Evaluation of six nucleic acid amplification tests used for diagnosis of Neisseria gonorrhoeae in Russia compared with an international strictly validated real-time porA pseudogene polymerase chain reaction

Journal of the European Academy of Dermatology and Venereology

Volumn 23, Issue 11, 2009, Pages 1246-1253

  Shipitsyna, E ^a   Zolotoverkhaya, E ^a   Hjelmevoll, So ^b   Maximova, A ^c   Savicheva, A ^a   Sokolovsky, E ^c   Skogen, V ^b   Domeika, M ^d   Unemo, M ^e  

^a D O OTT RESEARCH INSTITUTE OF OBSTETRICS   (Russian Federation)

^b UNIVERSITY HOSPITAL OF NORTH NORWAY   (Norway)

^c PAVLOV FIRST SAINT PETERSBURG STATE MEDICAL UNIVERSITY   (Russian Federation)

^d UPPSALA UNIVERSITY   (Sweden)

^e ÖREBRO UNIVERSITY HOSPITAL   (Sweden)

Author keywords

Neisseria gonorrhoeae; Nucleic acid amplification tests; PorA pseudogene PCR; Russia; Sensitivity and specificity

Indexed keywords

PORIN A;

ADOLESCENT; ADULT; ARTICLE; FEMALE; GONORRHEA; HUMAN; MAJOR CLINICAL STUDY; MALE; NEISSERIA GONORRHOEAE; NUCLEIC ACID AMPLIFICATION; NUCLEIC ACID SEQUENCE BASED AMPLIFICATION; PHARYNX; PREVALENCE; PRIORITY JOURNAL; PSEUDOGENE; REAL TIME POLYMERASE CHAIN REACTION; RECTUM; RUSSIAN FEDERATION; SENSITIVITY AND SPECIFICITY; URETHRA; URINE; UROGENITAL SCINTISCANNING; UTERINE CERVIX;

EID: 66349124670     PISSN: 09269959     EISSN: 14683083     Source Type: Journal    
DOI: 10.1111/j.1468-3083.2009.03290.x     Document Type: Article

References (30)

