Toward standardization of FoxP3+ regulatory T-cell measurement in clinical settings

Cytometry Part B - Clinical Cytometry

Volumn 76, Issue 2, 2009, Pages 67-68

  Lanza, F ^a  

^a UNIVERSITY HOSPITAL OF FERRARA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

HEPARIN; TRANSCRIPTION FACTOR FOXP3;

ANTICOAGULANT THERAPY; CANCER IMMUNOTHERAPY; CELL DIFFERENTIATION; CELL FUNCTION; CELL ISOLATION; CELL POPULATION; CULTURE TECHNIQUE; CYTOMETRY; EDITORIAL; FLOW CYTOMETRY; GRAFT VERSUS HOST REACTION; GRAFT VERSUS LEUKEMIA EFFECT; HUMAN; IMMUNOLOGICAL TOLERANCE; IMMUNOPHENOTYPING; MOLECULAR PROBE; NONHUMAN; PATHOGENESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REGULATORY T LYMPHOCYTE; STANDARDIZATION; VALIDATION PROCESS;

ANIMALS; CRYOPRESERVATION; FLOW CYTOMETRY; FORKHEAD TRANSCRIPTION FACTORS; HUMANS; IMMUNOPHENOTYPING; IMMUNOSUPPRESSION; SELF TOLERANCE; STAINING AND LABELING; STEM CELL TRANSPLANTATION; T-LYMPHOCYTES, REGULATORY; TISSUE FIXATION;

EID: 66249113560     PISSN: 15524949     EISSN: 15524957     Source Type: Journal    
DOI: 10.1002/cyto.b.20471     Document Type: Editorial

References (8)

1. Le NT, Chao N. Regulating regulatory T cells. Bone Marrow Transplant 2007;39:1-9.
2. de kleer I, Vastert B, Klein M, Teklenburg G, Arkesteijin G, Yung G, Albani S, Kuis W, Wulffraaat N, Prakken B. Autologous stem cell transplantation for autoimmunity induces immunologic self tolerance by reprogramming autoreactive T-cells and restoring the CD4+CD25+ immune regulatory network. Blood 2006;107:1696-1702.
3. Colombo M, Piconese S. Regulatory T-cell inhibition versus depletion: The right choice in cancer immunotherapy. Nature 2007;7: 880-887.
4. Chen B. T-reg-cell expansion: Better to be naive. Blood 2006;108: 3963-3964.
5. Grant J, Bourcier K, Wallace S, Pan D, Conway A, Seyfert-Margolis V, Wallace P. Validated protocol for Foxp3 reveals increased expression in type 1 diabetes patients. Cytometry Part B 2009;76B:69-78 (this issue).
6. Lanza F, Latorraca A, Moretti S, Castagnari B, Ferrari L, Castoldi GL. Comparative analysis of different permeabilization methods for the flow cytometry measurement of cytoplasmic MPO and lysozyme in normal and leukemic cells. Cytometry 1997;30:1-11.
7. O'Gorman M, Thomas J. Isotype controls-time to let go? Cytometry 1999;38:78-80.
8. Hoffmann P, Boeld TJ, Eder R, Huehn J, Floess S, Wieczorek G, Olek S, Andreesen R, Edinger M. Loss of Foxp3 expression after in vitro expansion of human CD4+CD25_CD127- regulatory T-cells. Bone Marrow Transplant 2008;41:s58.