Isolation of rpoB mutations causing rifampicin resistance in bacillus subtilis spores exposed to simulated martian surface conditions

Astrobiology

Volumn 8, Issue 6, 2008, Pages 1159-1167

  Perkins, Amy E ^a   Schuerger, Andrew C ^a   Nicholson, Wayne L ^a,b  

^a UNIVERSITY OF FLORIDA   (United States)

^b KENNEDY SPACE CENTER   (United States)

Author keywords

Bacillus subtilis; Mars; Mutagenesis; Rifampicin; RNA polymerase; RpoB; Spores

Indexed keywords

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS);

BACTERIAL PROTEIN; RIFAMPICIN;

ANTIBIOTIC RESISTANCE; ARTICLE; ASTRONOMY; BACILLUS SUBTILIS; BACTERIAL SPORE; CYTOLOGY; DRUG EFFECT; ETHNOLOGY; GENETICS; MICROBIAL VIABILITY; MICROCLIMATE; MOLECULAR GENETICS; MUTAGENESIS; MUTATION; NUCLEOTIDE SEQUENCE; SPACE FLIGHT;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; BASE SEQUENCE; DRUG RESISTANCE, MICROBIAL; EARTH (PLANET); EXTRATERRESTRIAL ENVIRONMENT; MARS; MICROBIAL VIABILITY; MOLECULAR SEQUENCE DATA; MUTAGENESIS; MUTATION; RIFAMPIN; SPACE SIMULATION; SPORES, BACTERIAL;

EID: 66149184683     PISSN: 15311074     EISSN: None     Source Type: Journal    
DOI: 10.1089/ast.2007.0224     Document Type: Article

References (51)