1. Whiley DM, Tapsall JW, Sloots TP. Nucleic acid amplification testing for Neisseria gonorrhoeae: an ongoing challenge. J Mol Diagn 2006 8 : 3 15.
2. Whiley DM, Garland SM, Harnett G et al. Exploring 'best practice' for nucleic acid detection of Neisseria gonorrhoeae. Sex Health 2008 5 : 17 23.
3. Savicheva A, Sokolovsky E, Frigo N et al. Guidelines for laboratory diagnosis of Neisseria gonorrhoeae in East-European countries. Part 2. Culture, non-culture methods, determination of antibiotic resistance, and quality assurance. Acta Medica Lituanica 2007 14 : 123 134.
4. Shipitsyna E, Guschin A, Maximova A et al. Comparison of microscopy, culture and in-house PCR and NASBA assays for diagnosis of Neisseria gonorrhoeae in Russia. APMIS 2008 116 : 133 138.
5. Unemo M, Savicheva A, Budilovskaya O et al. Laboratory diagnosis of Neisseria gonorrhoeae in St. Petersburg, Russia: inventory, performance characteristics and recommended optimizations. Sex Transm Infect 2006 82 : 41 44.
6. Unemo M, Vorobieva V, Firsova N et al. Neisseria gonorrhoeae population in Arkhangelsk, Russia: phenotypic and genotypic heterogeneity. Clin Microbiol Infect 2007 13 : 873 878. (Pubitemid 47244136)
7. Chaudhry U, Saluja D. Detection of Neisseria gonorrhoeae by PCR using orf1 gene as target. Sex Transm Infect 2002 78 : 72.
8. Goire N, Nissen MD, LeCornec GM et al. A duplex Neisseria gonorrhoeae real-time polymerase chain reaction assay targeting the gonococcal porA pseudogene and multicopy opa genes. Diagn Microbiol Infect Dis 2008 61 : 6 12.
9. Hjelmevoll SO, Olsen ME, Sollid JU et al. A fast real-time polymerase chain reaction method for sensitive and specific detection of the Neisseria gonorrhoeae porA pseudogene. J Mol Diagn 2006 8 : 574 581.
10. Tabrizi SN, Chen S, Tapsall J et al. Evaluation of opa-based real-time PCR for detection of Neisseria gonorrhoeae. Sex Transm Dis 2005 32 : 199 202.
11. Whiley DM, Buda PJ, Bayliss J et al. A new confirmatory Neisseria gonorrhoeae real-time PCR assay targeting the porA pseudogene. Eur J Clin Microbiol Infect Dis 2004 23 : 705 710.
12. Hjelmevoll SO, Olsen ME, Sollid JU et al. Clinical validation of a real-time polymerase chain reaction detection of Neisseria gonorrhoeae porA pseudogene versus culture technique. Sex Transm Dis 2008 35 : 517 520.
13. Shipitsyna E, Zolotoverkhaya E, Agné-Stadling I et al. First evaluation of six nucleic acid amplification tests (NAATs) widely used in the diagnosis of Chlamydia trachomatis in Russia. J Eur Acad Dermatol Venereol 2009 23 : 268 276.
14. Farrell DJ. Evaluation of AMPLICOR Neisseria gonorrhoeae PCR using cppB nested PCR and 16S rRNA PCR. J Clin Microbiol 1999 37 : 386 390.
15. Koumans EH, Black CM, Markowitz LE et al. Comparison of methods for detection of Chlamydia trachomatis and Neisseria gonorrhoeae using commercially available nucleic acid amplification tests and a liquid pap smear medium. J Clin Microbiol 2003 41 : 1507 1511.
16. Palmer HM, Mallinson H, Wood RL et al. Evaluation of the specificities of five DNA amplification methods for the detection of Neisseria gonorrhoeae. J Clin Microbiol 2003 41 : 835 837.
17. Smith DW, Tapsall JW, Lum G. Guidelines for the use and interpretation of nucleic acid detection tests for Neisseria gonorrhoeae in Australia: a position paper on behalf of the Public Health Laboratory Network. Commun Dis Intell 2005 29 : 358 365.
18. Tabrizi SN, Chen S, Cohenford MA et al. Evaluation of real time polymerase chain reaction assays for confirmation of Neisseria gonorrhoeae in clinical samples tested positive in the Roche Cobas Amplicor assay. Sex Transm Infect 2004 80 : 68 71.
19. Tapsall JW, Limnios EA, Nguyen NL et al. Cryptic-plasmid-free gonococci may contribute to failure of cppB gene-based assays to confirm results of BD ProbeTEC PCR for identification of Neisseria gonorrhoeae. J Clin Microbiol 2005 43 : 2036 2037.
20. Mahony JB, Luinstra KE, Tyndall M et al. Multiplex PCR for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in genitourinary specimens. J Clin Microbiol 1995 33 : 3049 3053.
21. Cook RL, Hutchison SL, Østergaard L et al. Systematic review: noninvasive testing for Chlamydia trachomatis and Neisseria gonorrhoeae. Ann Intern Med 2005 142 : 914 925.
22. Unemo M, Norlén O, Fredlund H. The porA pseudogene of Neisseria gonorrhoeae - low level of genetic polymorphism and a few, mainly identical, inactivating mutations. APMIS 2005 113 : 410 419.
23. Whiley DM, Anderson TP, Barrat K et al. Evidence that the gonococcal porA pseudogene is present in a broad range of Neisseria gonorrhoeae strains; suitability as a diagnostic target. Pathology 2006 38 : 445 448.
24. Schachter J, Moncada J, Liska S et al. Nucleic acid amplification tests in the diagnosis of chlamydial and gonococcal infections of the oropharynx and rectum in men who have sex with men. Sex Transm Dis 2008 35 : 637 642.
25. Ripa T, Nilsson P. A variant of Chlamydia trachomatis with deletion in cryptic plasmid: implications for use of PCR diagnostic tests. Euro Surveill 2006 11 : E061109. 2.
26. Chernesky MA, Martin DH, Hook EW 3rd et al. Ability of new APTIMA CT and APTIMA GC assays to detect Chlamydia trachomatis and Neisseria gonorrhoeae in male urine and urethral swabs. J Clin Microbiol 2005 43 : 127 131.
27. Lowe P, O'Loughlin P, Evans K et al. Comparison of the Gen-Probe APTIMA Combo 2 assay to the AMPLICOR CT/NG assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine samples from Australian men and women. J Clin Microbiol 2006 44 : 2619 2621.
28. Van Der Pol B, Ferrero DV, Buck-Barrington L et al. Multicenter evaluation of the BDProbeTec ET system for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine specimens, female endocervical swabs, and male urethral swabs. J Clin Microbiol 2001 39 : 1008 1016.
29. Moncada J, Schachter J, Hook EW 3rd et al. The effect of urine testing in evaluations of the sensitivity of the Gen-Probe Aptima Combo 2 assay on endocervical swabs for Chlamydia trachomatis and Neisseria gonorrhoeae: the infected patient standard reduces sensitivity of single site evaluation. Sex Transm Dis 2004 31 : 273 277.
30. Lindan C, Mathur M, Kumta S et al. Utility of pooled urine specimens for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in men attending public sexually transmitted infection clinics in Mumbai, India, by PCR. J Clin Microbiol 2005 43 : 1674 1677.