Effect of proline substitutions on stability and kinetic properties of a cold adapted subtilase

Journal of Biochemistry

Volumn 145, Issue 3, 2009, Pages 325-329

  Arnórsdóttir, Jóhanna ^a   Sigtryggsdóttir, Ásta Rós ^a   Thorbjarnardóttir, Sigríour H ^a   Kristjánsson, Magnús M ^a  

^a UNIVERSITY OF ICELAND   (Iceland)

Author keywords

Kinetic properties; Proline; Psychrophilic; Site directed mutagenesis; Stability

Indexed keywords

AQUALYSIN I; BACTERIAL PROTEIN; PROLINE; UNCLASSIFIED DRUG; MUTANT PROTEIN; RECOMBINANT PROTEIN; SERINE PROTEINASE; SUBTILISIN;

AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CATALYSIS; NONHUMAN; PROTEIN STABILITY; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY; THERMUS AQUATICUS; VIBRIO; AMINO ACID SEQUENCE; CHEMISTRY; COLD; ENZYME STABILITY; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; MOLECULAR GENETICS; PROTEIN SECONDARY STRUCTURE; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS;

THERMUS AQUATICUS; VIBRIO;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; COLD TEMPERATURE; ENZYME STABILITY; KINETICS; MOLECULAR SEQUENCE DATA; MUTANT PROTEINS; PROLINE; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, PROTEIN; SERINE ENDOPEPTIDASES; SUBTILISINS; VIBRIO;

EID: 66549096024     PISSN: 0021924X     EISSN: 17562651     Source Type: Journal    
DOI: 10.1093/jb/mvn168     Document Type: Article

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