A functional role for transposases in a large eukaryotic genome

Science

Volumn 324, Issue 5929, 2009, Pages 935-938

  Nowacki, Mariusz ^a   Higgins, Brian P ^a   Maquilan, Genevieve M ^a   Swart, Estienne C ^a   Doak, Thomas G ^b   Landweber, Laura F ^a  

^a PRINCETON UNIVERSITY   (United States)

^b INDIANA UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENOMIC DNA; RNA; TRANSPOSASE; PROTOZOAL DNA;

DNA; ENZYME ACTIVITY; EUKARYOTE; GENE EXPRESSION; GENOME; RNA;

ARTICLE; CILIATE; EUKARYOTE; GENE; GENE EXPRESSION; GENE REARRANGEMENT; GENOME; GERM LINE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROGENY; RNA INTERFERENCE; TRANSPOSON; AMINO ACID SEQUENCE; ANIMAL; BACTERIUM CONJUGATION; CHEMISTRY; ENZYMOLOGY; GENE DELETION; GENETICS; GERMLINE MICRONUCLEUS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; MOLECULAR GENETICS;

EUKARYOTA; OXYTRICHA; STERKIELLA HISTRIOMUSCORUM;

AMINO ACID SEQUENCE; ANIMALS; CONJUGATION, GENETIC; DNA TRANSPOSABLE ELEMENTS; DNA, PROTOZOAN; GENE EXPRESSION; GENE REARRANGEMENT; GENOME, PROTOZOAN; MICRONUCLEUS, GERMLINE; MOLECULAR SEQUENCE DATA; OXYTRICHA; RNA INTERFERENCE; SEQUENCE DELETION; TRANSPOSASES;

EID: 66149132301     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1170023     Document Type: Article

References (23)

1. D. M. Prescott, Microbiol. Rev. 58, 233 (1994).
2. D. L. Chalker, M. C. Yao, Mol. Cell. Biol. 16, 3658 (1996).
3. S. Duharcourt, A. M. Keller, E. Meyer, Mol. Cell. Biol. 18, 7075 (1998).
4. K. Mochizuki, N. A. Fine, T. Fujisawa, M. A. Gorovsky, Cell 110, 689 (2002).
5. M. Nowacki et al., Nature 451, 153 (2008).
6. D. J. Witherspoon et al., Mol. Biol. Evol. 14, 696 (1997).
7. G. Herrick et al., Cell 43, 759 (1985).
8. T. G. Doak, F. P. Doerder, C. L. Jahn, G. Herrick, Proc. Natl. Acad. Sci. U.S.A. 91, 942 (1994).
9. Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr. A. Seegmiller et al., Mol. Biol. Evol. 13, 1351 (1996).
10. K. Williams, T. G. Doak, G. Herrick, EMBO J. 12, 4593 (1993).
11. L. A. Klobutcher, G. Herrick, Prog. Nucleic Acid Res. Mol. Biol. 56, 1 (1997).
12. D. J. Hunter, K. Williams, S. Carinhour, G. Herrick, Genes Dev. 3, 2101 (1989).
13. W. F. Doolittle, C. Sapienza, Nature 284, 601 (1980).
14. L. E. Orgel, F. H. Crick, Nature 284, 604 (1980).
15. T. G. Doak, D. J. Witherspoon, F. P. Doerder, K. Williams, G. Herrick, Genetica 101, 75 (1997).
16. M. Morgante, P. De Paoli, S. Radovic, Curr. Opin. Plant Biol. 10, 149 (2007).
17. M. G. Kidwell, A. J. Lish, Evolution 55, 1 (2001).
18. V. V. Kapitonov, J. Jurka, PLoS Biol. 3, e 181 (2005).
19. A. Sarkar et al., Mol. Genet. Genomics 270, 173 (2003).
20. H. M. Robertson, K. L. Zumpano, Gene 205, 203 (1997).
21. M. Mollenbeck et al., PLoS One 3, e2330 (2008).
22. Materials and methods are available as supporting material on Science Online.
23. This work was supported by NSF grant 0622112 and NIH grant GM59708. We thank J. Wang for technical assistance. DNA sequences have been deposited in GenBank under accession numbers FJ666213-FJ666314 (TBE transposases), FJ545743 [ribosomal DNA (rDNA) macronucleus], and FJ545744 (rDNA micronucleus).