Virus-induced gene silencing as a tool for delivery of dsRNA into plants

Cold Spring Harbor Protocols

Volumn 4, Issue 2, 2009, Pages

  Padmanabhan, Meenu ^a   Dinesh Kumar, Savithramma P ^a  

^a YALE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED RNA; OXYGENASE; PHYTOENE DESATURASE; UNCLASSIFIED DRUG; VIRUS VECTOR;

ARABIDOPSIS; ARTICLE; BACTERIUM CULTURE; CONTROLLED STUDY; GENE INSERTION; GENE SILENCING; INOCULATION; NICOTIANA BENTHAMIANA; NONHUMAN; PLANT; RHIZOBIUM RADIOBACTER; TOMATO; VIRAL GENE DELIVERY SYSTEM; VIRUS INDUCED GENE SILENCING;

EID: 65749114440     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5139     Document Type: Article

References (12)

1. Baulcombe, D.C. 1999. Fast forward genetics based on virus-induced gene silencing. Curr. Opin. Plant Biol. 2: 109-113.
2. Burch-Smith, T.M., Anderson, J.C., Martin, G.B., and Dinesh-Kumar, S.P. 2004. Applications and advantages of virus-induced gene silencing for gene function studies in plants. Plant J. 39: 734-746.
3. Burch-Smith, T.M., Miller, J.L., and Dinesh-Kumar, S.P. 2006a. Delivery of dsRNA into plants by VIGS methodology. Cold Spring Harb. Protoc. doi: 10.1101/pdb.prot4327.
4. Burch-Smith, T.M., Schiff, M., Liu, Y., and Dinesh-Kumar, S.P. 2006b. Efficient virus-induced gene silencing in Arabidopsis. Plant Physiol. 142: 21-27.
5. Constantin, G.D., Krath, B.N., MacFarlane, S.A., Nicolaisen, M., Johansen, I.E., and Lund, O.S. 2004. Virus-induced gene silencing as a tool for functional genomics in a legume species. Plant J. 40: 622-631.
6. Ding, X.S., Schneider, W.L., Chaluvadi, S.R., Mian, M.A., and Nelson, R.S. 2006. Characterization of a Brome mosaic virus strain and its use as a vector for gene silencing in monocotyledonous hosts. Mol. Plant Microbe Interact. 19: 1229-1239.
7. Fofana, I.B., Sangaré, A., Collier, R., Taylor, C., and Fauquet, C.M. 2004. A geminivirus-induced gene silencing system for gene function validation in cassava. Plant Mol. Biol. 56: 613-624.
8. Liu, Y., Schiff, M., Marathe, R., and Dinesh-Kumar, S.P. 2002. Tobacco Rar1, EDS1 and NPR1/NIM1 like genes are required for N-mediated resistance to tobacco mosaic virus. Plant J. 30: 415-429.
9. Lu, R., Martin-Hernandez, A.M., Peart, J.R., Malcuit, I., and Baulcombe, D.C. 2003. Virus-induced gene silencing in plants. Methods 30: 296-303.
10. Pflieger, S., Blanchet, S., Camborde, L., Drugeon, G., Rousseau, A., Noizet, M., Planchais, S., and Jupin, I. 2008. Efficient virus-induced gene silencing in Arabidopsis using a 'one-step' TYMV-derived vector. Plant J. 56: 678-690.
11. Ratcliff, F., Martin-Hernandez, A.M., and Baulcombe, D.C. 2001. Tobacco rattle virus as a vector for analysis of gene function by silencing. Plant J. 25: 237-245.
12. Wang, M.B. and Waterhouse, P.M. 2002. Application of gene silencing in plants. Curr. Opin. Plant Biol. 5: 146-150.