Down-regulation of ZnT8 expression in INS-1 rat pancreatic beta cells reduces insulin content and glucose-inducible insulin secretion

PLoS ONE

Volumn 4, Issue 5, 2009, Pages

  Fu, Yi ^a,c   Tian, Wei ^a,c   Pratt, Emily B ^b   Dirling, Lisa B ^a,c   Shyng, Show Ling ^b   Meshul, Charles K ^a,c   Cohen, David M ^a,c  

^a OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

^b OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

^c PORTLAND VA MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GLUCOSE; INSULIN; MESSENGER RNA; PROTEIN SLC30A8; SHORT HAIRPIN RNA; UNCLASSIFIED DRUG; ZINC TRANSPORTER; ZINC TRANSPORTER 8; CATION TRANSPORT PROTEIN; NICKEL; QUINOLONE DERIVATIVE; SMALL INTERFERING RNA; TOLUENESULFONIC ACID DERIVATIVE; ZINC; ZINC TRANSPORTER ZNT8, RAT; ZINQUIN;

ANIMAL CELL; ARTICLE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; DNA POLYMORPHISM; GENE EXPRESSION REGULATION; GLYCEMIC CONTROL; HYPERGLYCEMIA; IMMUNOASSAY; INSULIN RELEASE; INSULIN RESPONSE; INSULIN SYNTHESIS; NONHUMAN; PANCREAS ISLET BETA CELL; PROTEIN EXPRESSION; PROTEIN FUNCTION; RAT; STIMULUS RESPONSE; ZINC METABOLISM; ANIMAL; CYTOLOGY; DOWN REGULATION; DRUG EFFECT; FLUORESCENCE; GENE SILENCING; GENETICS; INTRACELLULAR SPACE; METABOLISM; SECRETION; SECRETORY VESICLE; ULTRASTRUCTURE;

RATTUS;

ANIMALS; CATION TRANSPORT PROTEINS; DOWN-REGULATION; FLUORESCENCE; GENE KNOCKDOWN TECHNIQUES; GLUCOSE; INSULIN; INSULIN-SECRETING CELLS; INTRACELLULAR SPACE; NICKEL; QUINOLONES; RATS; RNA, MESSENGER; RNA, SMALL INTERFERING; SECRETORY VESICLES; TOSYL COMPOUNDS; ZINC;

EID: 66249128300     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0005679     Document Type: Article

References (40)

