Cloning the structure genes and expression the N gene of porcine epidemic diarrhea virus DX

Virologica Sinica

Volumn 24, Issue 3, 2009, Pages 179-186

  Li, Jian Qiang ^a,b   Liu, Ji Xing ^b   Lan, Xi ^b   Cheng, Jie ^a   Wu, Run ^a   Lou, Zhong Zi ^b   Yin, Xiang Ping ^b   Li, Xue Rui ^b   Li, Bao Yu ^b   Yang, Bin ^b   Li, Zhi Yong ^b  

^a GANSU AGRICULTURAL UNIVERSITY   (China)

^b INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

Cloning; Expression; N gene; PEDV; Structure genes

Indexed keywords

ASPARAGINE; MEMBRANE PROTEIN; NUCLEOCAPSID PROTEIN; RESTRICTION ENDONUCLEASE; RESTRICTION ENZYME NCO I; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE; UNCLASSIFIED DRUG; VIRUS SPIKE PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHINA; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PHYLOGENY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PORCINE EPIDEMIC DIARRHEA VIRUS; PORCINE EPIDEMIC DIARRHEA VIRUS DX; PROTEIN FUNCTION; PROTEIN GLYCOSYLATION; RECOMBINANT PLASMID; SEQUENCE ANALYSIS; STRUCTURAL HOMOLOGY; TRANSCRIPTION REGULATION; VIRUS ENVELOPE; VIRUS GENE; VIRUS ISOLATION; VIRUS NUCLEOCAPSID; VIRUS SPIKE; VIRUS STRAIN; WESTERN BLOTTING;

PORCINE EPIDEMIC DIARRHEA VIRUS;

EID: 66649118029     PISSN: 16740769     EISSN: 1995820X     Source Type: Journal    
DOI: 10.1007/s12250-009-2982-y     Document Type: Article

References (20)

1. Bos E C, Luytjes W, vander Meulen H V, et al. 1996. The production of recombinant infectious DI-particles of a murine coronavirus in the absence of helper virus. Virology. 218: 52-60.
2. Chen J F, Feng L, Shi H Y, et al. 2007. Genetic variation and prolaryotic expression of N protein gene of porcine epidemic diarrhea virus CH/S strain. Chin J Prev Vet Med, 29: 856-860. (in Chinese)
3. Cheng Q H, Niu X Y. 1992. Investigation on the porcine epidemic diarrhea prevalent on Qinhai, Chin Qinhai J Anim Vet Sci, 22: 22-23. (in Chinese)
4. DeBouck P, Callebaut P, Pensaert M. 1982. Prevalence of the porcine epidemic diarrhea (PED) virus in the pig population of different countries, Proc Int Congr Pig Vet Soc, 7: 53.
5. Duarte M, Laude H. 1994. Sequence of the spike protein of the Porcine epidemic diarrhea virus. J Gen Virol, 75:1195-1200.
6. Egberink H F, Ederveen J, Callebaut P. 1988. Characterization of the structural proteins of porcine epidemic diarrhea virus, strain CV777. Am J Vet Res, 49: 1320-1324.
7. Fan J H, Li Y J. 2005. Cloning and sequence analysis of the M gene of Porcine epidemic diarrhea virus LJB/03. Virus Genes. 30: 69-73.
8. Ge J W, Li B X, Tang L J, et al. 2006. Cloning and sequence analysis of the N gene of porcine epidemic diarrhea virus LJB/03. Virus Genes 33: 215-219.
9. Hofmann M, Wyler R. 1987, Serologic study of the occurrence of epizootic viral diarrhea in swine in Switzerland, Schweiz Arch Tierheilkd, 129: 437.
10. Kang T J, Seo J E, Kim D H, et al. 2005. Cloning and sequence analysis of the Korean strain of spike gene of Porcine epidemic diarrhea virus and expression of its neutralizing epitope in plants. Protein Expression and Purification. 41: 378-383.
11. Lee H G, Yeo S H. 2003. Cloning and Sequence Analysis of the Nucleocapsid Gene of Porcine Epidemic Diarrhea Virus Chinju99. Virus Genes. 26: 207-212.
12. Master P S. 2006. The molecular biology of coronaviruses. Adv Virus Res, 66: 193-198
13. Mostl K, Horvath E, Burki F. 1990. Erhebungen uber porcine coronaviren in osterreich II porcine epidemic diarrhea virus (PEDV) der schweine. Wien Tierarztl Monatsschr. 77: 10-18.
14. Penzes Z, Gonzalez J M, Calvo E, et al. 2001. Complete Genome Sequence of Transmissible Gastroenteritis Coronavirus PUR46-MAD Clone and Evolution of the Purdue Virus Cluster. Virus Genes, 23: 105-118.
15. Shibata I, Tsuda T, Mori M. 2000. Isolation of porcine epidemic diarrhea virus in porcine cell cultures and experimental infection of pigs of different ages. Vet Microbiol, 72: 1732-1821
16. Singh M. 1999. A novel internal open reading frame product expressed from a polycistronic of porcine edpidemic diarrhea virus may not contribute to virus attenuation. J Gen Virol, 80: 1959-1963.
17. Straw B E, Allaire S D, Mengeling W L, et al. 2000. In: Disease of Swine (Translated by Zhao D M, Zhang Z Q, Shen J Z). 8th ed. Beijing: The Chinese Agriculture University Press, p181-187.
18. Tzipori S, Spradbrow P B. 1978. A cell culture vaccine against bovine ephemeral fever. Aust Vet J Jul. 54: 323-328.
19. Yeo S G, Hernandez M, Krell P J. 2003. Cloning and sequence analysis of the spike gene of Porcine epidemic diarrhea virus Chiju99. Virus Genes. 26: 239-246.
20. Wang Y, Zhang X. 1999. The nucleocapsid protein of coronavirus mouse hepatitis virus interacts with the cellular heterogeneous nuclear ribonucleoprotein A1 in vitro and in vivo. Virology 265: 96-109.