1. Benardini, J.N., Sawyer, J., Venkateswaran, K., and Nicholson, W.L. (2003) Spore UV and acceleration resistance of endolithic Bacillus pumilus and Bacillus subtilis isolates obtained from Sonoran desert basalt: implications for lithopanspermia. Astrobiology 3:709-717.
2. Campbell, E.A., Korzheva, N., Mustaev, A., Murakami, K., Nair, S., Goldfarb, A., and Darst, S.A. (2001) Structural mechanism for rifampicin inhibition of bacterial RNA polymerase. Cell 104:901-912.
3. Clark, B. (2001) Planetary interchange of bioactive material: probability factors and implications. Orig. Life Evol. Biosph. 31:185-197.
4. Crawford, R. (2005) Microbial diversity and its relationship to planetary protection. Appl. Environ. Microbiol. 71:4163-4168.
5. Cutting, S.M. and Vander Horn, P.B. (1990) Genetic techniques. In Molecular Biological Methods for Bacillus, edited by C.R. Harwood and S.M. Cutting, John Wiley & Sons, Sussex, England, pp 27-74.
6. del Carmen Huesca-Espitia, L., Caley, C., Bagyan, I., and Setlow, P. (2002) Base-change mutations induced by various treatments of Bacillus subtilis spores with and without DNA protective small, acid-soluble spore proteins. Mutat. Res. 503:77-84.
7. Dose, K., Bieger-Dose, A., Kerz, O., and Gill, M. (1991) DNA-strand breaks limit survival in extreme dryness. Orig. Life Evol. Biosph. 21:177-188.
8. Dose, K., Bieger-Dose, A., Labusch, M., and Gill, M. (1992) Survival in extreme dryness and DNA-single-strand breaks. Adv. Space Res. 12:221-229.
9. Fajardo-Cavazos, P. and Nicholson, W.L. (2006) Bacillus en-dospores isolated from granite: close molecular relationships to globally distributed Bacillus spp. from endolithic and extreme environments. Appl. Environ, Microbiol. 72:2856-2863.
10. Fajardo-Cavazos, P., Schuerger, A.C., and Nicholson, W.L. (2007) Testing interplanetary transfer of bacteria by natural impact phenomena and human spaceflight activities. Acta Astronaut. 60:534-540.
11. Horneck, G. (1993) Responses of Bacillus subtilis spores to the space environment: results from experiments in space. Orig. Life Evol. Biosph. 23:37-52.
12. Horneck, G., Bücker, H., Reitz, G., Requardt, H., Dose, K., Martens, K.D., Menningmann, H.D., and Weber, P. (1984) Microorganisms in the space environment. Science 225: 226-228.
13. Horneck, G., Miliekowsky, C., Melosh, H.J., Wilson, J.W., Cu-cinotta, F.A., and Gladman, B. (2002) Viable transfer of microorganisms in the solar system and beyond. In Astrobiology: the Quest for the Conditions of Life, edited by G. Horneck and C. Baumstark-Khan, Springer, Berlin, pp 55-76.
14. Kempf, M.J., Chen, F., Kern, R., and Venkateswaran, K. (2005) Recurrent isolation of hydrogen peroxide-resistant spores of Bacillus pumilus from a spacecraft assembly facility. Astrobiol-ogy 5:391-405.
15. La Duc, M.T., Nicholson, W.L., Kern, R., and Venkateswaran, K. (2003) Microbial characterization of the Mars Odyssey spacecraft and its encapsulation facility. Environ. Microbiol. 5:977-985.
16. Lindberg, C. and Horneck, G. (1991) Action spectra for survival and spore photoproduct formation of Bacillus subtilis irradiated with short-wavelength (200-300 nm) UV at atmospheric pressure and in vacuo. J. Photochem. Photobiol. B., Biol. 11:69-80.
17. Lindberg, C. and Horneck, G. (1992) Thymine photoproduct formation and inactivation of intact spores of Bacillus subtilis irradiated with short wavelength UV (200-300 nm) at atmospheric pressure and in vacuo. Adv. Space Res. 12:275-279.
18. Link, L., Sawyer, J., Venkateswaran, K., and Nicholson, W.L. (2004) Extreme spore UV resistance of Bacillus pumilus isolates obtained from an ultra-clean spacecraft assembly facility. Microb. Ecol. 47:159-163.
19. Mastrapa, R.M.E., Glanzberg, H., Head, J.N., Melosh, H.J., and Nicholson, W.L. (2001) Survival of bacteria exposed to extreme acceleration: implications for panspermia. Earth Planet. Sci. Lett. 189:1-8.
20. Maughan, H., Galeano, B., and Nicholson, W.L. (2004) Novel rpoB mutations conferring rifampicin resistance on Bacillus subtilis: global effects on growth, competence, sporulation, and germination. J. Bacteriol. 186:2481-2486.
21. Mileikowsky, C., Cucinotta, F.A., Wilson, J.W., Gladman, B., Horneck, G., Lindegren, L., Melosh, H.J., Rickman, H., Valtonen, M., and Zheng, J.Q. (2000) Natural transfer of viable microbes in space. Part 1: From Mars to Earth and Earth to Mars. Icarus 145:391-427.
22. Miller, J. (1972) Experiments in Molecular Genetics, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
23. Munakata, N., Saito, M., Takahashi, N., Hieda, K., and Morihoshi, F. (1997) Induction of unique tandem-base change mutations in bacterial spores exposed to extreme dryness. Mutat. Res. 390:189-195.
24. Munakata, N., Maeda, M., and Hieda, K. (2002) Vacuum-induced mutations in Bacillus subtilis spores [abstract EGS02-A-02047]. In Abstracts of the 27th General Assembly of the European Geophysical Society, Nice, France, European Geophysical Society.
25. Munakata, N., Natsume, T., Takahashi, K., Hieda, K., Panitz, C., and Horneck, G. (2004) Mutagenesis of Bacillus subtilis spores exposed to simulated space environment [abstract 04-A-00898]. In Abstracts of the 35th COSPAR Scientific Assembly, COSPAR, Paris.
26. Newcombe, D.A., Schuerger, A.C., Benardini, J.N., Dickinson, D., Tanner, R., and Venkateswaran, K. (2005) Survival of spacecraft-associated microorganisms under simulated martian UV irradiation. Appl. Environ. Microbiol. 71:8147-8156.
27. Nicholson, W.L. (2002) Roles of Bacillus spores in the environment. Cell. Mol. Life Sci. 59:410-416.
