2 during cell culture adaptation. World J. Gastroenterol. 8:872-878.
10. Huang, Y. W., T. Opriessnig, P. G. Halbur, and X. J. Meng. 2007. Initiation at the third in-frame AUG codon of open reading frame 3 of the hepatitis E virus is essential for viral infectivity in vivo. J. Virol. 81:3018-3026. (Pubitemid 46447067)
11. Ina, Y. 1994. ODEN: a program package for molecular evolutionary analysis and database search of DNA and amino acid sequences. Comput. Appl. Biosci. 10:11-12.
12. Inoue, J., T. Nishizawa, M. Takahashi, T. Aikawa, H. Mizuo, K. Suzuki, T. Shimosegawa, and H. Okamoto. 2006. Analysis of the full-length genome of genotype 4 hepatitis E virus isolates from patients with fulminant or acute self-limited hepatitis E. J. Med. Virol. 78:476-484.
13. Jansen, R. W., J. E. Newbold, and S. M. Lemon. 1988. Complete nucleotide sequence of a cell culture-adapted variant of hepatitis A virus: comparison with wild-type virus with restricted capacity for in vitro replication. Virology 163:299-307. (Pubitemid 18115455)
14. Khuroo, M. S., and S. Kamili. 2003. Aetiology, clinical course and outcome of sporadic acute viral hepatitis in pregnancy. J. Viral Hepat. 10:61-69. (Pubitemid 36237814)
15. Kosaka, Y., K. Takase, M. Kojima, M. Shimizu, K. Inoue, M. Yoshiba, S. Tanaka, Y. Akahane, H. Okamoto, F. Tsuda, Y. Miyakawa, and M. Mayumi. 1991. Fulminant hepatitis B: induction by hepatitis B virus mutants defective in the precore region and incapable of encoding e antigen. Gastroenterology 100:1087-1094.
16. Liang, T. J., K. Hasegawa, N. Rimon, J. R. Wands, and E. Ben-Porath. 1991. A hepatitis B virus mutant associated with an epidemic of fulminant hepatitis. N. Engl. J. Med. 324:1705-1709.
17. Lorenzo, F. R., T. Tanaka, H. Takahashi, K. Ichiyama, Y. Hoshino, K. Yamada, J. Inoue, M. Takahashi, and H. Okamoto. 2008. Mutational events during the primary propagation and consecutive passages of hepatitis E virus strain JE03-1760F in cell culture. Virus Res. 137:86-96.
18. Lu, L., C. Li, and C. H. Hagedorn. 2006. Phylogenetic analysis of global hepatitis E virus sequences: genetic diversity, subtypes and zoonosis. Rev. Med. Virol. 16:5-36.
19. Meng, X.-J., R. H. Purcell, P. G. Haubur, J. R. Lehman, D. M. Webb, T. S. Tsareva, J. S. Haynes, B. J. Thacker, and S. U. Emerson. 1997. A novel virus in swine is closely related to the human hepatitis E virus. Proc. Natl. Acad. Sci. USA 94:9860-9865.
20. Meng, X.-J., P. G. Halbur, M. S. Shapiro, S. Govindarajan, J. D. Bruna, I. K. Mushahwar, R. H. Purcell, and S. U. Emerson. 1998. Genetic and experimental evidence for cross-species infection by swine hepatitis E virus. J. Virol. 72:9714-9721. (Pubitemid 28520835)
21. Meng, X. J. 2005. Hepatitis E as a zoonotic disease, p. 611-623. In H. C. Thomas, S. Lemon, and A. J. Zuckerman (ed.), Viral hepatitis, 3rd ed. Blackwell Publishing, Malden, MA.
22. Mizuo, H., Y. Yazaki, K. Sugawara, F. Tsuda, M. Takahashi, T. Nishizawa, and H. Okamoto. 2005. Possible risk factors for the transmission of hepatitis E virus and for the severe form of hepatitis E acquired locally in Hokkaido, Japan. J. Med. Virol. 76:341-349.
23. Navaneethan, U., M. Al Mohajer, and M. T. Shata. 2008. Hepatitis E and pregnancy: understanding the pathogenesis. Liver Int. 28:1190-1199.
24. Okamoto, H., M. Takahashi, T. Nishizawa, K. Fukai, U. Muramatsu, and A. Yoshikawa. 2001. Analysis of the complete genome of indigenous swine hepatitis E virus isolated in Japan. Biochem. Biophys. Res. Commun. 289:929-936.
25. Okamoto, H., M. Takahashi, and T. Nishizawa. 2003. Features of hepatitis E virus infection in Japan. Intern. Med. 42:1065-1071.
