Synthesis, characterization, and immunogenicity in mice of Shigella sonnei O-specific oligosaccharide-core-protein conjugates

Proceedings of the National Academy of Sciences of the United States of America

Volumn 106, Issue 19, 2009, Pages 7974-7978

  Robbins, John B ^a   Kubler Kielb, Joanna ^a   Vinogradov, Evguenii ^b   Mocca, Christopher ^a   Pozsgay, Vince ^a   Shiloach, Joseph ^c   Schneerson, Rachel ^a  

^a EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^c NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

Author keywords

Glycoconjugate; IgG; Kdo; Lipopolysaccharide; Vaccine

Indexed keywords

BACTERIAL VACCINE; BOVINE SERUM ALBUMIN; DIPHTHERIA TOXIN; GLYCOCONJUGATE; IMMUNOGLOBULIN FRAGMENT; IMMUNOGLOBULIN G; IMMUNOGLOBULIN G ANTIBODY; O SPECIFIC OLIGOSACCHARIDE CORE PROTEIN CONJUGATE VACCINE; OXIME; SHIGELLA VACCINE; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANTIBODY BLOOD LEVEL; ARTICLE; CONTROLLED STUDY; DRUG INHIBITION; DRUG SYNTHESIS; EXPERIMENTAL MOUSE; FEMALE; IMMUNIZATION; IMMUNOGENICITY; INFECTION RATE; MOUSE; NONHUMAN; PH; PREVENTION AND CONTROL; PRIORITY JOURNAL; PROTEIN SYNTHESIS; ROOM TEMPERATURE; SHIGELLA SONNEI;

ANIMALS; ANTIBODIES; BACTERIAL VACCINES; DYSENTERY, BACILLARY; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; GLYCOCONJUGATES; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN G; LIPOPOLYSACCHARIDES; MAGNETIC RESONANCE SPECTROSCOPY; MICE; O ANTIGENS; SHIGELLA SONNEI; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

MUS; SHIGELLA DYSENTERIAE; SHIGELLA SONNEI;

EID: 66049158947     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0900891106     Document Type: Article

References (21)

1. Szu SC, Robbins JB, Schneerson R, Pozsgay V, Chu C (2004) in New Generation Vaccines, eds Levine MM, Kaper JB, Rappuoli R, Liu MA, Good M (Dekker, New York), pp 471-478.
2. Ferreccio C, et al. (1991) Epidemiologic patterns of acute diarrhea and endemic Shigella infections in children in a poor periurban setting in Santiago, Chile. Am J Epidemiol 134:614-627.
3. Robbins JB, Chu C, Schneerson R (1992) Hypothesis for vaccine development: Protective immunity to enteric diseases caused by nontyphoidal salmonellae and shigellae may be conferred by serum IgG antibodies to the O-specific polysaccharide of their lipopolysaccharides. Clin Infect Dis 2:346-361.
4. Claesson B, et al. (2005) Antibodies against Haemophilus influenzae type b capsular polysaccharide and tetanus toxoid before and after a booster dose of the carrier protein 9 years after primary vaccination with a protein conjugate vaccine. Pediatr Infect Dis J 24:463-464.
5. Taylor DN, et al. (1993) Synthesis, characterization, and clinical evaluation of conjugate vaccines composed of the O-specific polysaccharides of Shigella dysenteriae type 1, Shigella flexneri type 2a, and Shigella sonnei (Plesiomonas shigelloides) bound to bacterial toxoids. Infect Immun 61:3678-3687.
6. Cohen D, et al. (1997) Double-blinded vaccine-controlled randomized efficacy trial of an investigational Shigella sonnei conjugate vaccine in young adults. Lancet 349:155-159.
7. succ conjugate vaccines in 1- to 4-year-old children. Pediatr Inf Dis J 22:701-706.
8. Pozsgay V, et al. (1999) Protein conjugates of synthetic saccharides elicit higher levels of serum IgG lipopolysaccharide antibodies in mice than do those of the O-specific polysaccharide from Shigella dysenteriae type 1. Proc Natl Acad Sci USA 96:5194-5197.
9. Kenne L, Lindberg B, Petersson K, Katzenellenbogen E, Romanowska E (1980) Structural studies of the O-specific side-chains of the Shigella sonnei phase I lipopolysaccharide. Carbohydr Res 78:119-126.
10. Gamian A, Romanowaska E (1982) The core structure of Shigella sonnei lipopolysaccharide and the linkage between O-specific polysaccharide and the core region. Eur J Biochem 129:105-109.
11. Kubler-Kielb J, et al. (2008) Saccharide/protein conjugate vaccines for Bordetella species: Preparation of saccharide, development of new conjugation procedures, and physico-chemical and immunological characterization of the conjugates. Vaccine 26:3587-3593.
12. Kubler-Kielb J, Schneerson R, Mocca C, Vinogradov E (2008) The elucidation of the structure of the core part of the LPS from Plesiomonas shigelloides serotype O17 expressing O-polysaccharide chain identical to the Shigella sonnei O-chain. Carbohydr Res 343:2701-2705.
13. Tóth A, et al. (2000) Synthesis of the repeating unit of the O-specific polysaccharide of Shigella sonnei and quantitation of its serologic activity. Bioorg Med Chem Lett 10:19-21.
14. Pozsgay V, Kubler-Kielb J, Schneerson R, Robbins JB (2007) Effect of the nonreducing end of Shigella dysenteriae type 1 O-specific oligosaccharides on their immunogenicity as conjugates in mice. Proc Natl Acad Sci USA 104:14478-14482.
15. Westphal O, Jann K, Himmelspach K (1983) Chemistry and immunochemistry of bacterial lipopolysaccharides as cell wall antigens and endotoxins. Prog Allergy 33:9-39.
16. Lowry JO, Rosenbrough NJ, Farr AL, Randall R (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265-275.
17. Scott TA, Melvin EH (1953) Determination of dextran with anthrone. Anal Chem 25:1656-1661.
18. Johnson VG, Nicholls PJ (1994) Histidine 21 does not play a major role in diphtheria toxin catalysis. J Biol Chem 269:4349-4354.
19. Schneerson R, Barrera O, Sutton A, Robbins JB (1980) Preparation, characterization, and immunogenicity of Haemophilus influenzae type b polysaccharide-protein conjugates. J Exp Med 152:361-376.
20. Plikaytis BD, Carlone GM (2005) Program ELISA for Windows User's Manual (Centers for Disease Control, Atlanta), Version 2.
21. Preston A, et al. (2006) Complete structure of Bordetella bronchiseptica and Bordetella parapertussis lipopolysaccharides. J Biol Chem 281:18135-18144.