28. Nicholson, W.L. (2004) Ubiquity, longevity, and ecological roles of Bacillus spores. In Bacterial Spore Formers: Probiotics and Emerging Applications, edited by E. Ricca, A.O. Henriques, and S.M. Cutting, Horizon Scientific Press, Norfolk, UK, pp 1-15.
29. Nicholson, W.L. and Law, J.F. (1999) Method for purification of bacterial endospores from soils: UV resistance of natural Sono-ran desert soil populations of Bacillus spp. with reference to B. subtilis strain 168. J. Microbiol. Methods 35:13-21.
30. Nicholson, W.L. and Maughan, H. (2002) The spectrum of spontaneous rifampicin resistance mutations in the rpoB gene of Bacillus subtilis 168 spores differs from vegetative cells and resembles that of Mycobacterium tuberculosis. J. Bacteriol. 184:4936-4940.
31. Nicholson, W.L. and Schuerger, A.C. (2005) Survival and germinability of Bacillus subtilis spores after incubation at Mars atmospheric pressure: implications for planetary protection and lithopanspermia. Astrobiology 5:536-544.
32. Nicholson, W.L. and Setlow, P. (1990) Sporulation, germination, and outgrowth. In Molecular Biological Methods for Bacillus, edited by C.R. Harwood and S.M. Cutting, John Wiley & Sons, Sussex, England, pp 391-450.
33. Nicholson, W.L., Munakata, N., Horneck, G., Melosh, H.J., and Setlow, P. (2000) Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments. Microbiol. Mol. Biol. Rev. 64:548-572.
34. Nicholson, W.L., Setlow, B., and Setlow, P. (2002) UV photochemistry of DNA in vitro and in Bacillus subtilis spores at Earth-ambient and low atmospheric pressure: implications for spore survival on other planets or moons in the Solar System. Astrobiology 2:417-425.
35. Nicholson, W.L., Schuerger, A.C., and Setlow, P. (2005) The solar UV environment and bacterial spore UV resistance: considerations for Earth-to-Mars transport by natural processes and human spaceflight. Mutat. Res. 571:249-264.
36. Owen, T. (1992) The composition and early history of the atmosphere of Mars. In Mars, edited by H.H. Kieffer, B.M. Jakosky, C.W. Snyder, and M.S. Mathews, University of Arizona Press, Tucson, AZ, pp 818-834.
37. Perkins, A.E. and Nicholson, W.L. (2008) Uncovering new metabolic capabilities of Bacillus subtilis using phenotype profiling of rifampicin-resistant rpoB mutants. J. Bacteriol. 190:807-814.
38. Qi, Y. and Hulett, F.M. (1998) PhoP∼P and RNA polymerase sigma A holoenzyme are sufficient for transcription of Pho regulon promoters in Bacillus subtilis: PhoP∼P activator sites within the coding region stimulate transcription in vitro. Mol. Microbiol. 28:1187-1198.
39. Schaeffer, P., Millet, J., and Aubert, J.-P. (1965) Catabolic repression of bacterial sporulation. Proc. Natl. Acad. Sci. U.S.A. 54:704-711.
40. Schuerger, A.C. (2004) Microbial ecology of the surface exploration of Mars with human-operated vehicles. In Martian Expedition Planning, edited by C.S. Cockell, American Astronautical Society publication AAS 03-322, Univelt Publishers, Santa Barbara, CA, pp 363-386.
41. Schuerger, A.C. and Nicholson, W.L. (2006) Interactive effects of hypobaria, low temperature, and CO2 atmosphere inhibit the growth of mesophilic Bacillus spp. under simulated martian conditions. Icarus 185:143-152.
42. Schuerger, A.C, Mancinelli, R.L., Kern, R.G., Rothschild, L.J., and McKay, C.P. (2003) Survival of endospores of Bacillus subtilis on spacecraft surfaces under simulated martian environments: implications for the forward contamination of Mars. Icarus 165:253-276.
43. Schuerger, A.C, Berry, B., and Nicholson, W.L. (2006) Terrestrial bacteria typically recovered from Mars spacecraft do not appear able to grow under simulated martian conditions [abstract 1397]. In 37th Lunar and Planetary Science Conference Abstracts, LPI contribution No. 1303, Lunar and Planetary Institute, Houston.
44. Schuerger, A.C, Farjardo-Cavazos, P., Clausen, C.A., Moores, J.E., Smith, P.H., and Nicholson, W.L. (2008) Slow degradation of ATP in simulated martian environments suggests long residence times for the biosignature molecule on spacecraft surfaces on Mars. Icarus 194:86-100.
45. Severinov, K, Soushko, M., Goldfarb, A., and Nikiforov, V. (1993) Rifampicin region revisited: new rifampicin-resistant and streptolydigin- resistant mutants in the /3 subunit of Escherichia coli RNA polymerase. J. Biol. Chem. 268:13820-14825.
46. Spizizen, J. (1958) Transformation of biochemically deficient strains of Bacillus subtilis by deoxyribonucleate. Proc. Natl. Acad. Sci. U.S.A. 44:1072-1078.
47. Tauscher, C., Schuerger, A.C., and Nicholson, W.L. (2006) Survival and germinability of Bacillus subtilis spores exposed to simulated Mars solar radiation: implications for life detection and planetary protection. Astrobiology 6:592-605.
48. Tillman, J.E. (1988) Mars global atmospheric oscillations: annually synchronized, transient normal-mode oscillations and the triggering of global dust storms. J. Geophys. Res. 93:9433-9451.
49. Venkateswaran, K., Satomi, M., Chung, S., Kern, R., Koukol, R., Basic, C., and White, D. (2001) Molecular microbial diversity of a spacecraft assembly facility. Syst. Appl. Microbiol. 24:311-320.
50. Venkateswaran, K., Kempf, M., Chen, F., Satomi, M., Nicholson, W.L., and Kern, R. (2003) Bacillus nealsonii sp. nov., isolated from a spacecraft-assembly facility, whose spores are gamma-radiation resistant. Int. J. Syst. Evol. Microbiol. 53:165-172
51. Yatagai, F., Saito, T., Takahashi, A., Fujie, A., Nagaoka, S., Sato, M., and Ohnishi, T. (2000) rpsL mutation induction after space flight on MIR. Mutat. Res. 453:1-4.