1. MacDonald PE, Rorsman P (2007) The ins and outs of secretion from pancreatic beta-cells: control of single-vesicle exo- and endocytosis. Physiology (Bethesda) 22: 113-21.
2. Chausmer AB (1998) Zinc, insulin and diabetes. J Am Coll Nutr 17: 109-15.
3. Sladek R, Rocheleau G, Rung J, Dina C, Shen L, et al. (2007) A genome-wide association study identifies novel risk loci for type 2 diabetes. Nature 445: 881-5.
4. Saxena R, Voight BF, Lyssenko V, Burtt NP, de Bakker PI, et al. (2007) Genome-Wide Association Analysis Identifies Loci for Type 2 Diabetes and Triglyceride Levels. Science 316: 1331-6.
5. Scott LJ, Mohlke KL, Bonnycastle LL, Willer CJ, Li Y, et al. (2007) A Genome-Wide Association Study of Type 2 Diabetes in Finns Detects Multiple Susceptibility Variants. Science 316: 1341-5.
6. Zeggini E, Weedon MN, Lindgren CM, Frayling TM, Elliott KS, et al. (2007) Replication of Genome-Wide Association Signals in U.K. Samples Reveals Risk Loci for Type 2 Diabetes. Science 317: 1336-41.
7. Steinthorsdottir V, Thorleifsson G, Reynisdottir I, Benediktsson R, Jonsdottir T, et al. (2007) A variant in CDKAL1 influences insulin response and risk of type 2 diabetes. Nat Genet 39: 770-5.
8. Chimienti F, Devergnas S, Favier A, Seve M (2004) Identification and cloning of a beta-cell-specific zinc transporter, ZnT-8, localized into insulin secretory granules. Diabetes 53: 2330-7.
9. Staiger H, Machicao F, Stefan N, Tschritter O, Thamer C, et al. (2007) Polymorphisms within novel risk loci for type 2 diabetes determine beta-cell function. PLoS ONE 2: e832.
10. Horikoshi M, Hara K, Ito C, Shojima N, Nagai R, et al. (2007) Variations in the HHEX gene are associated with increased risk of type 2 diabetes in the Japanese population. Diabetologia 50: 2461-6.
11. Omori S, Tanaka Y, Takahashi A, Hirose H, Kashiwagi A, et al. (2007) Association of CDKAL1, IGF2BP2, CDKN2A/B, HHEX, SLC30A8 and KCNJ11 with susceptibility to type 2 diabetes in a Japanese population. Diabetes 57: 791-5.
12. Cauchi S, Proenca C, Choquet H, Gaget S, De Graeve F, et al. (2008) Analysis of novel risk loci for type 2 diabetes in a general French population: the D.E.S.I.R. study. J Mol Med 86: 341-8.
13. Palmer ND, Goodarzi MO, Langefeld CD, Ziegler J, Norris JM, et al. (2008) Quantitative Trait Analysis of T2D Susceptibility Loci Identified from Whole Genome Association Studies in the IRAS Family Study. Diabetes.
14. Kirchhoff K, Machicao F, Haupt A, Schafer SA, Tschritter O, et al. (2008) Polymorphisms in the TCF7L2, CDKAL1 and SLC30A8 genes are associated with impaired proinsulin conversion. Diabetologia 51: 597-601.
15. Pascoe L, Tura A, Patel SK, Ibrahim IM, Ferrannini E, et al. (2007) Common variants of the novel type 2 diabetes genes CDKAL1 and HHEX/IDE are associated with decreased pancreatic beta-cell function. Diabetes 56: 3101-4.
16. Furukawa Y, Shimada T, Furuta H, Matsuno S, Kusuyama A, et al. (2008) Polymorphisms in the IDE-KIF11-HHEX gene locus are reproducibly associated with type 2 diabetes in a Japanese population. J Clin Endocrinol Metab 93: 310-4.
17. Wenzlau JM, Juhl K, Yu L, Moua O, Sarkar SA, et al. (2007) The cation efflux transporter ZnT8 (Slc30A8) is a major autoantigen in human type 1 diabetes. Proc Natl Acad Sci U S A 104: 17040-5.
18. Chimienti F, Devergnas S, Pattou F, Schuit F, Garcia-Cuenca R, et al. (2006) In vivo expression and functional characterization of the zinc transporter ZnT8 in glucose-induced insulin secretion. J Cell Sci 119: 4199-206.
19. Asfari M, Janjic D, Meda P, Li G, Halban PA, Wollheim CB (1992) Establishment of 2-mercaptoethanol-dependent differentiated insulin-secreting cell lines. Endocrinology 130: 167-78.
20. Zalewski PD, Forbes IJ, Betts WH (1993) Correlation of apoptosis with change in intracellular labile Zn(II) using zinquin [(2-methyl-8-p-toluenesulphonamido-6-quinolyloxy)acetic acid], a new specific fluorescent probe for Zn(II). Biochem J 296(Pt 2): 403-8.
21. Risch NJ (2000) Searching for genetic determinants in the new millennium. Nature 405: 847-56.
22. Yan H, Yuan W, Velculescu VE, Vogelstein B, Kinzler KW (2002) Allelic variation in human gene expression. Science 297: 1143.
23. Schadt EE, Monks SA, Drake TA, Lusis AJ, Che N, et al. (2003) Genetics of gene expression surveyed in maize, mouse and man. Nature 422: 297-302.
24. Hudson TJ (2003) Wanted: regulatory SNPs. Nat Genet 33: 439-40.
25. Lo HS, Wang Z, Hu Y, Yang HH, Gere S, et al. (2003) Allelic variation in gene expression is common in the human genome. Genome Res 13: 1855-62.
26. Morley M, Molony CM, Weber TM, Devlin JL, Ewens KG, et al. (2004) Genetic analysis of genome-wide variation in human gene expression. Nature 430: 743-7.
27. Buckland PR (2004) Allele-specific gene expression differences in humans. Hum Mol Genet 13 Spec No 2: R255-60.
28. Buckland PR (2006) The importance and identification of regulatory polymorphisms and their mechanisms of action. Biochim Biophys Acta 1762: 17-28.
29. Spielman RS, Bastone LA, Burdick JT, Morley M, Ewens WJ, Cheung VG (2007) Common genetic variants account for differences in gene expression among ethnic groups. Nat Genet 39: 226-31.
30. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(2Delta Delta C(T)) Method. Methods 25: 402-8.
31. Rayner MH, Suzuki KT (1995) A simple and effective method for the removal of trace metal cations from a mammalian culture medium supplemented with 10% fetal calf serum. Biometals 8: 188-92.
32. Min L, Leung YM, Tomas A, Watson RT, Gaisano HY, et al. (2007) Dynamin is functionally coupled to insulin granule exocytosis. J Biol Chem 282: 33530-6.
33. Liu G, Hilliard N, Hockerman GH (2004) Cav1.3 is preferentially coupled to glucose-induced [Ca2+]i oscillations in the pancreatic beta cell line INS-1. Mol Pharmacol 65: 1269-77.
34. Kambe T, Narita H, Yamaguchi-Iwai Y, Hirose J, Amano T, et al. (2002) Cloning and characterization of a novel mammalian zinc transporter, zinc transporter 5, abundantly expressed in pancreatic beta cells. J Biol Chem 277: 19049-55.
35. Sondergaard LG, Brock B, Stoltenberg M, Flyvbjerg A, Schmitz O, et al. (2005) Zinc fluxes during acute and chronic exposure of INS-1E cells to increasing glucose levels. Horm Metab Res 37: 133-9.
36. Frisk P, Tallkvist J, Gadhasson IL, Blomberg J, Friman G, Ilback NG (2007) Coxsackievirus B3 infection affects metal-binding/transporting proteins and trace elements in the pancreas in mice. Pancreas 35: e37-44.
37. Cousins RJ, Liuzzi JP, Lichten LA (2006) Mammalian zinc transport, trafficking, and signals. J Biol Chem 281: 24085-9.
38. Genome-wide association study of 14,000 cases of seven common diseases and 3,000 shared controls. Nature 447: 661-78.
39. Smidt K, Pedersen SB, Brock B, Schmitz O, Fisker S, et al. (2007) Zinc-transporter genes in human visceral and subcutaneous adipocytes: lean versus obese. Mol Cell Endocrinol 264: 68-73.
40. Coyle P, Zalewski PD, Philcox JC, Forbes IJ, Ward AD, et al. (1994) Measurement of zinc in hepatocytes by using a fluorescent probe, zinquin: relationship to metallothionein and intracellular zinc. Biochem J 303(Pt 3): 781-6.