Measurement of phosphorylated extracellular signal - Regulated kinase 1 and 2 in an undergraduate teaching laboratory with alphascreen technology

Science Signaling

Volumn 2, Issue 62, 2009, Pages

  Hay, Debbie L ^a  

^a UNIVERSITY OF AUCKLAND   (New Zealand)

Author keywords

[No Author keywords available]

Indexed keywords

MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; CALCITONIN; MICROSPHERE;

EXCITATION; HIGH THROUGHPUT SCREENING; LABORATORY; MEDICAL EDUCATION; MEDICAL TECHNOLOGY; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; SHORT SURVEY; SIGNAL TRANSDUCTION; ARTICLE; BIOCHEMISTRY; DIAGNOSIS, MEASUREMENT AND ANALYSIS; EDUCATION; METABOLISM; METHODOLOGY; PHOSPHORYLATION; PHYSIOLOGY; TEACHING;

BIOCHEMISTRY; CALCITONIN; EDUCATION, MEDICAL, UNDERGRADUATE; LABORATORY TECHNIQUES AND PROCEDURES; MICROSPHERES; MITOGEN-ACTIVATED PROTEIN KINASE 1; MITOGEN-ACTIVATED PROTEIN KINASE 3; PHOSPHORYLATION; SIGNAL TRANSDUCTION; TEACHING MATERIALS;

EID: 66149142213     PISSN: 19450877     EISSN: 19379145     Source Type: Journal    
DOI: 10.1126/scisignal.262tr1     Document Type: Short Survey

References (6)

1. R. V. Blystone, B. MacAlpine, WWW.Cell biology education: Using the world wide web to develop a new teaching topic. Cell Biol. Educ. 4, 105-111 (2005).
2. AlphaScreen SureFire Cellular Kinase Assays. Brochure, PerkinElmer (2008); http://las.perkinelmer.com/content/RelatedMaterials/Brochures/FLY- AlphaScreenSureFireCellularKinaseAssays.pdf.
3. R. M. Eglen, R. Bosse, T. Reisine, Emerging concepts of guanine nucleotide-binding protein-coupled receptor (GPCR) function and implications for high throughput screening. Assay Drug Dev. Technol. 5, 425-451 (2007).
4. S. Siehler, Cell-based assays in GPCR drug discovery. Biotechnol. J. 3, 471-483 (2008).
5. Protocol for AlphaScreen SureFire® p-ERK Assay Kits. PerkinElmer (24 June 2008); http://las.perkinelmer.com/content/Manuals/MAN- AlphaScreenSureFirepERK.pdf.
6. I thank PerkinElmer and TGR Biosciences for their generous sponsorship of this teaching laboratory. I also thank I. Liang for her in-class technical assistance.