Repression of the acid ZrfA/ZrfB zinc-uptake system of Aspergillus fumigatus mediated by PacC under neutral, zinc-limiting conditions

International Microbiology

Volumn 12, Issue 1, 2009, Pages 39-47

  Amich, Jorge ^a   Leal, Fernando ^a   Calera, José A ^a  

^a UNIVERSITY OF SALAMANCA   (Spain)

Author keywords

Aspergillus fumigatus; PacC; Zinc; Zinc uptake system

Indexed keywords

ASPERGILLUS; ASPERGILLUS FUMIGATUS;

CATION TRANSPORT PROTEIN; FUNGAL PROTEIN; PACC PROTEIN, ASPERGILLUS; TRANSCRIPTION FACTOR; ZINC;

AMINO ACID SEQUENCE; ARTICLE; ASPERGILLUS FUMIGATUS; BIOSYNTHESIS; FUNGAL GENE; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; GENETICS; GROWTH, DEVELOPMENT AND AGING; ION TRANSPORT; METABOLISM; MOLECULAR GENETICS; MUTATION;

AMINO ACID SEQUENCE; ASPERGILLUS FUMIGATUS; CATION TRANSPORT PROTEINS; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; GENES, FUNGAL; ION TRANSPORT; MOLECULAR SEQUENCE DATA; MUTATION; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC; ZINC;

EID: 66349089052     PISSN: 11396709     EISSN: 16181905     Source Type: Journal    
DOI: 10.2436/20.1501.01.80     Document Type: Article

References (22)

1. Barrow NJ (1994) Mechanisms of reaction of zinc with soil and soil components. In: Robson AD (ed) Zinc in soils and plants. Springer, Heidelberg, pp 15-27
2. Bird AJ, Blankman E, Stillman DJ, Eide DJ, Winge DR (2004) The Zap1 transcriptional activator also acts as a repressor by binding downstream of the TATA box in ZRT2. EMBO J 23:1123-1132
3. Cove DJ (1966) The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochim Biophys Acta 113:51-56
4. Cove DJ (1976) Chlorate toxicity in Aspergillus nidulans. Studies of mutants altered in nitrate assimilation. Mol Gen Genet 146:147-159
5. d'Enfert C (1996) Selection of multiple disruption events in Aspergillus fumigatus using the orotidine-5'-decarboxylase gene, pyrG, as a unique transformation marker. Curr Genet 30:76-82
6. Espeso EA, Arst HN Jr. (2000) On the mechanism by which alkaline pH prevents expression of an acid-expressed gene. Mol Cell Biol 20:3355-3363
7. Fedorova ND, Khaldi N, Joardar VS, et al. (2008) Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus. PLoS Genet 4:e1000046
8. Gaither LA, Eide DJ (2001) Eukaryotic zinc transporters and their regulation. Biometals 14:251-270
9. Lamb TM, Mitchell AP (2003) The transcription factor Rim101p governs ion tolerance and cell differentiation by direct repression of the regulatory genes NRG1 and SMP1 in Saccharomyces cerevisiae. Mol Cell Biol 23:677-686
10. Lamb TM, Xu W, Diamond A, Mitchell AP (2001) Alkaline response genes of Saccharomyces cerevisiae and their relationship to the RIM101 pathway. J Biol Chem 276:1850-1856
11. Mingot JM, Tilburn J, Díez E, et al. (1999) Specificity determinants of proteolytic processing of Aspergillus PacC transcription factor are remote from the processing site, and processing occurs in yeast if pH signalling is bypassed. Mol Cell Biol 19:1390-1400
12. Moreno MA, Amich J, Vicentefranqueira R, Leal F, Calera JA (2007) Culture conditions for zinc- and pH-regulated gene expression studies in Aspergillus fumigatus. Int Microbiol 10:187-192
13. Moreno MA, Ibrahim-Granet O, Vicentefranqueira R, et al. (2007) The regulation of zinc homeostasis by the ZafA transcriptional activator is essential for Aspergillus fumigatus virulence. Mol Microbiol 64:1182-1197
14. Peñalva MA, Arst HN Jr. (2002) Regulation of gene expression by ambient pH in filamentous fungi and yeasts. Microbiol Mol Biol Rev 66:426-446
15. Peñalva MA, Arst HN Jr. (2004) Recent advances in the characterization of ambient pH regulation of gene expression in filamentous fungi and yeasts. Annu Rev Microbiol 58:425-451
16. Sambrook J, Russell DW (2001) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
17. Serrano R, Ruíz A, Bernal D, Chambers JR, Ariño J (2002) The transcriptional response to alkaline pH in Saccharomyces cerevisiae: evidence for calcium-mediated signalling. Mol Microbiol 46:1319-1333
18. Tilburn J, Sarkar S, Widdick DA, et al. (1995) The Aspergillus PacC zinc finger transcription factor mediates regulation of both acid- and alkaline-expressed genes by ambient pH. EMBO J 14:779-790
19. Vicentefranqueira R, Moreno MA, Leal F, Calera JA (2005) The zrfA and zrfB genes of Aspergillus fumigatus encode the zinc transporter proteins of a zinc uptake system induced in an acid, zinc-depleted environment. Eukaryot Cell 4:837-848
20. Zhao H, Eide D (1996) The yeast ZRT1 gene encodes the zinc transporter protein of a high-affinity uptake system induced by zinc limitation. Proc Natl Acad Sci USA 93:2454-2458
21. Zhao H, Eide D (1996) The ZRT2 gene encodes the low affinity zinc transporter in Saccharomyces cerevisiae. J Biol Chem 271:23203-23210
22. Zhao H, Eide DJ (1997) Zap1p, a metalloregulatory protein involved in zinc-responsive transcriptional regulation in Saccharomyces cerevisiae. Mol Cell Biol 17:5044-5052