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Cold Spring Harbor Protocols

Volumn 4, Issue 2, 2009, Pages

In-gel trypsin digest of gel-fractionated proteins

(2) Link, Andrew J a LaBaer, Joshua b

a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE (United States)

b HARVARD MEDICAL SCHOOL (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYSTEINE; POLYACRYLAMIDE GEL; REAGENT; TRYPSIN;

ALKYLATION; ARTICLE; LIQUID CHROMATOGRAPHY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN PURIFICATION; QUANTITATIVE ANALYSIS; SEPARATION TECHNIQUE; SOLVENT EXTRACTION; STAINING;

EID: 65749096089 PISSN: None EISSN: 15596095 Source Type: Journal
DOI: 10.1101/pdb.prot5110 Document Type: Article

Times cited : (14)

References (8)

1
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- Absolute quantification of proteins in solutions and in polyacrylamide gels by mass spectrometry
- Havlis, J. and Shevchenko, A. 2004. Absolute quantification of proteins in solutions and in polyacrylamide gels by mass spectrometry. Anal. Chem. 76: 3029-3036.
- (2004) Anal. Chem , vol.76 , pp. 3029-3036
- Havlis, J.¹ Shevchenko, A.²

2
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- Fast-response proteomics by accelerated in-gel digestion of proteins
- Havlis, J., Thomas, H., Sebela, M., and Shevchenko, A. 2003. Fast-response proteomics by accelerated in-gel digestion of proteins. Anal. Chem. 75: 1300-1306.
- (2003) Anal. Chem , vol.75 , pp. 1300-1306
- Havlis, J.¹ Thomas, H.² Sebela, M.³ Shevchenko, A.⁴

3
- 65749090094
- Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
- Link, A.J. and LaBaer, J. 2009. Proteomics: A Cold Spring Harbor Laboratory course manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
- (2009) Proteomics: A Cold Spring Harbor Laboratory course manual
- Link, A.J.¹ LaBaer, J.²

4
- 65749083019
- SDS-polyacrylamide gel electrophoresis of proteins
- doi: 10.1101/pdb.prot4540
- Sambrook, J. and Russell, D.W. 2006. SDS-polyacrylamide gel electrophoresis of proteins. Cold Spring Harb. Protoc. doi: 10.1101/pdb.prot4540.
- (2006) Cold Spring Harb. Protoc
- Sambrook, J.¹ Russell, D.W.²

5
- 0035884102
- Evaluation of the efficiency of in-gel digestion of proteins by peptide isotopic labeling and MALDI mass spectrometry
- Shevchenko, A. and Shevchenko, A. 2001. Evaluation of the efficiency of in-gel digestion of proteins by peptide isotopic labeling and MALDI mass spectrometry. Anal. Biochem. 296: 279-283.
- (2001) Anal. Biochem , vol.296 , pp. 279-283
- Shevchenko, A.¹ Shevchenko, A.²

6
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- In-gel digestion for mass spectrometric characterization of proteins and proteomes
- Shevchenko A, Tomas, H., Havlis, J., Olsen, J.V., and Mann, M. 2006. In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nat. Protoc. 1: 2856-2860.
- (2006) Nat. Protoc , vol.1 , pp. 2856-2860
- Shevchenko, A.¹ Tomas, H.² Havlis, J.³ Olsen, J.V.⁴ Mann, M.⁵

7
- 0029927505
- Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels
- Shevchenko, A., Wilm, M., Vorm, O., and Mann, M. 1996. Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels. Anal. Chem. 68: 850-858.
- (1996) Anal. Chem , vol.68 , pp. 850-858
- Shevchenko, A.¹ Wilm, M.² Vorm, O.³ Mann, M.⁴

8
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- SDS-PAGE of proteins
- doi: 10.1101/pdb.prot4313
- Simpson, R.J. 2006. SDS-PAGE of proteins. Cold Spring Harb. Protoc. doi: 10.1101/pdb.prot4313.
- (2006) Cold Spring Harb. Protoc
- Simpson, R.J.¹

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