26. Okamoto, H. 2007. Genetic variability and evolution of hepatitis E virus. Virus Res. 127:216-228. (Pubitemid 47058196)
27. Provost, P. J., and M. R. Hilleman. 1979. Propagation of human hepatitis A virus in cell culture in vitro. Proc. Soc. Exp. Biol. Med. 160:213-221.
28. Purcell, R. H., and S. U. Emerson. 2008. Hepatitis E: an emerging awareness of an old disease. J. Hepatol. 48:494-503.
29. Sato, S., K. Suzuki, Y. Akahane, K. Akamatsu, K. Akiyama, K. Yunomura, F. Tsuda, T. Tanaka, H. Okamoto, Y. Miyakawa, and M. Mayumi. 1995. Hepatitis B virus strains with mutations in the core promoter in patients with fulminant hepatitis. Ann. Intern. Med. 122:241-248.
30. Schlauder, G. G., and I. K. Mushahwar. 2001. Genetic heterogeneity of hepatitis E virus. J. Med. Virol. 65:282-292.
31. Smith, J. L. 2001. A review of hepatitis E virus. J. Food Prot. 64:572-586. (Pubitemid 32368287)
32. Suzuki, K., T. Aikawa, and H. Okamoto. 2002. Fulminant hepatitis E in Japan. N. Engl. J. Med. 347:1456.
33. Takahashi, K., N. Kitajima, N. Abe, and S. Mishiro. 2004. Complete or near-complete nucleotide sequences of hepatitis E virus genome recovered from a wild boar, a deer, and four patients who ate the deer. Virology 330:501-505. (Pubitemid 39572856)
34. Takahashi, M., T. Nishizawa, H. Miyajima, Y. Gotanda, T. Iita, F. Tsuda, and H. Okamoto. 2003. Swine hepatitis E virus strains in Japan form four phylogenetic clusters comparable with those of Japanese isolates of human hepatitis E virus. J. Gen. Virol. 84:851-862.
35. Takahashi, M., T. Tanaka, M. Azuma, E. Kusano, T. Aikawa, T. Shibayama, Y. Yazaki, H. Mizuo, J. Inoue, and H. Okamoto. 2007. Prolonged fecal shedding of hepatitis E virus (HEV) during sporadic acute hepatitis E: evaluation of infectivity of HEV in fecal specimens in a cell culture system. J. Clin. Microbiol. 45:3671-3679.
36. Takahashi, M., Y. Hoshino, T. Tanaka, H. Takahashi, T. Nishizawa, and H. Okamoto. 2008. Production of monoclonal antibodies against hepatitis E virus capsid protein and evaluation of their neutralizing activity in a cell culture system. Arch. Virol. 153:657-666.
37. Tam, A. W., M. M. Smith, M. E. Guerra, C. Huang, D. W. Bradley, K. E. Fry, and G. R. Reyes. 1991. Hepatitis E virus (HEV): molecular cloning and sequence of the full-length viral genome. Virology 185:120-130.
38. Tanaka, T., M. Takahashi, E. Kusano, and H. Okamoto. 2007. Development and evaluation of an efficient cell-culture system for Hepatitis E virus. J. Gen. Virol. 88:903-911. (Pubitemid 47281165)
39. Tei, S., N. Kitajima, K. Takahashi, and S. Mishiro. 2003. Zoonotic transmission of hepatitis E virus from deer to human beings. Lancet 362:371-373. (Pubitemid 36945060)
40. Tyagi, S., M. Surjit, A. K. Roy, S. Jameel, and S. K. Lal. 2004. The ORF3 protein of hepatitis E virus interacts with liver-specific alpha1-microglobulin and its precursor alpha1-microglobulin/bikunin precursor (AMBP) and expedites their export from the hepatocyte. J. Biol. Chem. 279:29308-29319.
41. Wang, Y., H. Zhang, R. Ling, H. Li, and T. J. Harrison. 2000. The complete sequence of hepatitis E virus genotype 4 reveals an alternative strategy for translation of open reading frames 2 and 3. J. Gen. Virol. 81:1675-1686.
42. Yamada, K., M, Takahashi, Y. Hoshino, H. Takahashi, K. Ichiyama, T. Tanaka, and H. Okamoto. 2009. Construction of an infectious cDNA clone of hepatitis E virus strain JE03-1760F that can propagate efficiently in cultured cells. J. Gen. Virol. 90:457-462.
43. Yazaki, Y., H. Mizuo, M. Takahashi, T. Nishizawa, N. Sasaki, Y. Gotanda, and H. Okamoto. 2003. Sporadic acute or fulminant hepatitis E in Hokkaido, Japan, may be food-borne, as suggested by the presence of hepatitis E virus in pig liver as food. J. Gen. Virol. 84:2351-2357.